Prevalência e fatores de risco associados à infecção por Toxoplasma gondii em granjas suinícolas tecnificadas no Estado de Alagoas

Valença, Rômulo Menna Barreto; Mota, Rinaldo Aparecido; Anderlini, Giulliano Aires; Faria, E. B.; Cavalcanti, Érica F.S.T.F.; Albuquerque, Pedro Paulo Feitosa de; Neto, Orestes Luiz de Souza; Guerra, Maria Madalena Pessoa

doi:10.1590/s0100-736x2011000200005

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…However, the occurrence of toxoplasmosis in pigs found in this study was higher than those observed in previous studies: 36.2% in Paraíba (Azevedo et al 2010); 26.9% in Alagoas (Valença et al 2011); 18.2% in Bahia (Bezerra et al 2009); 12.8% in Mato Grosso (Muraro et al 2010); 12.6% in Paraná (Santos et al 2015) and 12.5% in Pernambuco (Fernandes et al 2012). But was lower than observed in Rio de Janeiro (65.8%; Bonna et al 2006) and Rio Grande do Sul (86.0%; Silva et al 2003).…”

Section: Discussioncontrasting

confidence: 85%

“…The presence of rodents on pig farms was described by Valença et al (2011) as a risk factor not only because rats attract cats, which could eliminate oocysts in their feces and contaminate the environment, but also because these animals could be infected by ingesting rodents with tissue cysts. Although the presence of rodents did not represent risk for pig infection in the present study (OR=0.6, CI 95%, 0.40-0.90), the epidemiological questionnaire revealed that 41.7% of the animals from the farm with rodent presence were positive for T. gondii, which suggests a participation of the rodents in pig infection.…”

Section: Discussionmentioning

confidence: 99%

“…Viable forms of the parasite have been isolated from a great variety of meat products and serological studies in Brazil showed a wide distribution of this infection in pigs (Bonna et al 2006, Millar et al 2008, Muraro et al 2010, Azevedo et al 2010, Valença et al 2011, Sousa et al 2014.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of Toxoplasma gondii and risk factors for infection in pigs raised and slaughtered in the Triângulo Mineiro region, Minas Gerais, Brazil

Marques-Santos

Amendoeira²,

Carrijo

et al. 2017

Pesq. Vet. Bras.

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The Triângulo Mineiro region from Minas Gerais state, is an important meat-exporting region of Brazil and data about Toxoplasma gondii infection in pigs raised and slaughtered in this area are scarce. Therefore, the aim of this study was to evaluate the occurrence of T. gondii in swine and establish the risk factors associated with the infection. Samples were collected from 600 pigs raised under intensive system in farms located at three different counties (Carmo do Paranaíba, Patrocínio and Perdizes). The samples were submitted to indirect hemagglutination antibody test with dilution of 1:32 and to indirect immunofluorescence antibody test with a cutoff of 1:64. The occurrence of positive pig was 3.3% (n=20) and 51.8% (n=311) respectively. A significant difference was observed between toxoplasmatic infection and factors such as lineage, animal origin, size of the farm, collective raising with others species, presence of rodents and type of water offered (p≤0.05). There was no difference between gender and the farm goals. The results demonstrated an occurrence of anti-T. gondii antibodies higher than expected for intensive pig raising system on the studied area, which could indicate a possible sanitary management problem on the studied proprieties. Improvements on the raising techniques are necessary to reduce T. gondii infection sources.

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Section: Discussioncontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Occurrence of Toxoplasma gondii and risk factors for infection in pigs raised and slaughtered in the Triângulo Mineiro region, Minas Gerais, Brazil

Marques-Santos

Amendoeira²,

Carrijo

et al. 2017

Pesq. Vet. Bras.

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“…No entanto, a análise estatística realizada na amostra em estudo não evidenciou associação de riscos significativos quanto o acesso de gatos em depósitos, uma vez que o intervalo de confiança não foi significativo. Este dado está de acordo com outros trabalhos, como Valença et al (2011), em granjas de suínos e Pereira et al (2012), em propriedades de ovinos e caprinos, os quais também demonstraram que não houve esta associação à infecção pelo T. gondii acesso de gatos em locais de armazenamento de alimentos. Conforme Cademartori et al (2008), a maioria dos estudos epidemiológicos já realizados não são uniformes quanto à associação entre a infecção por T. gondii e o convívio com gatos, relatando que também não foi constatada essa associação em trabalho realizado com gestantes em Pelotas/RS.…”

Section: Discussionunclassified

Prevalência de anticorpos e fatores de risco associados à infecção por Toxoplasma gondii em galinhas domésticas da zona rural de Santa Maria, Rio Grande do Sul

et al. 2018

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RESUMO: Toxoplasma gondii é um protozoário apicomplexa que infecta animais de sangue quente, podendo ser considerado um dos principais parasitas capazes de infectar os seres humanos. Galinhas domésticas podem ser facilmente infectadas por protozoários, uma vez que estas podem ingerir oocistos encontrados no solo, sendo consideradas boas indicadoras de contaminação ambiental por T. gondii. O objetivo deste estudo foi determinar a presença de anticorpos anti-T. gondii em galinhas domésticas criadas extensivamente e avaliar os fatores de risco associados ao protozoário, na zona rural de Santa Maria, RS, Brasil. No período de março de 2013 a fevereiro 2014 foram coletadas 597 amostras de sangue de galinhas domésticas em 74 propriedades, oriundas de nove estratos que representam cada distrito da zona rural. Para avaliar os fatores de risco, nessas propriedades foi aplicado um questionário epidemiológico aos moradores. As amostras de soro foram testadas por imunofluorescência indireta, e 49,2% (294/597) foram positivas para anticorpos anti-T. gondii, com títulos variando de 16 a 4096. Das 74 propriedades analisadas, em 63 (85,1%) houve relatos que os gatos têm acesso ao deposito de alimentos, com associação significativa quando associado à presença de galinhas positivas (p=0,04) e o OR de 4,07. A variável “abate de animais” (aves e bovinos), em 51 (68,9%) das propriedades foi relatado o abate de bovinos e aves na propriedade, com valor de p significativo (p=0,05). A maioria das propriedades 59 (79,7%) foi relatada a presença de gatos domésticos, o que poderia estar associada com a alta soroprevalência encontrada em galinhas e a taxa de contaminação ambiental. A elevada prevalência de anticorpos encontrada neste estudo, além da alta frequência de propriedades com casos positivos, sugere uma grande contaminação ambiental nos distritos pesquisados, sendo assim um risco potencial para a saúde humana e animal.

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“…In immunocompromised patients, such as those with HIV/AIDS, infection can cause life-threatening encephalitis and be fatal if not recognized and treated soon after infection (Montoya and Liesenfeld, 2004). Infection with T. gondii in animals has resulted in economic losses to livestock or animal husbandry (Caldeira et al, 2011; Valença et al, 2011). Infection occurs through ingestion of food or water contaminated with oocysts and eating undercooked or raw meat containing tissue cysts (Hill and Dubey, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro

Liu

Sun

et al. 2017

Front. Microbiol.

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Toxoplasmosis, which is one of the most widespread zoonoses worldwide, has a high incidence and infection can result in severe disease in humans and livestock. Citrate synthase (CS) is a component of nearly all living cells that plays a vital role in the citric acid cycle, which is the central metabolic pathway of aerobic organisms. In the present study, the citrate synthase I gene of Toxoplasma gondii (T. gondii) (TgCSI) was cloned and characterized. The TgCSI gene had an open reading frame of 1665 bp nucleotides encoding a 555 amino acid protein with a molecular weight of 60 kDa. Using western blotting assay, the recombinant protein was successfully recognized by the sera of rats experimentally infected with T. gondii, while the native protein in the T. gondii tachyzoites was detected in sera from rats immunized with the recombinant protein of TgCSI. Binding of the protein to murine macrophages was confirmed by immuno fluorescence assay. Following incubation of macrophages with rTgCSI, the rTgCSI protein was found to have a dual function, with low concentrations (5–10 μg/mL) enhancing phagocytosis and high levels (80 μg/mL) inhibiting phagocytosis. Investigation of murine macrophage apoptosis illustrated that 5 μg/mL rTgCSI protein can significantly induce early apoptosis and late stage apoptosis (*p < 0.05), while 10 μg/mL rTgCSI protein significantly induced early apoptosis, but had no effect on late stage of apoptosis (**p < 0.01), and 80 μg/mL rTgCSI protein inhibited late stage apoptosis of macrophages (*p < 0.05). Cytokine detection revealed that the secretion of interleukin-10, interleukin-1β, transforming growth factor-β1 and tumor necrosis factor-α of macrophages increased after the cells were incubated with all concentration of rTgCSI, with the exception that 5 μg/mL rTgCSI had no effect on the secretion of interleukin-10 and interleukin-1β. However, secretion of NO and cell proliferation of the macrophages were substantially reduced. Taken together, these results suggested that TgCSI can affect the immune functions of murine macrophages by binding to the cells in vitro.

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Prevalência e fatores de risco associados à infecção por Toxoplasma gondii em granjas suinícolas tecnificadas no Estado de Alagoas

Cited by 6 publications

References 19 publications

Occurrence of Toxoplasma gondii and risk factors for infection in pigs raised and slaughtered in the Triângulo Mineiro region, Minas Gerais, Brazil

Occurrence of Toxoplasma gondii and risk factors for infection in pigs raised and slaughtered in the Triângulo Mineiro region, Minas Gerais, Brazil

Prevalência de anticorpos e fatores de risco associados à infecção por Toxoplasma gondii em galinhas domésticas da zona rural de Santa Maria, Rio Grande do Sul

Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro

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