Prevalencia, microbiología y patrones de sensibilidad a los antifúngicos de los aislamientos orales de Candida que colonizaban o infectaban a pacientes mexicanos con infección por VIH o sida y a personas sanas

Sánchez-Vargas, Luis Octavio; Ortiz-López, Natalia Guadalupe; Villar, María; Moragues, María Dolores; Aguirre, J.M.; Cashat-Cruz, Miguel; López-Ribot, José L.; Gaitán-Cepeda, Luis Alberto; Quindós, G.

doi:10.1016/s1130-1406(05)70014-0

Cited by 50 publications

(17 citation statements)

References 44 publications

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“…Less pathogenic strains of candidal species were found to be associated with oral candidiasis [1-3]. Although several species Candida comprise part of the oral commensal microflora, their emergence may at time cause opportunistic infections among debilitated and immunocompromised hosts [4,5]. The interactions between candida and the host are extremely complex.…”

Section: Introductionmentioning

confidence: 99%

Antifungal susceptibility and growth inhibitory response of oral Candida species to Brucea javanica Linn. extract

Nordin

Harun

Razak

2013

BMC Complement Altern Med

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BackgroundCandida species have been associated with the emergence of resistant strains towards selected antifungal agents. Plant products have been used traditionally as alternative medicine to ease candidal infections. The present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibiting effect of Brucea javanica seeds extract against Candida species.MethodsA total of seven Candida strains that includes Candida albicans ATCC14053, Candida dubliniensis ATCCMYA-2975, Candida glabrata ATCC90030, Candida krusei ATCC14243, Candida lusitaniae ATCC64125, Candida parapsilosis ATCC22019 and Candida tropicalis ATCC13803 were used in this study. The antifungal activity, minimum inhibitory concentration and minimum fungicidal concentration of B. javanica extract were evaluated. Each strain was cultured in Yeast Peptone Dextrose broth under four different growth environments; (i) in the absence and presence of B. javanica extract at respective concentrations of (ii) 1 mg/ml (iii) 3 mg/ml and (iv) 6 mg/ml. The growth inhibitory responses of the candidal cells were determined based on changes in the specific-growth rates (μ) and doubling time (g). The values in the presence of extract were computed as percentage in the optical density relative to that of the total cells suspension in the absence of extract.ResultsB. javanica seeds extract exhibited antifungal properties. C. tropicalis showed the highest growth rate; 0.319 ± 0.002 h-1, while others were in the range of 0.141 ± 0.001 to 0.265 ± 0.005 h-1. In the presence of extract, the lag and log phases were extended and deviated the μ- and g-values. B. javanica extract had significantly reduced the μ-values of C. dubliniensis, C. krusei and C. parapsilosis at more than 80% (ρ < 0.05), while others were reduced within the range of 2.28% to 57.05%. The g-values of most candidal strains were extended and significantly reduced (ρ < 0.05) in relative to the untreated. The candidal population was reduced from an average of 10 x 106 to 6 x 106 CFU/ml.ConclusionsB. javanica extract exhibited in vitro antifungal activity against seven oral Candida species. The fungistatic and growth inhibiting effects of B. javanica extract have shown that it has potential to be considered as a promising candidate for the development of antifungal agent in oral health products.

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Section: Introductionmentioning

confidence: 99%

Antifungal susceptibility and growth inhibitory response of oral Candida species to Brucea javanica Linn. extract

Nordin

Harun

Razak

2013

BMC Complement Altern Med

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“…The pseudomembranous, angular cheilitis, and erythematous forms of candidiasis were observed in the patients in our work. These clinical forms of the disease have been reported in studies of oral infections in patients with AIDS in different regions of the world 5 , 11 , 14 , 15 , 17 , 18 and are considered the most common forms of these lesions. The pseudomembranous type was the most common form of oral candidiasis in the patients, occurring in multiple anatomic sites in 6 individuals.…”

Section: Discussionmentioning

confidence: 81%

“…The resultant decrease in systemic and mucosal immune integrity is, in turn, associated with an increased frequency of oral lesions 3 . The degree of immunodeficiency determines the recurrence and severity of this mycotic infection 2 , 3 , 7 , 15 .…”

Section: Introductionmentioning

confidence: 99%

“…The correct identification of the etiological agent can indicate the best therapeutic option for the treatment of these patients. This identification is important because some opportunistic yeast species respond differently to antifungal agents used for treatment 1 , 15 . C. glabrata and C. krusei are frequently resistant to fluconazole, and a significant proportion of isolates from these species may also have a reduced susceptibility to amphotericin B.…”

Section: Introductionmentioning

confidence: 99%

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Incidence and anatomic localization of oral candidiasis in patients with AIDS hospitalized in a public hospital in Belo Horizonte, MG, Brazil

et al. 2008

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The objective of this study was to evaluate the incidence, anatomic localization and yeast species isolated from each clinical type of oral candidiasis. The clinical samples were obtained from 67 patients with AIDS with CD4 cell counts below 200 cells/mm3 and hospitalized in a public hospital (Eduardo de Menezes Hospital) in the city of Belo Horizonte, MG, Brazil. Yeasts were isolated using Chromagar® Candida. The results show that 50.7% of these patients had oral candidiasis. The pseudomembranous form was the most frequent clinical manifestation of oral candidiasis, followed by the erythematous and angular cheilite forms. The most common site of these clinical forms of oral candidiasis was the tongue. Candida albicans was the most common yeast species isolated from the lesions. However, other species were also found to be associated with these forms of oral candidiasis.

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“…Candida albicans has often been reported as the predominant species associated with superficial and systemic fungal infections. 1 Of late, however, the prevalence of C. albicans has surpassed by the emergence of non- Candida albicans Candida species, 2,3,4 and increased prescription of antifungal agents 5 has been suggested to be a contributing factor. The increased number of compromised patients with common endocrine disorders such as diabetes mellitus, 6 with malnutrition and with smoking habits, 7 has been identified to be primarily responsible for the development of candidal infections.…”

Section: Introductionmentioning

confidence: 99%

Growth inhibitory response and ultrastructural modification of oral-associated candidal reference strains (ATCC) by Piper betle L. extract

et al. 2014

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Candida species have been associated with the emergence of strains resistant to selected antifungal agents. Plant products have been used traditionally as alternative medicine to ease mucosal fungal infections. This study aimed to investigate the effects of Piper betle extract on the growth profile and the ultrastructure of commonly isolated oral candidal cells. The major component of P. betle was identified using liquid chromatography-mass spectrophotometry (LC-MS/MS). Seven ATCC control strains of Candida species were cultured in yeast peptone dextrose broth under four different growth environments: (i) in the absence of P. betle extract; and in the presence of P. betle extract at respective concentrations of (ii) 1 mg⋅mL−1; (iii) 3 mg⋅mL−1; and (iv) 6 mg⋅mL−1. The growth inhibitory responses of the candidal cells were determined based on changes in the specific growth rates (µ). Scanning electron microscopy (SEM) was used to observe any ultrastructural alterations in the candida colonies. LC-MS/MS was performed to validate the presence of bioactive compounds in the extract. Following treatment, it was observed that the µ-values of the treated cells were significantly different than those of the untreated cells (P<0.05), indicating the fungistatic properties of the P. betle extract. The candidal population was also reduced from an average of 13.44×106 to 1.78×106 viable cell counts (CFU)⋅mL−1. SEM examination exhibited physical damage and considerable morphological alterations of the treated cells. The compound profile from LC-MS/MS indicated the presence of hydroxybenzoic acid, chavibetol and hydroxychavicol in P. betle extract. The effects of P. betle on candida cells could potentiate its antifungal activity.

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Prevalencia, microbiología y patrones de sensibilidad a los antifúngicos de los aislamientos orales de Candida que colonizaban o infectaban a pacientes mexicanos con infección por VIH o sida y a personas sanas

Cited by 50 publications

References 44 publications

Antifungal susceptibility and growth inhibitory response of oral Candida species to Brucea javanica Linn. extract

Antifungal susceptibility and growth inhibitory response of oral Candida species to Brucea javanica Linn. extract

Incidence and anatomic localization of oral candidiasis in patients with AIDS hospitalized in a public hospital in Belo Horizonte, MG, Brazil

Growth inhibitory response and ultrastructural modification of oral-associated candidal reference strains (ATCC) by Piper betle L. extract

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