Prevelance of Brucellosis among Febrile Negative Malaria Patients by PCR in Northern Kordofan State, Sudan

Abdelhady, Reham; Anan, Khalid; Elhussein, A. M.; Hussien, Mohammed; Mohammed, ELamain E; Elkhidir, Isam M; Abdelhaleem, Aymen

doi:10.4172/2327-5073.1000293

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…DNA extraction: DNA extracted was done by saturated sodium chloride method according to Reham et al 7 modified from Miller et al 8 By re-suspend the blood sample in red cell lysis buffer, the supernatant was discarded after centrifugation of the mixture then the white cell lysis buffer was added and incubated for 1hour at 65°C with 10%SDS and 20µl of protein's K. 8 6M NaCl added before cold chloroform was added and centrifuged for 6minute.…”

Section: Molecular Methodsmentioning

confidence: 99%

Validity of LAMP and molecular detection of human brucellosis among febrile negative malaria patients in Northern Kordofan State, Sudann

Enanm¹

2018

JBMOA

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Background: Brucellosis is a serious problem for man in contact with livestock and the diagnosis of this disease still depends on Cultivation and Isolation methods which can consume long time for diagnosis. Nowadays the invention of Loop-mediated isothermal amplification (LAMP) and other molecular technique can play role in rapid diagnosis and saving time with highly sensitive and specific results. Aim: To determine the validity of new LAMP primer design by Mast group comparing to nested PCR technique. The old valid LAMP primer designed according to the published sequence of omp25 (GenBank accession No. gi 769744) to help in diagnosis of brucellosis among malaria negative febrile patients in Northern kordofan. Methods: Seventy five blood samples were collected from febrile malaria negative patients of different ages from North Kordofan State, and examined by nested PCR and LAMP methods. Results: The results showed that old LAMP primer came positive for 40 and negative for 35 of the patient's samples in other hand the new LAMP primer came positive for 65 patient's samples and negative for only 10 samples. Conclusion: The sensitivity and specificity of new LAMP primer design by (Mast, Reinfeld, Germany) for detection of Brucella spp. was tested and validity of these primer show much better results when compared with nested PCR and another LAMP primer used. KAPPA value calculated to show the degree of agreement compare to the new primer results

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Section: Molecular Methodsmentioning

confidence: 99%

Validity of LAMP and molecular detection of human brucellosis among febrile negative malaria patients in Northern Kordofan State, Sudann

Enanm¹

2018

JBMOA

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“…A recent proof-of-principle of ConnDx was provided in Samburu, Kenya, in which RDTs interpreted by the Fio Deki Reader™ were used to diagnose brucellosis in remote populations [ 27 ]. Brucellosis, a rare bacterial zoonotic illness, is often underdiagnosed due to having similar febrile symptoms as malaria [ 28 ]. Patients testing positive were directly linked to funds through their respective mobile health wallets to allow for payment of drugs for the pertinent diseases, conditional to a positive diagnosis of these diseases.…”

Section: Introductionmentioning

confidence: 99%

Connected diagnostics to improve accurate diagnosis, treatment, and conditional payment of malaria services in Kenya

Duijn

Siteyi²,

Smith

et al. 2021

BMC Med Inform Decis Mak

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Background In sub-Saharan Africa, the material and human capacity to diagnose patients reporting with fever to healthcare providers is largely insufficient. Febrile patients are typically treated presumptively with antimalarials and/or antibiotics. Such over-prescription can lead to drug resistance and involves unnecessary costs to the health system. International funding for malaria is currently not sufficient to control malaria. Transition to domestic funding is challenged by UHC efforts and recent COVID-19 outbreak. Herewith we present a digital approach to improve efficiencies in diagnosis and treatment of malaria in endemic Kisumu, Kenya: Connected Diagnostics. The objective of this study is to evaluate the feasibility, user experience and clinical performance of this approach in Kisumu. Methods Our intervention was performed Oct 2017–Dec 2018 across five private providers in Kisumu. Patients were enrolled on M-TIBA platform, diagnostic test results digitized, and only positive patients were digitally entitled to malaria treatment. Data on socio-demographics, healthcare transactions and medical outcomes were analysed using standard descriptive quantitative statistics. Provider perspectives were gathered by 19 semi-structured interviews. Results In total 11,689 febrile patients were digitally tested through five private providers. Malaria positivity ranged from 7.4 to 30.2% between providers, significantly more amongst the poor (p < 0.05). Prescription of antimalarials was substantially aberrant from National Guidelines, with 28% over-prescription (4.6–63.3% per provider) and prescription of branded versus generic antimalarials differing amongst facilities and correlating with the socioeconomic status of clients. Challenges were encountered transitioning from microscopy to RDT. Conclusion We provide full proof-of-concept of innovative Connected Diagnostics to use digitized malaria diagnostics to earmark digital entitlements for correct malaria treatment of patients. This approach has large cost-saving and quality improvement potential.

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“…A recent proof-of-principle of ConnDx, was provided in Samburu, Kenya, in which RDTs interpreted by the Fio Deki Reader TM were used to diagnose brucellosis in remote populations [24]. Brucellosis, a rare bacterial zoonotic illness, is often underdiagnosed due to having similar febrile symptoms as malaria [25]. Patients testing positive were directly linked to funds through their respective mobile health wallets to allow for payment of drugs for the pertinent diseases, conditional to positive diagnosis of these diseases.…”

Section: Introductionmentioning

confidence: 99%

Connected Diagnostics to Improve Accurate Diagnosis, Treatment, and Conditional Payment of Malaria Services in Kenya.

Duijn

Siteyi²,

Smith

et al. 2020

Preprint

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Abstract Background: In sub-Saharan Africa, the material and human capacity to diagnose patients reporting with fever to healthcare providers is largely insufficient. Febrile patients are typically treated presumptively with antimalarials and/or antibiotics. Such over-prescription can lead to drug resistance and involves unnecessary costs to the health system. International funding for malaria is decreasing and transition to domestic funding is challenged by UHC efforts and recent COVID-19 outbreak. Herewith we present a digital approach to markedly improve efficiencies in diagnosis and treatment of malaria in endemic Kisumu, Kenya. The objective of this study is to evaluate feasibility, user experience, clinical performance and of Connected Diagnostics in Kisumu and to assess over-prescription of antimalarials. Methods: Our intervention was performed Oct 2017 – Dec 2018 across seven providers in Kisumu. Patients were enrolled on M-TIBA platform, diagnostic test results digitized, and only positive patients were digitally entitled for malaria treatment. Data on socio-demographics, healthcare transactions and medical outcomes were analysed using standard descriptive quantitative statistics. Provider perspectives were gathered by 19 semi-structured interviews. Results: In total 11,689 febrile patients were tested. Malaria positivity rates ranged from 7.4% to 30.2% between providers, with significantly more positive cases amongst the poor (p< 0.05). Over-prescription of antimalarials was 28%, fluctuating between 4.6% to 63.3% per provider. Prescription of branded versus generic antimalarials was dichotomous. Challenges were encountered transitioning from microscopy to RDT. Conclusion: We provide full proof-of-concept of innovative Connected Diagnostics to use digitized malaria diagnostics to earmark digital entitlements for correct malaria treatment of patients. This approach has large cost-saving and quality improving potential.

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Prevelance of Brucellosis among Febrile Negative Malaria Patients by PCR in Northern Kordofan State, Sudan

Cited by 3 publications

References 17 publications

Validity of LAMP and molecular detection of human brucellosis among febrile negative malaria patients in Northern Kordofan State, Sudann

Validity of LAMP and molecular detection of human brucellosis among febrile negative malaria patients in Northern Kordofan State, Sudann

Connected diagnostics to improve accurate diagnosis, treatment, and conditional payment of malaria services in Kenya

Connected Diagnostics to Improve Accurate Diagnosis, Treatment, and Conditional Payment of Malaria Services in Kenya.

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