Prevent Effects of Lactobacillus Fermentum HY01 on Dextran Sulfate Sodium-Induced Colitis in Mice

Abstract

The aim of this study is to assess the preventive effects of Lactobacillus fermentum HY01 (LF-HY01) to dextran sulfate sodium induced-colitis. We observed the ratio of colon weight to its length, colon pathological changes, and the concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6) in serum. We also took account of the protein levels of IκBα, NF-κB p65, iNOS, and COX-2, and we measured the best effects of different doses of Lactobacillus fermentum HY01 (low dose group was 109 CFU/kg·bw, high dose group was 1010 CFU/kg·bw) on dextran sulfate sodium-induced colitis mice. The results were remarkable, suggesting that Lactobacillus fermentum HY01 had significant preventive effects in dextran sulfate sodium induced-colitis; simultaneously, the high dose group showed the best results among other groups. It can effectively alleviate the shortened colon length, reduce the ratio of colon weight to its length, reduce edema, inflammatory cells infiltration, and colon mucosa injury, and play an important role in the down-regulation of concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6). Above all, Lactobacillus fermentum HY01 shows promising prevention for IκBα degradation, inhibition of NF-κB p65 phosphorylation cascades, and decreases the protein levels of iNOS and COX-2 as well.

New Progress Regarding the Use of Lactic Acid Bacteria as Live Delivery Vectors, Treatment of Diseases and Induction of Immune Responses in Different Host Species Focusing on Lactobacillus Species

“…The administration of microbial metabolites for the prevention and treatment of aberrant immune response is also gaining importance [106,150]. The market size of probiotics has to the highest degree increased consumer anxiety concerning health-promoting effect of nutraceuticals [38,138,151].…”

Process Design of the Antioxidant Shuidouchi and Its Effect on Preventing Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice via Antioxidant Activity

“…The standard curve of the soybean isoflavones content was ultimately obtained (genistein meter, Figure 2). the primary antibodies (IL-6, IL-12, and TNF-α, Thermo Fisher Scientific) were incubated at 25 °C for 2 h, and the secondary antibody was incubated at 25 °C for 1 h. Finally, Supersignal West Pico PLUS was used to spray the PVDF film, followed by placin into iBright FL1000 (Thermo Fisher Scientific) for observation [17].…”

“…After feeding distilled water for one week, all other mice except for the normal group received 4% (w/v) DSS solution as drinking water during the fifth week. Except for the third and fifth weeks, all mice received distilled water as drinking water for the rest of the time [16,17]. During the entire experimental duration of five weeks, the SDC-L and SDC-H groups received 2 and 4 g/kg of extract of Shuidouchi daily after the optimization of crafts.…”

“…A total of 40 cycles were conducted after which the reaction conditions were as follows: 95 • C, 60 s; 95 • C, 15 s; 55 • C, 30 s; and, 7 • C, 35 s (StepOnePlus Real-Time PCR System, Thermo Fisher Scientific, Waltham, MA, USA). Relative gene expression was calculated using the 2 -∆∆Ct method, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal reference [17]. Table 5.…”

“…The prestained protein ladder and samples were put into the sample holes of the rubber sheet, and then the loaded SDS-PAGE gel was subjected to 50 min vertical gel electrophoresis. The PVDF (polyvinylidene fluoride) membrane was activated by methanol for 1 min and then the transmembrane was closed by TBST (tris buffered saline tween) solution containing 5% skim milk for 1 h. After closure, the PVDF membrane was washed by TBST, the primary antibodies (IL-6, IL-12, and TNF-α, Thermo Fisher Scientific) were incubated at 25 • C for 2 h, and the secondary antibody was incubated at 25 • C for 1 h. Finally, Supersignal West Pico PLUS was used to spray the PVDF film, followed by placin into iBright FL1000 (Thermo Fisher Scientific) for observation [17].…”