Preventative Effects of Sodium Alginate on Indomethacin-induced Small-intestinal Injury in Mice

Horibe, Sayo; Tanahashi, Takao; Kawauchi, Shoji; Mizuno, Shigeto; Rikitake, Yoshiyuki

doi:10.7150/ijms.16232

Cited by 31 publications

(31 citation statements)

References 28 publications

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“…qPCR was performed as described previously (Horibe et al . ). Total RNA was extracted using ReliaPrep ® RNA Miniprep Systems (Promega KK, Fitchburg, WI, USA, Cat# 04080‐24).…”

Section: Methodsmentioning

confidence: 97%

Involvement of aquaporin‐4 in laminin‐enhanced process formation of mouse astrocytes in 2D culture: Roles of dystroglycan and α‐syntrophin in aquaporin‐4 expression

Sato

Horibe

Kawauchi

et al. 2018

Journal of Neurochemistry

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In the central nervous system, astrocytes extend endfoot processes to ensheath synapses and microvessels. However, the mechanisms underlying this astrocytic process extension remain unclear. A limitation of the use of 2D cultured astrocytes for such studies is that they display a flat, epithelioid morphology, with no or very few processes, which is markedly different from the stellate morphology observed in vivo. In this study, we obtained 2D cultured astrocytes with a rich complexity of processes using differentiation of neurospheres in vitro. Using these process-bearing astrocytes, we showed that laminin, an extracellular matrix molecule abundant in perivascular sites, efficiently induced process formation and branching. Specifically, the numbers of the first- and second-order branch processes and the maximal process length of astrocytes were increased when cultured on laminin, compared with when they were cultured on poly-L-ornithine or type IV collagen. Knockdown of dystroglycan or α-syntrophin, constituent proteins of the dystrophin-glycoprotein complex that provides a link between laminin and the cytoskeleton, using small interference RNAs inhibited astrocyte process formation and branching, and down-regulated expression of the water channel aquaporin-4 (AQP4). Direct knockdown and a specific inhibitor of AQP4 also inhibited, whereas over-expression of AQP4 enhanced astrocyte process formation and branching. Knockdown of AQP4 decreased phosphorylation of focal adhesion kinase (FAK) that is critically implicated in actin remodeling. Collectively, these results indicate that the laminin-dystroglycan-α-syntrophin-AQP4 axis is important for process formation and branching of 2D cultured astrocytes. OPEN PRACTICES: This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/. Read the Editorial Highlight for this article on page 436.

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“…qPCR was performed as described previously (Horibe et al . ). Total RNA was extracted using ReliaPrep ® RNA Miniprep Systems (Promega KK, Fitchburg, WI, USA, Cat# 04080‐24).…”

Section: Methodsmentioning

confidence: 97%

Involvement of aquaporin‐4 in laminin‐enhanced process formation of mouse astrocytes in 2D culture: Roles of dystroglycan and α‐syntrophin in aquaporin‐4 expression

Sato

Horibe

Kawauchi

et al. 2018

Journal of Neurochemistry

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“…Sodium alginate from brown seaweed and pectin from plants are soluble dietary fiber and have been used as a hemostatic agent to treat gastrointestinal bleeding due to gastric ulcers (Horibe et al, 2016;. To solve the intestinal flatulence that may be caused by the intake of high-dose extract, RLE with pectin and sodium alginate with boiling water was prepared into a gelled food for the mice.…”

Section: Mice Endurance Capacity and Weight Changes In Mice Fed Extramentioning

confidence: 99%

“…Nonsteroidal anti-inflammatory drugs (indomethacin, diclofenac, and loxoprofen) often have a limited use due to serious adverse effects on the gastrointestinal tract and kidneys. However, small-intestinal injury induced by these drugs was restored and inhibited by feeding powdered CE-2 containing 5% (w/v) sodium alginate (Horibe et al, 2016) and a regular diet containing 1-10% (w/v) pectin (Satoh & Urushidani, 2016). This protection mechanism is also involved in decreasing the mRNA expression of the mucin gene family (Muc1, Muc2, Muc3, and Muc4) in the small intestine (Horibe et al, 2016).…”

Section: Mice Endurance Capacity and Weight Changes In Mice Fed Extramentioning

confidence: 99%

“…However, small-intestinal injury induced by these drugs was restored and inhibited by feeding powdered CE-2 containing 5% (w/v) sodium alginate (Horibe et al, 2016) and a regular diet containing 1-10% (w/v) pectin (Satoh & Urushidani, 2016). This protection mechanism is also involved in decreasing the mRNA expression of the mucin gene family (Muc1, Muc2, Muc3, and Muc4) in the small intestine (Horibe et al, 2016). Moreover, calcium-gelled, alginate-pectin beverage may provide satiety to reduce energy intake in nondieting over weight and obese women (Pelkman et al, 2007).…”

Section: Mice Endurance Capacity and Weight Changes In Mice Fed Extramentioning

confidence: 99%

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Determination of active compounds in raspberry leaf extracts and the effects of extract intake on mice

et al. 2020

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Twelve typical polyphenols in raspberry leaf powder (RLP), raspberry leaf extract 1 (RLE) and raspberry leaf extract 2 (RLE-H) were analyzed by HPLC-MS. The sum of their contents in three samples were in the following order: RLP (8.6 mg g -1 ) < RLE (35.0 mg g -1 ) < RLE-H (44.1 mg g -1 ). Ellagic acid and quercetin-3-D-glucuronide were the principal compounds in the samples and accounted for more than 75% of the sum content. A gelled mixture of RLE, pectin, and sodium alginate administrated to mice via gavage resulted in reduced weight gain and adiposity percentage, increased teste percentage, prolonged exhaustion time, furthermore alleviated intestinal flatulence possibly induced by RLE or RLE-H alone. These results could help in making rational use of this high polyphenol extract from raspberry leaves.

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“…Primer sequences are shown in Table 2. Relative expression was calculated using the delta-delta Ct method with β-actin used as a reference gene [13].…”

Section: Real-time Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Anti-inflammatory Effect of JBP485 on Dextran Sulfate Sodium-induced Colitis in Mice

Horibe¹,

Kawauchi²,

Yasuike³

et al. 2017

J. Biomed

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JBP485 (cyclo-trans-4-L-hydroxyprolyl-L-serine), a dipeptide first isolated from Laennec that is a proprietary product made from the hydrolysate of human placenta, has been reported to have several biological properties, such as anti-hepatotoxic, anti-inflammatory and anti-apoptotic activities. However, the effect of JBP485 on inflammatory bowel disease has not yet been reported. Here we investigated the effects of simultaneous and subsequent administration of JBP485 on dextran sulfate sodium (DSS)-induced colitis in mice. In both experimental protocols, DSS-induced colitis was established by giving mice drinking water containing 2% (w/v) DSS for 5 days. JBP485 (25 mg/kg) was orally administered once a day during and after DSS treatment for 5 days in the simultaneous administration experiment while after DSS treatment for 5 days in the subsequent administration experiment. In the simultaneous administration experiment, JBP485 did not improve disease progression, such as weight loss, diarrhea and visible fecal blood, as well as shortening of the colon, but reduced the DSS-induced injury and up-regulation of tumor necrosis factor-α, interleukin-1β, cyclooxygenase-2, monocyte chemotactic protein 1 and C-X-C motif chemokine ligand 1 mRNAs in the colon. In the subsequent administration experiment, however, JBP485 did not show any effects on DSS-induced colitis. These results demonstrated that simultaneous administration but not subsequent administration of JBP485 had anti-inflammatory effects on DSS-induced colitis in mice.

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Preventative Effects of Sodium Alginate on Indomethacin-induced Small-intestinal Injury in Mice

Cited by 31 publications

References 28 publications

Involvement of aquaporin‐4 in laminin‐enhanced process formation of mouse astrocytes in 2D culture: Roles of dystroglycan and α‐syntrophin in aquaporin‐4 expression

Involvement of aquaporin‐4 in laminin‐enhanced process formation of mouse astrocytes in 2D culture: Roles of dystroglycan and α‐syntrophin in aquaporin‐4 expression

Determination of active compounds in raspberry leaf extracts and the effects of extract intake on mice

Anti-inflammatory Effect of JBP485 on Dextran Sulfate Sodium-induced Colitis in Mice

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