Preventing further misuse of the ELISA technique and misinterpretation of serological antibody assay data

Terato, Kuniaki; Do, Christopher; Chang, Jessica; Waritani, Takaki

doi:10.1016/j.vaccine.2016.08.007

Cited by 33 publications

(23 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, data influenced largely by this BG noise reaction [1] has led to numerous uncertain conclusions and misunderstandings as discussed [2] , [3] , [4] . To prevent further misuse of the ELISA technique and misinterpretation of serological antibody assay data, it is important to reconsider the principle of the immunoassay system and all types of non-specific reactions involved [5] . The following are basic issues that all ELISA users must take into consideration before setting up an ELISA system for assaying antibodies.…”

Section: Description Of Protocolmentioning

confidence: 99%

An ELISA protocol to improve the accuracy and reliability of serological antibody assays

Waritani¹,

Chang²,

McKinney³

et al. 2017

MethodsX

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Description Of Protocolmentioning

confidence: 99%

An ELISA protocol to improve the accuracy and reliability of serological antibody assays

Waritani¹,

Chang²,

McKinney³

et al. 2017

MethodsX

Self Cite

View full text Add to dashboard Cite

show abstract

“…Unfortunately, these studies were conducted using immunoassay systems such as radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) without considering the intense background (BG) noise reaction caused by hydrophobic binding of immunoglobulins and immune-complexes in sample specimens to plastic surfaces. Consequently, misinterpretation of serological antibody assay data, which are largely influenced by the strong BG noise reaction and other false positive reactions, has led to uncertain conclusions and misunderstandings as discussed in detail [ 11 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Contribution of bacterial pathogens to evoking serological disease markers and aggravating disease activity in rheumatoid arthritis

Terato¹,

Waritani²,

Fukai³

et al. 2018

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Commensal bacteria and their pathogenic components in the gastrointestinal tract and oral cavity may play pathological roles in autoimmune diseases. To study the possible involvement of bacterial pathogens in autoimmune diseases, IgG and IgA antibodies against pathogenic components produced by three strains of commensal bacteria, Escherichia coli-lipopolysaccharide (E. coli-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and peptidoglycan polysaccharide (PG-PS) from Streptococcus pyogenes, were determined by an improved ELISA system for sera from two groups of patients with rheumatoid arthritis (RA), who met rapid radiographic progression (RRP) criteria and non-RRP, and compared to normal (NL) controls. Antibody responses to these bacterial pathogens are unique and consistent in individuals, and no fundamental difference was observed between RA and NL controls. Despite the similar antibody responses to pathogens, lower IgG or higher IgA and consequent higher IgA/IgG antibody ratio among the patients with RA related to disease marker levels and disease activity. Peculiarly, the IgA/IgG anti-Pg-LPS antibody ratio resulted from lower IgG and higher IgA antibody responses to Pg-LPS strongly correlated not only with rheumatoid factor (RF), but also correlated with erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and disease activity score of 28 joints with ESR (DAS28-ESR) in the RRP group. In contrast, the IgA/IgG anti-E. coli-LPS and anti-PG-PS antibody ratio correlated or tended to correlate with RF, ESR, CRP, and DAS28-ESR in the non-RRP group, whereas either the IgG or IgA anti-Pg-LPS antibody levels and consequent IgA/IgG anti-Pg-LPS antibody ratio did not correlate with any clinical marker levels in this group. Notably, anti-circular-citrullinated peptide (CCP) antibody levels, which did not correlate with either IgG or IgA antibody levels to any pathogens, did not correlate with severity of arthritis in both RRP and non-RRP. Taken together, we propose that multiple environmental pathogens, which overwhelm the host antibody defense function, contribute independently or concomitantly to evoking disease makers and aggravating disease activity, and affect disease outcomes.Trial registration: UMIN CTR UMIN000012200

show abstract

“…The diagnosis of aquaculture biota could be done in several ways, including isolation of the disease-causing agent and its morphological analysis, antibody detection resulting from infection with enzyme-linked immunosorbent assay or ELISA techniques, and gene detection of the disease-carrier agent with PCR [96][97][98]. One of the most widely applied and developed techniques today was PCR capable of amplifying DNA fragments in vitro.…”

Section: Dna Marker Methods To Evaluation and Diagnosismentioning

confidence: 99%

The Genetic Research Methods and its Role in Aquaculture on Indonesia

L¹

2018

IJOAC

View full text Add to dashboard Cite

Successful development of aquaculture is largely determined by the seed supply of both the quality and quantity of the seed. Seed quality is influenced by the quality of the mother (genetic factors) and environmental factors (water quality, food, and disease). Improved parent quality through the improvement of both qualitative and quantitative genetic traits that can be done through the selection of traits or characteristics of prospective mothers, as well as avoiding the occurrence of inbreeding that causes heritabilities decline. The purpose of writing to review the results of genetic studies that have been applied in the field of aquaculture in Indonesia. In this article, the study included the following: basic genetic concepts and theories, genetic research methods, and their roles and functions in the field of aquaculture.

show abstract

Preventing further misuse of the ELISA technique and misinterpretation of serological antibody assay data

Cited by 33 publications

References 7 publications

An ELISA protocol to improve the accuracy and reliability of serological antibody assays

An ELISA protocol to improve the accuracy and reliability of serological antibody assays

Contribution of bacterial pathogens to evoking serological disease markers and aggravating disease activity in rheumatoid arthritis

The Genetic Research Methods and its Role in Aquaculture on Indonesia

Contact Info

Product

Resources

About