Preventing multiple resistance above all: new insights for managing fungal adaptation

Abstract

Sustainable crop protection is crucial for food security, but is threatened by the adaptation of diverse, evolving pathogen population. Resistance can be managed by maximizing selection pressure diversity, by dose variation and the spatial and temporal combination of active ingredients. We explored the interplay between operational drivers for maximizing management strategy sustainability relative to the resistance status of fungal populations. We applied an experimental evolution approach to three artificial populations of an economically important wheat pathogen, Zymoseptoria tritici, differing in initial resistance status. We revealed that diversified selection pressure limited the selection of resistance in naive populations and those with low frequencies of single resistances. Increasing the number of modes of action delayed resistance development most effectively - ahead of increasing the number of fungicides, fungicide choice based on resistance risk and temporal variation in fungicide exposure - but favored generalism in the evolved populations. However, the prior presence of multiple resistance and its subsequent selection in populations overrode the effects of diversity in management strategies, incidentally invalidating any universal ranking. Initial resistance composition must therefore be considered specifically in sustainable resistance management, to address real-world field situation.