Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells <i>in Vivo</i>

Comenge, Joan; Fragueiro, Oihane; Sharkey, Jack; Taylor, Arthur; Held, Marie; Burton, Neal C.; Park, Brian Kevin; Wilm, Bettina; Murray, Patricia; Brust, Mathias; Lévy, Raphaël

doi:10.1021/acsnano.6b03246

Cited by 83 publications

(119 citation statements)

References 44 publications

Supporting

Mentioning

115

Contrasting

Order By: Relevance

“…This is in contrast to GNRs, which can have weaker signal due to plasmonic coupling when entrapped in subcellular vesicles or poor signal stability (Figure S4), which are not appropriate for sensitive and quantitative detections of injected cells. 14,15 …”

Section: Resultsmentioning

confidence: 99%

Photoacoustic Imaging of Human Mesenchymal Stem Cells Labeled with Prussian Blue–Poly(l-lysine) Nanocomplexes

et al. 2017

View full text Add to dashboard Cite

Acoustic imaging is affordable and accessible without ionizing radiation. Photoacoustic imaging increases the contrast of traditional ultrasound and can offer good spatial resolution when used at high frequencies with excellent temporal resolution. Prussian blue nanoparticles (PBNPs) are an emerging photoacoustic contrast agent with strong optical absorption in the near-infrared region. In this study, we developed a simple and efficient method to label human mesenchymal stem cells (hMSCs) with PBNPs and imaged them with photoacoustic imaging. First, PBNPs were synthesized by the reaction of FeCl3 with K4[Fe(CN)6] in the presence of citric acid and complexed with the cationic transfection agent poly-l-lysine (PLL). The PLL-coated PBNPs (PB-PLL nanocomplexes) have a maximum absorption peak at 715 nm and could efficiently label hMSCs. Cellular uptake of these nanocomplexes was studied using bright field, fluorescence, and transmission electron microscopy. The labeled stem cells were successfully differentiated into two downstream lineages of adipocytes and osteocytes, and they showed positive expression for surface markers of CD73, CD90, and CD105. No changes in viability or proliferation of the labeled cells were observed, and the secretome cytokine analysis indicated that the expression levels of 12 different proteins were not dysregulated by PBNP labeling. The optical properties of PBNPs were preserved postlabeling, suitable for the sensitive and quantitative detection of implanted cells. Labeled hMSCs exhibited strong photoacoustic contrast in vitro and in vivo when imaged at 730 nm, and the detection limit was 200 cells/µL in vivo. The photoacoustic signal increased as a function of cell concentration, indicating that the number of labeled cells can be quantified during and after cell transplantations. In hybrid ultrasound/photoacoustic imaging, this approach offers real-time and image-guided cellular injection even through an intact skull for brain intraparenchymal injections. Our labeling and imaging technique allowed the detection and monitoring of 5 × 104 mesenchymal stem cells in living mice over a period of 14 days.

show abstract

Section: Resultsmentioning

confidence: 99%

Photoacoustic Imaging of Human Mesenchymal Stem Cells Labeled with Prussian Blue–Poly(l-lysine) Nanocomplexes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…For instance, in the case of gold nanorods, cellular uptake into the cells via endocytosis and high concentration in endosomes may lead to diminished optoacoustic signal intensities. However, a steric hindrance provided by silica coating of the nanorods leads to improved optoacoustic sensitivity [314]. In this study, the enhanced sensitivity allowed for monitoring labelled stem cells implanted into mice over 15 days with high spatial resolution, albeit dilutions of the nanorods in the cell cluster are produced with each cell division.…”

Section: Applications Enabled By Optoacoustic Visualization Of Multmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

View full text Add to dashboard Cite

Visualization of dynamic functional and molecular events in an unperturbed in vivo environment is essential for understanding the complex biology of living organisms and of disease state and progression. To this end, optoacoustic (photoacoustic) sensing and imaging have demonstrated the exclusive capacity to maintain excellent optical contrast and high resolution in deep-tissue observations, far beyond the penetration limits of modern microscopy. Yet, the time domain is paramount for the observation and study of complex biological interactions that may be invisible in single snapshots of living systems. This review focuses on the recent advances in optoacoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches that enable new types of multiscale dynamic observations not attainable with other bio-imaging modalities. A wealth of investigated new research topics and clinical applications is further discussed, including imaging of large-scale brain activity patterns, volumetric visualization of moving organs and contrast agent kinetics, molecular imaging using targeted and genetically expressed labels, as well as three-dimensional handheld diagnostics of human subjects.

show abstract

“…Conversely, PAM approaches demonstrate detection of between 0.5 and 1 × 10 4 MSC labeled with Au nanocages . GNR can lose their plasmon resonance effects in vitro within the endosome of stem cells, and modification with silica can remove steric hindrance, prevent over‐confinement of particles within endosomes, and improve imaging signal . This information is summarized in Figure and Table .…”

Section: Cellular Prelabeling and Cell Imaging With Nanoparticlesmentioning

confidence: 99%

Engineering molecular imaging strategies for regenerative medicine

Willadsen

Chaise

Yarovoy

et al. 2018

Bioengineering & Transla Med

View full text Add to dashboard Cite

The reshaping of the world's aging population has created an urgent need for therapies for chronic diseases. Regenerative medicine offers a ray of hope, and its complex solutions include material, cellular, or tissue systems. We review basics of regenerative medicine/stem cells and describe how the field of molecular imaging, which is based on quantitative, noninvasive, imaging of biological events in living subjects, can be applied to regenerative medicine in order to interrogate tissues in innovative, informative, and personalized ways. We consider aspects of regenerative medicine for which molecular imaging will benefit. Next, genetic and nanoparticle‐based cell imaging strategies are discussed in detail, with modalities like magnetic resonance imaging, optical imaging (near infra‐red, bioluminescence), raman microscopy, and photoacoustic microscopy), ultrasound, computed tomography, single‐photon computed tomography, and positron emission tomography. We conclude with a discussion of “next generation” molecular imaging strategies, including imaging host tissues prior to cell/tissue transplantation.

show abstract

Preventing Plasmon Coupling between Gold Nanorods Improves the Sensitivity of Photoacoustic Detection of Labeled Stem Cells in Vivo

Cited by 83 publications

References 44 publications

Photoacoustic Imaging of Human Mesenchymal Stem Cells Labeled with Prussian Blue–Poly(l-lysine) Nanocomplexes

Photoacoustic Imaging of Human Mesenchymal Stem Cells Labeled with Prussian Blue–Poly(l-lysine) Nanocomplexes

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

Engineering molecular imaging strategies for regenerative medicine

Contact Info

Product

Resources

About