Objective: To characterize the symptoms of musculoskeletal disorders (MSDs) and their related factors in a group of orthodontists in the city of Medellín (Colombia). Methods: A cross-sectional study was conducted in a sample of 100 orthodontists. A survey provided sociodemographic and work-related information. The MSDs were recorded through the Nordic Questionnaire (Kourinka et al., 1987). Descriptive and bivariate analyses were carried out by determining the prevalence of MSDs symptoms by anatomical zone and selected variables. Results: Prevalence of the MSD was 81% for males (m) and 88% for females (f). The anatomical regions were: neck (m: 44%, f: 59%), shoulders (m: 44%, f: 48%), lower backlumbar zone (m: 63%, f: 51%), elbow-forearm (m: 15%, f: 15%) and, wrist-hand (m: 32%, f: 51%). The frequency of symptoms was higher in males > 41 years, with more than a work contract or females worked > 40 hours per week, and in orthodontists (males-females) who reported higher work-related dissatisfaction levels. Conclusions: A high prevalence of MSDs was found among orthodontists, with differences in the frequency of symptoms related to sociodemographic and work-related factors. Preventive strategies related to health and work safety would be advisable considering the particular needs of this dentists' group.