Prevention of Adhesion and Promotion of Pseudoislets Formation from a β‐Cell Line by Fluorocarbon Emulsions

Domínguez, Margarita Sánchez; Maillard, Élisa; Krafft, Marie Pierre; Sigrist, S.; Belcourt, A.

doi:10.1002/cbic.200600056

Cited by 25 publications

(18 citation statements)

References 28 publications

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“…Use of PFCOC emulsion prevented the adhesion of established pancreatic beta cell lines and the oxygen-carrying capacity of PFCOC emulsion may also help to prevent the period of hypoxia exposure, which is known to contribute to the failure of post-transplant survival [83]. Glucose is the major source of energy for the cells for special tasks such as protein synthesis, nucleic acid replication and other intracellular processes [84].…”

Section: Discussionmentioning

confidence: 99%

Functional, Metabolic and Morphologic Results of Ex Vivo Donor Lung Perfusion with a Perfluorocarbon-Based Oxygen Carrier Nanoemulsion in a Large Animal Transplantation Model

İnci

Arni

Çıtak

et al. 2020

Cells

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Background: Ex vivo lung perfusion (EVLP) is a technology that allows the re-evaluation of questionable donor lung before implantation and it has the potential to repair injured donor lungs that are otherwise unsuitable for transplantation. We hypothesized that perfluorocarbon-based oxygen carrier, a novel reconditioning strategy instilled during EVLP would improve graft function. Methods: We utilized perfluorocarbon-based oxygen carrier (PFCOC) during EVLP to recondition and improve lung graft function in a pig model of EVLP and lung transplantation. Lungs were retrieved and stored for 24 h at 4 °C. EVLP was done for 6 h with or without PFCOC. In the transplantation groups, left lung transplantation was done after EVLP with or without PFCOC. Allograft function was assessed by means of pulmonary gas exchange, lung mechanics and vascular pressures, histology and transmission electron microscopy (TEM). Results: In the EVLP only groups, physiological and biochemical markers during the 6-h perfusion period were comparable. However, perfusate lactate potassium levels were lower and ATP levels were higher in the PFCOC group. Radiologic assessment revealed significantly more lung infiltrates in the controls than in the PFCOC group (p = 0.04). In transplantation groups, perfusate glucose consumption was higher in the control group. Lactate levels were significantly lower in the PFCOC group (p = 0.02). Perfusate flavin mononucleotide (FMN) was significantly higher in the controls (p = 0.008). Post-transplant gas exchange was significantly better during the 4-h reperfusion period in the PFCOC group (p = 0.01). Plasma IL-8 and IL-12 levels were significantly lower in the PFCOC group (p = 0.01, p = 0.03, respectively). ATP lung tissue levels at the end of the transplantation were higher and myeloperoxidase (MPO) levels in lung tissue were lower in the PFCOC group compared to the control group. In the PFCOC group, TEM showed better tissue preservation and cellular viability. Conclusion: PFCOC application is safe during EVLP in lungs preserved 24 h at 4 °C. Although this strategy did not significantly affect the EVLP physiology, metabolic markers of the donor quality such as lactate production, glucose consumption, neutrophil infiltration and preservation of mitochondrial function were better in the PFCOC group. Following transplantation, PFCOC resulted in better graft function and TEM showed better tissue preservation, cellular viability and improved gas transport.

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Section: Discussionmentioning

confidence: 99%

Functional, Metabolic and Morphologic Results of Ex Vivo Donor Lung Perfusion with a Perfluorocarbon-Based Oxygen Carrier Nanoemulsion in a Large Animal Transplantation Model

İnci

Arni

Çıtak

et al. 2020

Cells

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“…A diblock-stabilized FC emulsion was found to prevent the adhesion of certain β-cell lines to cell culture plastic and promoted the formation of pseudo-islets capable of insulin secretion [96].…”

Section: Fluorocarbon/hydrocarbon Diblock Amphiphilesmentioning

confidence: 99%

“…New experimental FC-in-water emulsions are being investigated for this purpose that use a combination of phospholipids and a FnHm diblock (e.g. F6H10) as the emulsifier [96]. The diblock provides a substantial increase in emulsion stability, allowing long term storage, as well as controllable droplet sizes and narrow size distributions.…”

Section: Fluorocarbon/hydrocarbon Diblock Amphiphilesmentioning

confidence: 99%

Highly fluorinated amphiphilic molecules and self-assemblies with biomedical potential

Riess¹

2009

Current Opinion in Colloid & Interface Science

125

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“…A PFC emulsion was found to prevent the adhesion of certain b-cell lines to cell culture plastic and promoted the formation of pseudoislets capable of insulin secretion. 67 The development of appropriate drug delivery technology is gaining importance in disease management. Each drug requires its specific delivery system that optimizes efficacy and minimizes side effects (e.g., anticancer agents) through controlled release and, optimally, targeting.…”

Section: Perfluorocarbons As Drugs and Drug-delivery Systemsmentioning

confidence: 99%

Perfluorocarbons: Life sciences and biomedical uses Dedicated to the memory of Professor Guy Ourisson, a true RENAISSANCE man.

Krafft

Riess

2007

J. Polym. Sci. A Polym. Chem.

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188

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ABSTRACT:Perfluorocarbons are primarily characterized by outstanding chemical and biological inertness, and intense hydrophobic and lipophobic effects. The latter effects provide a powerful noncovalent, labile binding interaction that can promote selective selfassembly. Perfluoro compounds do not mimic nature, yet they can offer abiotic building blocks for the de novo design of functional biopolymers and alternative solutions to physiologically vital issues. They offer new tags useful for molecular recognition, selective sorting, and templated binding (e.g., selective peptide and nucleic acid pairing). They also stabilize membranes and provide microand nanocompartmented fluorous environments.Perfluorocarbons provide inert, apolar carrier fluids for lab-on-a-chip experiments and assays using microfluidic technologies. Low water solubility, combined with high vapor pressure, allows stabilization of injectable microbubbles that serve as contrast agents for diagnostic ultrasound imaging. High gas solubilities are the basis for an abiotic means for intravascular oxygen delivery. Other biomedical applications of fluorocarbons include lung surfactant replacement and ophthalmologic aids. Diverse colloids with fluorocarbon phases and/or shells are being investigated for molecular imaging using ultrasound or magnetic resonance, and for targeted drug delivery. Highly fluorinated polymers provide a range of inert materials (e.g., fluorosilicons, expanded polytetrafluoroethylene) for contact lenses, reconstructive surgery (e.g., vascular grafts), and other devices.

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Prevention of Adhesion and Promotion of Pseudoislets Formation from a β‐Cell Line by Fluorocarbon Emulsions

Cited by 25 publications

References 28 publications

Functional, Metabolic and Morphologic Results of Ex Vivo Donor Lung Perfusion with a Perfluorocarbon-Based Oxygen Carrier Nanoemulsion in a Large Animal Transplantation Model

Functional, Metabolic and Morphologic Results of Ex Vivo Donor Lung Perfusion with a Perfluorocarbon-Based Oxygen Carrier Nanoemulsion in a Large Animal Transplantation Model

Highly fluorinated amphiphilic molecules and self-assemblies with biomedical potential

Perfluorocarbons: Life sciences and biomedical uses Dedicated to the memory of Professor Guy Ourisson, a true RENAISSANCE man.

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