Prevention of collagen-induced arthritis in DBA/1 mice by oral administration of AZ-9, a bacterial polysaccharide from Klebsiella oxytoca

Sugihara, Ryosuke; Yoshimura, Masayasu; Mori, Masaharu; Kanayama, Naoki; Hara, Masaki; Ohmori, Hitoshi

doi:10.1016/s0162-3109(00)00247-2

Cited by 18 publications

(15 citation statements)

References 33 publications

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“…Indeed, cEPS strongly reduced the serum level of total collagen-specific IgG and of both measured subclasses of IgG, namely IgG1 and IgG2a [11]. The inhibitory effect of EPS on the humoral response was also confirmed by others [13].…”

Section: Introductionsupporting

confidence: 62%

Experimental immunology Immunosuppressive effect of systemic administration of Lactobacillus rhamnosus KL37C-derived exopolysaccharide on the OVA-specific humoral response

Ciszek-Lenda¹,

Nowak²,

Śróttek³

et al. 2012

cejoi

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Section: Introductionsupporting

confidence: 62%

Experimental immunology Immunosuppressive effect of systemic administration of Lactobacillus rhamnosus KL37C-derived exopolysaccharide on the OVA-specific humoral response

Ciszek-Lenda¹,

Nowak²,

Śróttek³

et al. 2012

cejoi

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“…Treatment with microbial products has been shown to prevent CIA (33) or reverse developing arthritis (34). In a similar fashion, we have shown that a single dose of a live bacterial vaccine, Salmonella -CFA/I, when given to DBA/1 mice 7 days prior CIA induction, prevents development of disease (10).…”

Section: Discussionmentioning

confidence: 99%

Segregated Regulatory CD39+CD4+ T Cell Function: TGF-β–Producing Foxp3− and IL-10–Producing Foxp3+ Cells Are Interdependent for Protection against Collagen-Induced Arthritis

Kochetkova

Thornburg

Callis

et al. 2011

The Journal of Immunology

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Oral immunization with a Salmonella vaccine vector expressing enterotoxigenic E. coli colonization factor antigen I (CFA/I) can protect against collagen-induced arthritis (CIA) by dampening IL-17 and IFN-γ via enhanced IL-4, IL-10, and TGF-β. To identify the responsible regulatory CD4+ T cells making the host refractory to CIA, Salmonella-CFA/I induced CD39+CD4+ T cells with enhanced apyrase activity relative to Salmonella vector-immunized mice. Adoptive transfer of vaccine-induced CD39+CD4+ T cells into CIA mice conferred complete protection, while CD39−CD4+ T cells did not. Subsequent analysis of vaccinated FoxP3-GFP mice revealed the CD39+ T cells were composed of FoxP3-GFP− and FoxP3-GFP+ subpopulations. Although each adoptively transferred Salmonella-CFA/I-induced FoxP3− and FoxP3+CD39+CD4+ T cells could protect against CIA, each subset was not as efficacious as total CD39+CD4+ T cells, suggesting their interdependence for optimal protection. Cytokine analysis revealed FoxP3− CD39+CD4+ T cells produced TGF-β, and FoxP3+CD39+CD4+ T cells produced IL-10, showing a segregation of function. Moreover, donor FoxP3-GFP− CD4+ T cells converted to FoxP3-GFP+ CD39+CD4+ T cells in the recipients, showing plasticity of these regulatory T cells. TGF-β was found to be essential for protection since in vivo TGF-β neutralization reversed activation of cAMP-response element-binding protein (CREB) and reduced the development of CD39+CD4+ T cells. Thus, CD39 apyrase-expressing CD4+ T cells stimulated by Salmonella-CFA/I are composed of TGF-β-producing FoxP3− CD39+CD4+ T cells and support the stimulation of IL-10-producing FoxP3+ CD39+CD4+ T cells.

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“…l-Cys amino acid, which has a wide spectrum of microbial activity and well-known safety [10][11][12], was for the first time functionalized at its lowest concentration -1% over the weight of the fabric (wof). In previous works the minimum amount used with efficiency of microbial killing was 5% owf, in combination with either wool [23], or cotton [10,12]. This l-Cys fall is understandable, in that recently new approaches relying on removal of lipids from the surface of fibers have exposed their surface with a variety of reactive moieties.…”

Section: Discussionmentioning

confidence: 99%

“…Although it is stated that the itching of AD is exacerbated by spiky fibers of wool and by the wild texture of cotton [8,9], silk, on the other hand, with its extremely smooth and its comfortable properties reduces these effects and therefore has been increasingly researched for applications related to atopic dermatitis (AD). Furthermore, Sugihara et al [10] postulated that wounds treated with a silk dressing healed 7 days faster, by promoting the enhanced synthesis of collagen and epithelialization [10].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and antioxidant surface modification toward a new silk-fibroin (SF)-l-Cysteine material for skin disease management

Nogueira

Granadeiro

Mouro

et al. 2016

Applied Surface Science

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A novel dressing material-silk fibroin fabric (SF)-l-Cysteine (l-Cys)-is here developed to be used as standard treatment for atopic dermatitis (AD), which combines comfort, thermic, and tensile strength properties of silk materials with antioxidant and antimicrobial effects of l-Cys. A careful understanding about the linking strategies is needed in order not to compromise the bioavailability of l-Cys and deplenish its bioactivity. Durability was also addressed through washing cycles and compared with hospital requirements, according to international Standard EN ISO 105-C06:2010. The present research also analyze the interactions between Staphylococcus aureus and SF-l-Cys under simulating conditions of AD and demonstrated the effectiveness of a double covalent grafting, with the importance of SF tyrosine (Tyr) covalent linkage with l-Cys (SF-g-l-Cys/Tyr-g-l-Cys) even after several washing cycles, twenty five, whereas for a disposable application a single covalent mechanism of grafting l-Cys proved to be sufficient (SF-g-l-Cys). Results showed effective antimicrobial activities exhibiting higher inhibition ratios of 98.65% for SF-g-l-Cys after 5 washing cycles, whereas 97.55% for SF-g-l-Cys/Tyr-g-l-Cys after 25 washing cycles, both at pH 9.5 grafting strategy. Furthermore, it is also reported a non-protumoral effect of l-Cys. A new advance is herein achieved at the world of medical antimicrobial textiles tailored to address wound moisture environment and exudate self-cleaning, which may open novel applications as complementary therapy for AD disease.

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Prevention of collagen-induced arthritis in DBA/1 mice by oral administration of AZ-9, a bacterial polysaccharide from Klebsiella oxytoca

Cited by 18 publications

References 33 publications

Experimental immunology Immunosuppressive effect of systemic administration of Lactobacillus rhamnosus KL37C-derived exopolysaccharide on the OVA-specific humoral response

Experimental immunology Immunosuppressive effect of systemic administration of Lactobacillus rhamnosus KL37C-derived exopolysaccharide on the OVA-specific humoral response

Segregated Regulatory CD39+CD4+ T Cell Function: TGF-β–Producing Foxp3− and IL-10–Producing Foxp3+ Cells Are Interdependent for Protection against Collagen-Induced Arthritis

Antimicrobial and antioxidant surface modification toward a new silk-fibroin (SF)-l-Cysteine material for skin disease management

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