Prevention of disorders in the mucous membrane of the digestive tract of female rats with estrogen, protein and calcium deficiency

Abstract

Purpose. Substantiation of the preventive efficacy of a complex consisting of quercetin, calcium citrate, vitamins, macro- and microelements in relation to the biochemical parameters of the mucous membranes of the digestive tract of rats with oophorectomy and insufficient protein and calcium in the diet. Materials and methods. The study was conducted on 24 female rats of herd breeding: in 16 animals, the state of estrogen deficiency was modeled by ovariectomy followed by their maintenance on an inadequate diet, half of which were injected with a prophylactic complex of vitamins and minerals. After 4 months, the rats were taken out of the experiment. In the homogenates of the mucous membranes of the oral cavity, stomach, small and large intestine, markers of inflammation (activity of elastase, acid phosphatase and the content of malonic dialdehyde) and the index of bacterial contamination (activity of urease) were determined. Results. As a result of the study, the development of inflammation was established in the mucous membranes of the digestive tract of rats with hypoestrogenism and when receiving a diet deficient in protein and calcium. The development of inflammation was accompanied by an increase in the activity of acid phosphatase (in the oral cavity – by 16.5 %, in the stomach – by 23.3 %, in the small intestine – by 27.0 %, in the large intestine – by 29.6 %), elastase ( in the oral cavity – by 9.2 %, in the stomach – by 52.6 %, in the small intestine – by 93.6 %, in the large intestine – by 28.7 % and the content of malondialdehyde (in the oral cavity – by 25.8 %, in the stomach – by 40.7 %, in the small and large intestine – by 26.4 %, in the large intestine – by 35.7 %) relative to the control group. In addition, an increase in urease activity was registered in the mucous membranes of the digestive tract of rats with pathology, which means an increase in the contamination of opportunistic microorganisms on the mucous membranes. The introduction of the prophylactic complex to rats prevented an increase in inflammation and dysbiosis in the mucous membranes of the digestive tract of animals, which was caused by pathology modeling. Findings. Anti-inflammatory and anti-dysbiotic properties of the prophylactic complex of vitamins and minerals based on quercetin and calcium citrate from Black Sea oyster shells allow us to recommend this composition of preparations for a deeper study in order to create an alternative to hormone replacement therapy for hypoestrogenism with alimentary deficiency of protein and calcium.