Prevention of electrode fouling in high field sterilization

Bushnell, Alison; Clark, Richard W.; Dunn, Joseph; Lloyd, S.W.

doi:10.1016/s0924-2244(00)89172-4

Cited by 2 publications

(4 citation statements)

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“…Electric field pulses can be applied for electroporation, gene transfer, electrofusion, electrorotation, dielectrophoresis and the killing of cells (30,(37)(38)(39)(40)(41)(42)(43). Generally, extremely high electric field strengths and solutions of high specific resistance are necessary to perform these electric manipulations.…”

Section: Discussionmentioning

confidence: 99%

“…Electrochemical and electrophoretic effects may have contributed to cell disintegration (18,51). However, the traces of DNA and protein that were found to be depositioned on the electrodes could easily be rinsed off.…”

Section: Discussionmentioning

confidence: 99%

“…The cell disintegration chamber was thermostated by a Julabo F 10 thermostat from Julabo Labortechnik GmbH (Seelbach, Germany). The PG was built as outlined previously (18) to prevent electrochemical and electrophoretic effects. It was equipped with a capacitance (C) of ∼1.67 nF.…”

Section: Methodsmentioning

confidence: 99%

“…Although a system devised to prevent electrochemical and electrophoretic effects (18) was used, we observed electrophoresis after the application of 18 000 electric field pulses and a treatment time of 60 min, as detected by migration of bromophenolblue [0.25% (w/v)] in a 1% agarose gel poured into the RC (data not shown). Compared to the cell suspensions measured previously, the electric current was only half as high.…”

Section: Electrochemical Side Effectsmentioning

confidence: 95%

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Amplifiable DNA from Gram-negative and Gram-positive bacteria by a low strength pulsed electric field method

Vitzthum

Geiger²,

Bisswanger³

et al. 2000

Nucleic Acids Research

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An efficient electric field-based procedure for cell disruption and DNA isolation is described. Isoosmotic suspensions of Gram-negative and Gram-positive bacteria were treated with pulsed electric fields of <60 V/cm. Pulses had an exponential decay waveform with a time constant of 3.4 micros. DNA yield was linearly dependent on time or pulse number, with several thousand pulses needed. Electrochemical side-effects and electrophoresis were minimal. The lysates contained non-fragmented DNA which was readily amplifiable by PCR. As the method was not limited to samples of high specific resistance, it should be applicable to physiological fluids and be useful for genomic and DNA diagnostic applications.

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