Prevention of Gordonia and Nocardia Stabilized Foam Formation by Using Bacteriophage GTE7

Petrovski, Steve; Seviour, Robert J.; Tillett, Daniel

doi:10.1128/aem.05692-11

Cited by 68 publications

(56 citation statements)

References 22 publications

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“…A similar level of stringent strain specificity has been observed in other actinophages (15)(16)(17)(18)(19)(20)(21)27). Possible explanations include an absence of a compatible receptor site in the resistant host cell, absence of appropriate molecular machinery to support infection, or possession of phage resistance systems like clustered regularly interspersed repeat (CRISPR) systems, restriction modification (RM) systems, and abortive infection (Abi) systems (28).…”

Section: Resultsmentioning

confidence: 89%

“…Pellets were resuspended in 8 M urea to a final volume of 100 l prior to analyses at the Mass Spectroscopy and Proteomics Facility at the La Trobe Institute of Molecular Sciences (Melbourne, Australia). Peptides reconstituted in 0.1% formic acid and 2% acetonitrile (buffer A) were loaded onto a trap column (C 18 PepMap, 300-m inner diameter [i.d. ], 2-cm trapping column [Thermo-Fisher Scientific, Scoresby, Australia]) at 5 l/min for 6 min and washed for 6 min before switching the precolumn in line with the analytical column (Vydac MS C 18 , 75-m i.d.…”

Section: Methodsmentioning

confidence: 99%

“…Thomas et al (14) proposed that a phage therapy biocontrol approach might be an attractive option for treating this global operational problem and successfully isolated 17 phages infective for foaming mycolata from Australian wastewater treatment plants. More mycolata phages have been isolated and described since then (15)(16)(17)(18)(19)(20)(21). Among them is the TPA2 phage, infective for members of the genus Tsukamurella (15).…”

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confidence: 99%

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Three of a Kind: Genetically Similar Tsukamurella Phages TIN2, TIN3, and TIN4

Dyson

Tucci

Seviour

et al. 2015

Appl Environ Microbiol

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bThree Tsukamurella phages, TIN2, TIN3, and TIN4, were isolated from activated sludge treatment plants located in Victoria, Australia, using conventional enrichment techniques. Illumina and 454 whole-genome sequencing of these Siphoviridae viruses revealed that they had similar genome sequences, ranging in size between 76,268 bp and 76,964 bp. All three phages shared 74% nucleotide sequence identity to the previously described Gordonia phage GTE7. Genome sequencing suggested that phage TIN3 had suffered a mutation in one of its lysis genes compared to the sequence of phage TIN4, to which it is genetically very similar. Mass spectroscopy data showed the unusual presence of a virion structural gene in the DNA replication module of phage TIN4, disrupting the characteristic modular genome architecture of Siphoviridae phages. All three phages appeared highly virulent on strains of Tsukamurella inchonensis and Tsukamurella paurometabola. The genus Tsukamurella contains Gram-positive organisms in the Corynebacteriales (1) and currently includes 11 species (2). Members of this genus are strictly aerobic and weakly/variably acid-fast, nonmotile, non-spore-forming organisms whose cell envelopes contain long-chain unsaturated mycolic acids (3-8).Together with other mycolic acid-producing genera, including Mycobacterium, Millisia, Nocardia, Rhodococcus, Williamsia, Rhodococcus, and Gordonia, they are often referred to collectively as the mycolata, a group of organisms whose mycolic acids render their cells highly hydrophobic (9, 10).The high hydrophobicity and the biosurfactants produced by the mycolata implicate them as stabilizing agents of foams in activated sludge plants (9, 11). Several Tsukamurella species including T. spumae (12), T. sunchonensis (13), and T. pseudospumae (12) have been isolated from these foams. Stable foams complicate sludge management, have a negative impact on plant esthetics, and increase maintenance costs (9).Several opportunistic Tsukamurella pathogens are known to exist, thus posing a potential health hazard to plant operators from aerosol foam dispersal (9). Thomas et al. (14) proposed that a phage therapy biocontrol approach might be an attractive option for treating this global operational problem and successfully isolated 17 phages infective for foaming mycolata from Australian wastewater treatment plants. More mycolata phages have been isolated and described since then (15-21). Among them is the TPA2 phage, infective for members of the genus Tsukamurella (15).As of March 2015, genomes of 228 phages infective for members of the closely related genus Mycobacterium have been sequenced. To better understand their genomics, Hatfull et al. (22) devised a clustering system that claimed to reveal insights into their evolutionary interrelationships. In this system, TPA2 phage would be placed into their cluster B as its genome organization is similar to that of the Mycobacterium phage Rosebush (15). Despite this similarity between TPA2 and Mycobacterium phages, little else is known about the exte...

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Section: Resultsmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Three of a Kind: Genetically Similar Tsukamurella Phages TIN2, TIN3, and TIN4

Dyson

Tucci

Seviour

et al. 2015

Appl Environ Microbiol

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“…2 Bacteriophages were grown in lab and a sufficient amount of bacteriophages were applied to their respective hosts. The data showed CFU/ml decreased markedly in the presence of NOC1, NOC2, and NOC3 when collectively applied to the lab scale reactor.…”

Section: Applicability Of Phage To Lab Scale Reactormentioning

confidence: 99%

“…1,2 In the previous study, we had isolated and tested specific bacteriophages against Nocardioforms -NOC1, NOC2 and NOC3 and showed that they can be used effectively to curb foaming. Their host range was determined and genome sequences studied.…”

Section: Introductionmentioning

confidence: 99%

Novel application of bacteriophage for controlling foaming in wastewater treatment plant- an eco-friendly approach

et al. 2016

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This addendum to "Novel application of bacteriophage for controlling foaming in wastewater treatment plant-an eco-friendly approach " includes characteristics of the phages NOC1, NOC2 and NOC3 not discussed in the previous paper. The phage adsorption and host interaction properties, their sensitivity to pH and temperature are inferred. NOC2 is seen to be more temperature resistant while others are not. All the phages show pH sensitivity. There is a variance observed in the behavior of these phages. Also, applicability of the phage based system to large scale reactors is studied and discussed here.

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