2022
DOI: 10.21203/rs.3.rs-1819359/v1
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Prevention of horizontal transfer of laboratory plasmids to environmental bacteria: how to select disinfection methods that are also effective in destroying DNA

Abstract: Routine work of any molecular biology laboratory includes the daily use of microorganisms, including non-pathogenic E coli strains, transformed with a variety of plasmids expressing at least one antibiotic resistance gene (ARG). To check the efficacy of neutralization methods, bacteria isolated from wastewater from laboratories and research institutes were identified using the 16S ribosomal RNA sequencing and they were tested for the presence of an origin of replication and several ARGs frequently found in lab… Show more

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