2000
DOI: 10.1007/s001470050392
|View full text |Cite
|
Sign up to set email alerts
|

Prevention of hyperacute xenograft rejection in orthotopic xenotransplantation of pig hearts into baboons using immunoadsorption of antibodies and complement factors

Abstract: To prevent hyperacute xenograft rejection (HXR) caused by preformed natural antibodies (XNAb) after orthotopic heart xenotransplantation (oXHTx) of landrace pig hearts into baboons, we used immunoadsorption of immunoglobulins IgG, IgM and IgA and complement with the reusable Ig-Therasorb column. In addition to functional data, tissue was sampled for histological, immunohistochemical and electron microscopical analysis. We performed three oXHTx of landrace pig hearts to baboons using extracorporeal circulation … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2

Citation Types

2
2
0

Year Published

2001
2001
2022
2022

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 9 publications
(4 citation statements)
references
References 20 publications
2
2
0
Order By: Relevance
“…Our results show that most, but not all, of the endothelial cells from Tg piglets demonstrated the protective effect of the complement system against attack, albeit with different efficiencies. Our results were also consistent with previous studies in showing that the expression of CRPs and the reduction of α-Gal in transgenic endothelial cells directly affects protection against complement-mediated cytolysis [31], [42], [43], [44]. Additionally, SUVEC provides a useful tissue model for performing functional analyses for xenograft organs with minimal sacrifice.…”
Section: Discussionsupporting
confidence: 92%
“…Our results show that most, but not all, of the endothelial cells from Tg piglets demonstrated the protective effect of the complement system against attack, albeit with different efficiencies. Our results were also consistent with previous studies in showing that the expression of CRPs and the reduction of α-Gal in transgenic endothelial cells directly affects protection against complement-mediated cytolysis [31], [42], [43], [44]. Additionally, SUVEC provides a useful tissue model for performing functional analyses for xenograft organs with minimal sacrifice.…”
Section: Discussionsupporting
confidence: 92%
“…In the presented study the CO of the xenogeneic porcine heart after orthotopic transplantation is in good agreement with previous results from a smaller study [23,24]. The 21% increase in CO after orthotopic transplantation was caused by a pronounced increase (87%) in heart rate, whereas stroke volume showed a tendency towards a reduction (−21%; not significant).…”
Section: Discussionsupporting
confidence: 91%
“…Because of the shortage of transplant organs, xenotransplantation has become an active research area. Although hyperacute rejection of xenotransplants can be prevented by neutralization of Xab and/or complement factors, acute graft rejection is still a major obstacle to long‐term survival of xenografts (6). Inflammation and apoptosis of graft endothelial cells play an important role in acute vascular rejection.…”
mentioning
confidence: 99%
“…A growing body of evidence suggests that the expression of HO‐1 can enhance the survival of concordant transplanted organs (8). Presumably, the protection is associated with the anti‐apoptotic, anti‐inflammatory and anti‐proliferative effects of HO‐1 or its byproducts (6, 9–12). However, the effect of HO‐1 in discordant xenograft remains unclear.…”
mentioning
confidence: 99%