Prevention of inorganic arsenic induced squamous cell carcinoma of the skin in Swiss albino mice by black tea through epigenetic modulation

Ghosh, Archismaan; Lahiri, Ansuman; Mukherjee, Sutapa; Roy, Madhumita; Datta, Amitava

doi:10.1016/j.heliyon.2022.e10341

Cited by 7 publications

(8 citation statements)

References 56 publications

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“…3) Preparation of BTE BTE administered to mice was prepared according to the standard laboratory protocol [8].…”

Section: ) Animal Treatmentmentioning

confidence: 99%

“…IHC was performed on paraffin embedded formalin fixed tissues [8]. Primary antibodies were added in the ratio 1:500 and incubated overnight at 4°C.…”

Section: ) Immunohistochemistry (Ihc)mentioning

confidence: 99%

“…These results indicate that BTE inhibits iAs induced skin carcinogenesis. This data has already been published from our laboratory [8]. To understand the preventive mechanism of BTE in iAs-induced SCC, detailed investigation was carried out.…”

Section: Development Of Scc Of the Skin In Swiss Albino Mice And Its ...mentioning

confidence: 99%

“…Black tea, one of the most popular beverages, exhibits pronounced antioxidative properties [28] by inhibiting/scavenging free radicals. Report from our laboratory showed that black tea extract (BTE) effectively quenched iAs-induced ROS production [7], and mitigated epigenetic modulations, preventing carcinogenesis in Swiss albino mice [8]. BTE was found to inhibit iAs-induced EMT in skin keratinocyte (HaCaT) cells, thus exhibiting its chemopreventive potential in an in vitro model [29].…”

Section: Introductionmentioning

confidence: 99%

“…Swiss albino mice chronically exposed to iAs encountered excess generation of reactive oxygen species (ROS), resulting in DNA, lipid and protein damage, induction of inflammatory cytokine production, and impairment of repair enzymes [7], as well as aberrant epigenetic modulations [8]. Excess ROS generation is linked to induction of cancer and its progression.…”

Section: Introductionmentioning

confidence: 99%

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Black Tea Extract, via Modulation of TGF-β Pathway, Prevents Inorganic Arsenic-induced Development of Squamous Cell Carcinoma of the Skin in Swiss Albino Mice

Ghosh

Roy

2023

J Cancer Prev

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Chronic exposure to inorganic arsenic (iAs) elevates reactive oxygen species (ROS) generation and up-regulates TGF-β signalling. This promotes induction of epithelial to mesenchymal transition (EMT) and causes the development of squamous cell carcinoma (SCC) of skin. Black tea is a popular beverage worldwide and an effective antioxidant. Chemopreventive potential of black tea extract (BTE) against iAs induced carcinogenicity has been explored here. The study aims to investigate the role of BTE in prevention of iAs-induced SCC of skin in Swiss albino mice via the modulation of TGF-β signalling and EMT. Mice were divided into (1) control, (2) iAs, (3) iAs+BTE, and (4) BTE groups and were administered iAs and BTE alone, or in combination for 330 days. Histological studies were performed to assess development of SCC. ROS generation was estimated by flowcytometry. Expression of TGF-β and downstream proteins belonging to suppressor of mothers against decapentaplegic (Smad), phosphoinositide-3-kinase (PI3K)-protein kinase B (AKT) and mitogen-activated protein kinase (MAPK) pathways was assessed by immunoblotting. Expression of EMT markers was evaluated by immunoblotting, immunohistochemistry and semi-quantitative reverse transcriptase-PCR. After 330 days of iAs treatment, development of invasive SCC of skin probably due to excess ROS generation, elevation of TGF-β, downregulation of the Smad pathway, upregulation of PI3K-AKT and MAPK signalling molecules and induction of EMT was observed. All these modulations were found to be reversed by BTE, which inhibits iAs induced SCC of skin by quenching excess ROS, promoting Smad mediated TGF-β signalling, downregulating signalling intermediates of PI3K-AKT and MAPK pathways and inhibiting EMT.

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“…3) Preparation of BTE BTE administered to mice was prepared according to the standard laboratory protocol [8].…”

Section: ) Animal Treatmentmentioning

confidence: 99%

“…IHC was performed on paraffin embedded formalin fixed tissues [8]. Primary antibodies were added in the ratio 1:500 and incubated overnight at 4°C.…”

Section: ) Immunohistochemistry (Ihc)mentioning

confidence: 99%

Section: Development Of Scc Of the Skin In Swiss Albino Mice And Its ...mentioning

confidence: 99%