Prevention of intestinal amebiasis by vaccination with the Entamoeba histolytica Gal/GalNac lectin

Houpt, Eric R.; Barroso, Lisa; Lockhart, Lauren A.; Wright, Rhonda L.; Cramer, Carole L.; Lyerly, David M.; Petri, William A.

doi:10.1016/j.vaccine.2003.09.003

Cited by 94 publications

(85 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LecA recombinant antigen, containing the carbohydrate recognition domain for galactose and N-acetyl-D-galactosamine complex from E. histolytica, was expressed with six histidines at the amino terminus as previously described. 20 All other antigens were GST-linked. Recombinant antigens from C. parvum, Cry17, and Cry27, have been previously described.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Moss

Priest

Hamlin³

et al. 2014

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

Abstract. Haitian children were monitored longitudinally in a filariasis study. Included were stool samples examined for Giardia intestinalis and Entamoeba histolytica cysts, and serum specimens analyzed for immunoglobulin G (IgG) responses to eight recombinant antigens from G. intestinalis (variant-specific surface protein [VSP1-VSP5]), E. histolytica (lectin adhesion molecule [LecA]), and Cryptosporidium parvum (17-and 27-kDa) using a multiplex bead assay. The IgG responses to VSP antigens peaked at 2 years of age and then diminished and were significantly lower (P 0.002) in children 4.5 years than in children 4.5 years. The IgG responses to Cryptosporidium tended to increase with age. The IgG responses to LecA and VSP antigens and the prevalence of stools positive for cysts were significantly higher (P 0.037 and P 0.035, respectively) in the rainy season than in the dry season. The multiplex bead assay provides a powerful tool for analyzing serologic responses to multiple pathogens.

show abstract

Section: Methodsmentioning

confidence: 99%

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Moss

Priest

Hamlin³

et al. 2014

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

show abstract

“…The purified native Gal/GalNAc lectin has been demonstrated to be efficacious to protect gerbils and mice from amebic liver abscess (66) and intestinal infection (67), respectively. Protection from amebic colitis was associated with a positive fecal anti-lectin IgA response (67). Vaccination with the recombinant lectin fragment (LecA) elicited a substantial IgA response to the antigen, but the degree of protection was weaker compared to those vaccinated with native lectin (67).…”

Section: Humoral Immune Response To E Histolyticamentioning

confidence: 99%

Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies

Guo

Houpt

Petri

2007

Current Opinion in Immunology

Self Cite

View full text Add to dashboard Cite

The host-parasite relationship is based on a series of interplays between host defense mechanisms and parasite survival strategies. Progress has been made in understanding the role of host immune response in amebiasis. While host cells elaborate diverse mechanisms for pathogen expulsion, amebae have also developed complex strategies to modulate host immune response and facilitate their own survival. This paper will give an overview of current research on the mutual interactions between host and Entamoeba histolytica in human and experimental amebiasis. Understanding this crosstalk is crucial for the effective design and implementation of new vaccines and drugs for this leading parasitic disease.

show abstract

“…The mechanisms of human resistance to colonization or invasive disease are poorly understood. One contributing factor is fecal IgA to the parasite, particularly to its galactose-inhibitable adherence lectin, which correlates with partial protection against infection in children and vaccinated mice (3,4). Additionally, a protective role for IFN-␥, TNF-␣, and NO on macrophages has been demonstrated in vitro (5).…”

Section: Resistance Of C57bl/6 Mice To Amoebiasis Is Mediated By Nonhmentioning

confidence: 99%

“…To define the host mechanisms of resistance to intestinal amoebiasis, we have used a mouse model of amoebic colitis that develops upon intracecal inoculation of trophozoites (4,8,9). A majority of CBA or C3H (either TLR4 mutant or wild-type (WT)) 3 mice develop patent infections upon challenge.…”

Section: Resistance Of C57bl/6 Mice To Amoebiasis Is Mediated By Nonhmentioning

confidence: 99%

Resistance of C57BL/6 Mice to Amoebiasis Is Mediated by Nonhemopoietic Cells but Requires Hemopoietic IL-10 Production

Hamano

Asgharpour

Stroup

et al. 2006

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Resistance to intestinal amoebiasis is mouse strain dependent. C57BL/6 (B6) mice clear Entamoeba histolytica within hours of challenge, whereas C3H and CBA strains are susceptible to infection and disease. In this study, we show using bone marrow (BM) chimeric mice that mouse strain-dependent resistance is mediated by nonhemopoietic cells; specifically, B6 BM → CBA recipients remained susceptible as measured by amoeba score and culture, whereas CBA BM → B6 recipients remained resistant. Interestingly, hemopoietic IL-10 was required for maintaining the resistance of B6 mice, in that B6 IL-10-deficient mice and IL-10−/− BM → wild-type recipients, but not IL-10+/+ BM → IL-10−/− recipients, exhibited higher amoeba scores than their wild-type controls. Additionally, C57BL/10 IL-10−/−Rag2−/− mice exhibited diminished amoeba scores and culture rates vs IL-10−/− mice, indicating that lymphocytes potentiated the susceptibility of IL-10-deficient mice. We conclude that nonhemopoietic cells mediate the natural resistance to intestinal amoebiasis of B6 mice, yet this resistance depends on hemopoietic IL-10 activity.

show abstract

Prevention of intestinal amebiasis by vaccination with the Entamoeba histolytica Gal/GalNac lectin

Cited by 94 publications

References 29 publications

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies

Resistance of C57BL/6 Mice to Amoebiasis Is Mediated by Nonhemopoietic Cells but Requires Hemopoietic IL-10 Production

Contact Info

Product

Resources

About