Prevention of liver fibrosis and liver reconstitution of DMN‐treated rat liver by transplanted EPCs

Nakamura, Tõru; Torimura, Takuji; Iwamoto, Hideki; Masuda, Hiroshi; Naitou, Masako; Koga, Hironori; Abe, Mitsuhiko; Hashimoto, Osamu; Tsutsumi, Vı́ctor; Ueno, Takato; Sata, Michio

doi:10.1111/j.1365-2362.2011.02637.x

Cited by 26 publications

(24 citation statements)

References 43 publications

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“…In addition, IHC of frozen sections confirmed that more blood vessels were formed in the liver after treatment with HD cultured cells [15]. The enhancement of capillary density was coinciding with the treatment by EPCs Prevention of liver fibrosis and liver reconstitution of DMN-treated rat liver by transplanted EPCs [28]. Interestingly, these findings are conflicting with the reports that liver fibrogenesis and angiogenesis develop in parallel during progression towards cirrhosis [29], [30].…”

Section: Discussionmentioning

confidence: 90%

Prevention of Liver Fibrosis by Intrasplenic Injection of High-Density Cultured Bone Marrow Cells in a Rat Chronic Liver Injury Model

Lian

Yang

et al. 2014

PLoS ONE

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Endothelial progenitor cells (EPCs) from bone marrow have proven to be functional for the prevention of liver fibrosis in chronic liver injury. However, expansion of EPCs in culture is complicated and expansive. Previously, we have established a simple method that could enrich and expand EPCs by simple seeding bone marrow cells in high density dots. The purpose of this study is to evaluate whether cells derived from high-density (HD) culture of rat bone marrow cells could prevent the liver fibrosis in a chronic liver injury rat model, induced by carbon tetrachloride (CCl4). Flow cytometric analysis showed that cells from HD culture were enriched for EPCs, expressing high levels of EPC markers. Intrasplenic injection of HD cultured bone marrow cells in the CCl4-induced liver injury rat showed an enhanced antifibrogenic effect compared with animals treated with cells from regular-density culture. The antifibrogenic effect was demonstrated by biochemical and histological analysis 4 weeks post-transplantation. Furthermore, cells from HD culture likely worked through increasing neovascularization, stimulating liver cell proliferation, and suppressing pro-fibrogenic factor expression. HD culture, which is a simple and cost-effective procedure, could potentially be used to expand bone marrow cells for the treatment of liver fibrosis.

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Section: Discussionmentioning

confidence: 90%

Prevention of Liver Fibrosis by Intrasplenic Injection of High-Density Cultured Bone Marrow Cells in a Rat Chronic Liver Injury Model

Lian

Yang

et al. 2014

PLoS ONE

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“…VEGF, SDF‐1 and IGF‐1) that promote the migration of resident progenitor cells and mature endothelial cells to contribute to neovascularization . The important role of EPCs is supported by the finding that their levels are reduced or dysfunctional in some diseases such as diabetes , and also by the functional improvement of tissues after EPC transplantation in different disease models .…”

Section: Adult Stem Cell Migration In Physiological and Pathological mentioning

confidence: 99%

Importance and regulation of adult stem cell migration

Lucas

Pérez

Gálvez

2017

J Cellular Molecular Medi

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Cell migration is an essential process throughout the life of vertebrates, beginning during embryonic development and continuing throughout adulthood. Stem cells have an inherent ability to migrate, that is as important as their capacity for self-renewal and differentiation, enabling them to maintain tissue homoeostasis and mediate repair and regeneration. Adult stem cells reside in specific tissue niches, where they remain in a quiescent state until called upon and activated by tissue environmental signals. Cell migration is a highly regulated process that involves the integration of intrinsic signals from the niche and extrinsic factors. Studies using three-dimensional in vitro models have revealed the astonishing plasticity of cells in terms of the migration modes employed in response to changes in the microenvironment. These same properties can, however, be subverted during the development of some pathologies such as cancer. In this review, we describe the response of adult stem cells to migratory stimuli and the mechanisms by which they sense and transduce intracellular signals involved in migratory processes. Understanding the molecular events underlying migration may help develop therapeutic strategies for regenerative medicine and to treat diseases with a cell migration component.

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“…The critical role of EPCs is suggested by their dysfunction and reduced levels in clinical disease states associated with poor wound healing, such as diabetes [47,48], and the observation that EPC transplantation can ameliorate injury and improve functional outcomes in models of stroke [13], myocardial infarction [14] and acute liver and lung injury [15,16]. …”

Section: Endothelial Progenitor Cellsmentioning

confidence: 99%

Stem Cell Recruitment After Injury: Lessons for Regenerative Medicine

et al. 2012

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Tissue repair and regeneration are thought to involve resident cell proliferation as well as the selective recruitment of circulating stem and progenitor cell populations through complex signaling cascades. Many of these recruited cells originate from the bone marrow, and specific subpopulations of bone marrow cells have been isolated and used to augment adult tissue regeneration in preclinical models. Clinical studies of cell-based therapies have reported mixed results, however, and a variety of approaches to enhance the regenerative capacity of stem cell therapies are being developed based on emerging insights into the mechanisms of progenitor cell biology and recruitment following injury. This article discusses the function and mechanisms of recruitment of important bone marrow-derived stem and progenitor cell populations following injury, as well as the emerging therapeutic applications targeting these cells.

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Prevention of liver fibrosis and liver reconstitution of DMN‐treated rat liver by transplanted EPCs

Abstract: EPC transplantation effectively promotes the remodelling of tissues damaged by liver fibrosis; it can also reconstitute sinusoids in chronic liver injury.

Cited by 26 publications

References 43 publications

Prevention of Liver Fibrosis by Intrasplenic Injection of High-Density Cultured Bone Marrow Cells in a Rat Chronic Liver Injury Model

Prevention of Liver Fibrosis by Intrasplenic Injection of High-Density Cultured Bone Marrow Cells in a Rat Chronic Liver Injury Model

Importance and regulation of adult stem cell migration

Stem Cell Recruitment After Injury: Lessons for Regenerative Medicine

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