Prevention of Peritoneal Adhesions by Ferric Ion-Cross-Linked Hydrogels of Hyaluronic Acid Modified with Iminodiacetic Acids

Amano, Yuki; Pan, Qi; Nakagawa, Yoshiyuki; Kirita, Katsuhisa; Ohta, Seiichi; Ito, Taichi

doi:10.1021/acsbiomaterials.8b00456

Cited by 18 publications

(10 citation statements)

References 37 publications

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“…The efficacy of the spray delivery method and materials on tissue adhesion prevention were evaluated using the rat hepatectomy-induced adhesion model (Figure ). The cecal abrasion model and uterine horn model are standard tissue adhesion models for animal studies and have been widely used. − However, we used a more intense rat hepatectomy-induced adhesion model we developed recently . In the field of liver surgery, repeat hepatectomy is a highly effective treatment for intrahepatic recurrence, but the dissected surface after hepatectomy forms a severe adhesion over a wide range with the digestive organ and diaphragm .…”

Section: Discussionmentioning

confidence: 99%

In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion

Pan

Zheng

Ohta

et al. 2019

ACS Biomater. Sci. Eng.

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Postoperative peritoneal adhesions could cause pelvic pain, infertility, and bowel obstruction. In addition, adhesiolysis makes second surgery difficult. For the first time, we fabricated double-layered hydrogels in situ on the trauma surface via sequential double spray processes to prevent peritoneal adhesions. The spray conditions were optimized for spray distance and gas flow rate to create homogeneous and seamless double-layered hydrogels. The top layer was composed of alginate (Alg)−carboxymethyl cellulose (CMC) and serves as the barrier between the wounded tissue and surrounding tissues. The bottom layer was composed of Alg−gelatin (Gela) and comes in direct contact with the wounded tissue to promote wound healing. In vitro experiments showed that the Alg− Gela hydrogel layer promoted wound healing by accelerating horizontal cell migration. In addition, the Alg−CMC layer prevented the vertical penetration of fibroblast cells. The prevention efficacy of the sprayable double-layered hydrogels was evaluated using a partial hepatectomy-induced adhesion model in rats. The double-layered hydrogels decreased the adhesion grade and extent of liver cut surface, whereas the two single-layered hydrogels, Alg−CMC and Alg−Gela, did not show any adhesion prevention efficacy.

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Section: Discussionmentioning

confidence: 99%

In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion

Pan

Zheng

Ohta

et al. 2019

ACS Biomater. Sci. Eng.

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Section: Biomaterials To Fabricate Antiadhesion Barriersmentioning

confidence: 99%

Advancement of Biomaterial‐Based Postoperative Adhesion Barriers

Chandel

Shimizu

Hasegawa

et al. 2021

Macromolecular Bioscience

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Postoperative peritoneal adhesion (PPA) is a prevalent incidence that generally happens during the healing process of traumatized tissues. It causes multiple severe complications such as intestinal obstruction, chronic abdominal pain, and female infertility. To prevent PPA, several antiadhesion materials and drug delivery systems composed of biomaterials are used clinically, and clinical antiadhesive is one of the important applications nowadays. In addition to several commercially available materials, like film, spray, injectable hydrogel, powder, or solution type have been energetically studied based on natural and synthetic biomaterials such as alginate, hyaluronan, cellulose, starch, chondroitin sulfate, polyethylene glycol, polylactic acid, etc. Moreover, many kinds of animal adhesion models, such as cecum abrasion models and unitary horn models, are developed to evaluate new materials’ efficacy. A new animal adhesion model based on hepatectomy and conventional animal adhesion models is recently developed and a new adhesion barrier by this new model is also developed. In summary, many kinds of materials and animal models are studied; thus, it is quite important to overview this field's current progress. Here, PPA is reviewed in terms of the species of biomaterials and animal models and several problems to be solved to develop better antiadhesion materials in the future are discussed.

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“…The entangled molecular networks of HyA still retain flowability before crosslinking . The crosslinking of HyA can be achieved via interactions between its carboxyl groups and cations such as Fe 3+ ions, , blending with other polymers, , or modification with other functional groups. − These three types of methods for crosslinking HyA have been explored for a variety of biomedical applications, such as tissue repair, − drug delivery, , cell encapsulation, , and biosensing. , For example, HyA hydrogel crosslinked via carboxylate-Fe 3+ coordination has been clinically used as an adhesion barrier gel, commercially called Intergel, for preventing peritoneal adhesion after surgeries such as laparotomy. ,, Blending HyA with other polymers could also produce crosslinked hydrogels. For example, when the oxidized HyA (aldehyde HyA) was blended with N -succinyl-chitosan, the gelation was achieved via the Schiff base reaction between the aldehyde groups on the oxidized HyA and amino groups from the N -succinyl-chitosan .…”

Section: Introductionmentioning

confidence: 99%

“…Last, HyA can be chemically modified to form HyA derivatives by adding new functional groups, and these HyA derivatives can be crosslinked with or without adding a crosslinking initiator. For example, the iminodiacetic acid (IDA)-modified HyA relied on the addition of cations to achieve the crosslinking via metal–ligand coordination . The methacrylate-modified HyA (MeHyA) was crosslinked under ultraviolet (UV) irradiation with the addition of a photoinitiator .…”

Section: Introductionmentioning

confidence: 99%

Tunable Crosslinking, Reversible Phase Transition, and 3D Printing of Hyaluronic Acid Hydrogels via Dynamic Coordination of Innate Carboxyl Groups and Metallic Ions

Hung

Cao

et al. 2021

ACS Appl. Bio Mater.

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This article reports tunable crosslinking, reversible phase transition, and three-dimensional printing (3DP) of hyaluronic acid (HyA) hydrogels via dynamic coordination of Fe 3+ ions with their innate carboxyl groups for the first time. The concentrations of Fe 3+ and H + ions and the reaction time determine the tunable ratios of mono-, bi-, and tridentate coordination, leading to the low-to-high crosslinking densities and reversible solid−liquid phase transition of HyA hydrogels. At the monodentate-dominant coordination, the liquid hydrogels have low crosslinking densities (HyA_L). At the mixed coordination of mono-, bi-, and tridentate bonding, the solid hydrogels have medium crosslinking densities (HyA_M). At the tridentate-dominant coordination, the solid hydrogels have high crosslinking densities (HyA_H). The reversible solid−liquid phase transitions among HyA_L, HyA_M, and HyA_H were achieved via controlling the concentrations of Fe 3+ and H + ions and reaction time. When the crosslinking densities are between HyA_L and HyA_M, the hydrogels become 3D printable (HyA_P). HyA_P hydrogels were 3D-printed successfully using cold-stage or direct writing methods, and the 3D constructs achieved better structural stability using the latter method. In the direct exposure culture with bone marrow-derived mesenchymal stem cells, the 3D-printed HyA_H (HyA_H_3D) and HyA_H hydrogels showed higher average cell adhesion densities than the HyA_M, HyA_P, and HyA_L hydrogel groups under both direct and indirect contact conditions. For all hydrogel groups, cell adhesion densities under direct contact conditions were statistically lower than the same groups under indirect contact conditions. In this article, we elucidated the mechanisms of dynamic coordination and the relationships among the key parameters in controlling the tunable crosslinking, reversible phase transition, and 3DP of HyA hydrogels without blending with other polymers or adding functional groups. This approach can be potentially adapted to crosslink and 3D print other polymeric hydrogels with carboxyl groups, which is promising for a wide range of applications.

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Prevention of Peritoneal Adhesions by Ferric Ion-Cross-Linked Hydrogels of Hyaluronic Acid Modified with Iminodiacetic Acids

Cited by 18 publications

References 37 publications

In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion

In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion

Advancement of Biomaterial‐Based Postoperative Adhesion Barriers

Tunable Crosslinking, Reversible Phase Transition, and 3D Printing of Hyaluronic Acid Hydrogels via Dynamic Coordination of Innate Carboxyl Groups and Metallic Ions

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