“…The VF, TF, ZF, and EF groups were superficially treated with 1.23% acidulated phosphate fluoride (DFL, Rio de Janeiro, RJ, Brazil), pH 5, which was composed of sodium fluoride, cellulose, fluoridric acid, phosphoric acid, propylenoglicol, colorant, flavor, and deionized water. APF (1.23%) was applied for 4 min (14,15) immediately after the specimen preparation and again after a 24-hour period to simulate fluoride application twice a year (16). The fluoride excess was removed by a suction canula, and the specimens were kept in 5 mL of artificial saliva, pH 7 (Fig.…”