Preventive and synbiotic effects of the soluble dietary fiber obtained from <i>Lentinula edodes</i> byproducts and <i>Lactobacillus plantarum</i><scp>LP90</scp> against dextran sulfate sodium‐induced colitis in mice

Xue, Zihan; Li, Ruilin; Liu, Junyu; Zhou, Jie; Zhang, Xiaoyu; Zhang, Tingting; Zhang, Min; Yang, Yang; Chen, Hạixia

doi:10.1002/jsfa.12173

Cited by 11 publications

(4 citation statements)

References 51 publications

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“…Studies in mice models have shown the enhanced e cacy of synbiotics in alleviating gut in ammation and restoration of the microbiome. [23][24][25] Studies conducted on colorectal cancer models showed the ability of synbiotics to prevent the formation of pre-neoplastic lesions indicating its potential in reducing the risk of colon cancer.…”

Section: Discussionmentioning

confidence: 99%

Beneficial effect of synbiotic combination of Limosilactobacillus fermentum FS-10, Lactiplantibacillus plantarum Lp1-IC and short-chain Fructooligosaccharides in colitis murine model

Pujari,

Jadhav,

Angal

et al. 2024

Preprint

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Therapies targeting gut-microbiota are being extensively researched for colitis patients. In this study, we have tested the efficacy of indigenously isolated strains Lactiplantibacillus plantarum Lp1-IC and Limosilactobacillus fermentum FS-10 and their combination with short chain fructooligosaccharides (sc-FOS) in mice models of DSS-induced colitis. For a desired efficacy, a synbiotic should be very meticulously formulated with the right choice of prebiotic and probiotic. Therefore, the ability of Lactobacilli to selectively utilize scFOS for growth was first tested by culturing the strains in a specially designed minimal media supplemented with scFOS as carbon source. The bacteria utilized scFOS and produced metabolites such as acetate and lactate. Thereafter, the in vitro anti-inflammatory effect was tested on markers such as TNF-alpha (TNF-α), Nitric Oxide and IL-10 in human monocyte (THP-1) and mouse macrophage (Raw 264-7) cell lines. The in vivo efficacy was studied in mice model of DSS-induced colitis and the effect on the systemic and localized inflammatory markers were assessed in serum and colon tissues samples respectively. Administration of DSS elicited predominant clinical signs of weight loss, diarrhoea, fecal occult blood, increase in inflammatory markers and extensive damage of colon tissue. These symptoms were significantly reversed in all the treatment groups, however the combination of lactobacilli and scFOS performed better than the individual ingredients. The study highlights the potential of the indigenous lactobacilli strains, scFOS and their combination for management of gut-inflammation in colitis patients.

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Section: Discussionmentioning

confidence: 99%

Beneficial effect of synbiotic combination of Limosilactobacillus fermentum FS-10, Lactiplantibacillus plantarum Lp1-IC and short-chain Fructooligosaccharides in colitis murine model

Pujari,

Jadhav,

Angal

et al. 2024

Preprint

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“…In a recent study, we discovered that soluble dietary fiber from Lentinula edodes byproducts combined with Lactobacillus plantarum LP90 repaired intestinal epithelial injury in dextran sulfate sodium-induced colitis mice. Additionally, the synbiotic therapy enhanced the production of butyric acid and upregulated the expression of tight junction proteins [ 101 ].…”

Section: Sources Of Polysaccharides and Their Anti-diabetic Effectsmentioning

confidence: 99%

Updated Progress on Polysaccharides with Anti-Diabetic Effects through the Regulation of Gut Microbiota: Sources, Mechanisms, and Structure–Activity Relationships

Zhang,

Wang,

Zhang

et al. 2024

Pharmaceuticals

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Diabetes mellitus (DM) is a common chronic metabolic disease worldwide. The disturbance of the gut microbiota has a complex influence on the development of DM. Polysaccharides are one type of the most important natural components with anti-diabetic effects. Gut microbiota can participate in the fermentation of polysaccharides, and through this, polysaccharides regulate the gut microbiota and improve DM. This review begins by a summary of the sources, anti-diabetic effects and the gut microbiota regulation functions of natural polysaccharides. Then, the mechanisms of polysaccharides in regulating the gut microbiota to exert anti-diabetic effects and the structure–activity relationship are summarized. It is found that polysaccharides from plants, fungi, and marine organisms show great hypoglycemic activities and the gut microbiota regulation functions. The mechanisms mainly include repairing the gut burrier, reshaping gut microbiota composition, changing the metabolites, regulating anti-inflammatory activity and immune function, and regulating the signal pathways. Structural characteristics of polysaccharides, such as monosaccharide composition, molecular weight, and type of glycosidic linkage, show great influence on the anti-diabetic activity of polysaccharides. This review provides a reference for the exploration and development of the anti-diabetic effects of polysaccharides.

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“…The identi cation of bacteria exhibiting differential abundance was conducted using various statistical methods, including ANCOM, ANOVA, Kruskal Wallis, LEfSe, and DEseq2 [26,27]. The R language ggtree package (v1.4.11) was used to visualize the evolutionary tree.…”

Section: Experimental Data Processingmentioning

confidence: 99%

Assessing the Impact of Triiodothyronine Treatment on the Lung Microbiome of Mice with Pulmonary Fibrosis

Guo,

Xu,

Wang

et al. 2023

Preprint

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Background Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease characterized by progressive pulmonary fibrosis (PF) exacerbation. IPF primarily affects older individuals and can lead to respiratory failure. This study aimed to assess the effects of triiodothyronine (T3) treatment on the lung microbiome of mice with PF. Methods Mice were perfused with bleomycin (BLM) to establish a PF model. Using a randomized design, 40 female specific pathogen-free (SPF) C57BL6/N mice were divided into four groups: saline, saline + T3, BLM, and BLM + T3. Hematoxylin and eosin and Masson’s trichrome staining were used to detect histological morphology, and 16S rRNA microbial sequencing was used to identify lung bacteria. An Enzyme-Linked Immunosorbent Assay was used to measure total T3(TT3), free T3(FT3, and reverse T3(rT3) levels in the peripheral serum. Results T3treatment ameliorated BLM-induced lung fibrosis and structural damage. The microbiome experienced a decrease in the abundance of Proteobacteria, Bacteroides, and Actinomycetes and an increase in the abundance of Firmicutes when exposed to BLM; however, T3treatment reversed this effect. The four groups showed no significant difference in alpha and beta microbiome diversity (P > 0.05), and the effect of T3treatment and BLM treatment on lung microbial alpha diversity was in the opposite direction, which was of positive significance; beta-diversity analysis suggested a relatively stable microbiome). Serum concentrations of TT3and FT3were positively correlated with microbiome abundance (P < 0.05). Administration of T3enhanced the microbiota in PF without affecting the diversity and biological functions of the microbiome (P > 0.05). Conclusion T3treatment had a positive effect on the lung microbiome of mice with PF. This finding partially supports the use of T3as a potential drug for the treatment of PF.

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Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarumLP90 against dextran sulfate sodium‐induced colitis in mice

Cited by 11 publications

References 51 publications

Beneficial effect of synbiotic combination of Limosilactobacillus fermentum FS-10, Lactiplantibacillus plantarum Lp1-IC and short-chain Fructooligosaccharides in colitis murine model

Beneficial effect of synbiotic combination of Limosilactobacillus fermentum FS-10, Lactiplantibacillus plantarum Lp1-IC and short-chain Fructooligosaccharides in colitis murine model

Updated Progress on Polysaccharides with Anti-Diabetic Effects through the Regulation of Gut Microbiota: Sources, Mechanisms, and Structure–Activity Relationships

Assessing the Impact of Triiodothyronine Treatment on the Lung Microbiome of Mice with Pulmonary Fibrosis

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