Preventive and Therapeutic Effects of Lactobacillus Paracasei B21060–Based Synbiotic Treatment on Gut Inflammation and Barrier Integrity in Colitic Mice

Simeoli, Raffaele; Raso, Giuseppina Mattace; Lama, Adriano; Pirozzi, Claudio; Santoro, Anna; Guida, Francesca; Sanges, M.; Aksoy, Ezra; Calignano, Antonio; D’Arienzo, A.; Meli, Rosaria

doi:10.3945/jn.114.205989

Cited by 46 publications

(26 citation statements)

References 42 publications

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“…L. acidophilus blocks the colitis-associated immune response of the IL-23/Th17 axis by downregulating the activity of IL-17, TNFα, IL-23, TGFβ1, and STAT3 36 . L. paracasei has been shown to induce antiinflammatory responses in IBD, including chemically and pathogenically induced colitis models [37][38][39] . Moreover, Fig.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus paracasei-derived extracellular vesicles attenuate the intestinal inflammatory response by augmenting the endoplasmic reticulum stress pathway

Choi

Moon

Shin³

et al. 2020

Exp Mol Med

124

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Lactobacillus paracasei is a major probiotic and is well known for its anti-inflammatory properties. Thus, we investigated the effects of L. paracasei-derived extracellular vesicles (LpEVs) on LPS-induced inflammation in HT29 human colorectal cancer cells and dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice. ER stress inhibitors (salubrinal or 4-PBA) or CHOP siRNA were utilized to investigate the relationship between LpEV-induced endoplasmic reticulum (ER) stress and the inhibitory effect of LpEVs against LPS-induced inflammation. DSS (2%) was administered to male C57BL/6 mice to induce inflammatory bowel disease, and disease activity was measured by determining colon length, disease activity index, and survival ratio. In in vitro experiments, LpEVs reduced the expression of the LPSinduced pro-inflammatory cytokines IL-1α, IL-1β, IL-2, and TNFα and increased the expression of the anti-inflammatory cytokines IL-10 and TGFβ. LpEVs reduced LPS-induced inflammation in HT29 cells and decreased the activation of inflammation-associated proteins, such as COX-2, iNOS and NFκB, as well as nitric oxide. In in vivo mouse experiments, the oral administration of LpEVs also protected against DSS-induced colitis by reducing weight loss, maintaining colon length, and decreasing the disease activity index (DAI). In addition, LpEVs induced the expression of endoplasmic reticulum (ER) stress-associated proteins, while the inhibition of these proteins blocked the anti-inflammatory effects of LpEVs in LPS-treated HT29 cells, restoring the pro-inflammatory effects of LPS. This study found that LpEVs attenuate LPS-induced inflammation in the intestine through ER stress activation. Our results suggest that LpEVs have a significant effect in maintaining colorectal homeostasis in inflammation-mediated pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus paracasei-derived extracellular vesicles attenuate the intestinal inflammatory response by augmenting the endoplasmic reticulum stress pathway

Choi

Moon

Shin³

et al. 2020

Exp Mol Med

124

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“…Colon tissue was homogenized, and protein lysates were subjected to SDS‐PAGE as described previously (Simeoli et al ., ). The filters were probed with primary antibody overnight.…”

Section: Methodsmentioning

confidence: 97%

An orally administered butyrate‐releasing derivative reduces neutrophil recruitment and inflammation in dextran sulphate sodium‐induced murine colitis

Simeoli

Raso

Pirozzi

et al. 2016

British J Pharmacology

Self Cite

101

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“…Various strains of probiotics could control microbiota, reduce colitis symptoms, protect barrier integrity and inhibit the release of proinflammatory cytokines [47] , resulting in preventing or repairing gut damage and inflammatory responses induced by pathogens [48] . However, the mechanisms behind these probiotics are complicated.…”

Section: Probiotics In Gi Health and Digestive Diseasesmentioning

confidence: 99%

Vitamin D/VDR, Probiotics, and Gastrointestinal Diseases

Shang

Sun

2017

CMC

105

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Vitamin D is an important factor in regulating inflammation, immune responses, and carcinoma inhibition via action of its receptor, vitamin D receptor (VDR). Recent studies have demonstrated the role of vitamin D/VDR in regulating host-bacterial interactions. Probiotics are beneficial bacteria with the power of supporting or favoring life on the host. In the current review, we will discuss the recent progress on the roles of vitamin D/VDR in gut microbiome and inflammation. We will summarize evidence of probiotics in modulating vitamin D/VDR and balancing gut microbiota in health and gastrointestinal diseases. Moreover, we will review the clinical application of probiotics in prevention and therapy of IBD or colon cancer. Despite of the gains, there remain several barriers to advocate broad use of probiotics in clinical therapy. We will also discuss the limits and future direction in scientific understanding of probiotics, vitamin D/VDR, and host responses.

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Preventive and Therapeutic Effects of Lactobacillus Paracasei B21060–Based Synbiotic Treatment on Gut Inflammation and Barrier Integrity in Colitic Mice

Abstract: Our data highlight the beneficial effects of this synbiotic formulation in acutely colitic mice, suggesting that it may have therapeutic and possibly preventive efficacy in human colitis.

Cited by 46 publications

References 42 publications

Lactobacillus paracasei-derived extracellular vesicles attenuate the intestinal inflammatory response by augmenting the endoplasmic reticulum stress pathway

Lactobacillus paracasei-derived extracellular vesicles attenuate the intestinal inflammatory response by augmenting the endoplasmic reticulum stress pathway

An orally administered butyrate‐releasing derivative reduces neutrophil recruitment and inflammation in dextran sulphate sodium‐induced murine colitis

Vitamin D/VDR, Probiotics, and Gastrointestinal Diseases

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