First aid training is necessary for engineering students to increase their health and safety knowledge relating to reducing damage and suffering and improving their chances of survival. Objective: This study aims to evaluate the effectiveness of a training program by blended learning on knowledge and practices regarding first aid among preparatory year engineering students at Assiut University. A pretest, posttest design was used in this study. About 85 participants were selected randomly from the preparatory year of engineering students at Assiut University. Participants were randomly selected and provided with a first-aid training program. Data were collected via a self-administered knowledge questionnaire sheet and an observational checklist pre, post, and three months after the program intervention. The repeated measured ANOVA was used. Results: The study revealed that 61.2% of students had poor knowledge scores about first aid in the pre-test with a total mean of 65.16±24.41, which improved to 95.3% of students who had good knowledge scores in the post-test with a total mean of 121.89±10.27, and slightly declined to 77.6% having good knowledge scores in the follow-up test with a total mean of 110.93±27.38. Moreover, 85.9% of students had poor practice scores about first aid in the pre-test with a total mean of 19.19±4.71, which improved to 72.9% having good practice scores in the post-test with a total mean of 52.49±7.84, and 65.9% having good practices scores in the follow-up test with a total mean of 48.64±13.49. The study concluded that the training program by blended learning showed a positive effect on knowledge and practices of students at post and follow-up tests. This provides a safe learning environment for engineering students. Recommendation: Blended learning programs should be taken as a learning approach in the health training program implementation.