Preventive Effect of Clostridium butyricum M588 against the Proliferation of Clostridium difficile during Antimicrobial Therapy

Kuroiwa, Toyoaki; Iwanaga, Masaaki; Kobari, Kazumine; Higashionna, Atsushi; Kinjyo, Fukunori; Saito, Atsushi

doi:10.11150/kansenshogakuzasshi1970.64.1425

Cited by 12 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of the KEGG categories revealed that vitamin, amino acid and O-glycan biosynthesis were the most enriched metabolic pathways. C. butyricum ferments in the cecum and thus provides a large number of SCFA, amino acids, vitamins and other metabolites to the host ( Kuroiwa et al., 1990 ; Yang et al., 2012 ). These abundant nutrients are transported into epithelial cells of the cecal mucosa through the upregulation of carrier genes, such as solute carrier family 7 member 11 ( SLC7A11 ), low-density lipoprotein-related protein 2 ( LRP2 ), translocator protein 2 ( TSPO2 ), hemoglobin subunit mu ( HBM ), hemoglobin subunit alpha 1 ( HBA1 ) and ENSGALG00000050921 (very-long-chain fatty acid-CoA ligase activity), which might shed light on the mechanisms through which dietary supplementation with C. butyricum can improve growth performance ( Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells

Sun

Wan

et al. 2021

Animal Nutrition

View full text Add to dashboard Cite

Clostridium butyricum has been widely considered an antibiotic substitute in recent years. It can promote growth performance, improve the immune response and enhance the intestinal barrier function of the host. In the present study, 1-d-old Arbor Acres (AA) broilers were fed C. butyricum (1 × 10 9 cfu/kg) for 28 d. The transcriptomic characteristics of epithelial cells of the cecal mucosa were determined by RNA-sequence, and the cecal microbiota composition was explored by 16S ribosomal RNA gene sequencing. The changes in the intestinal mucosa of broilers were then analyzed by tissue staining. Gene Ontology (GO) annotations identified substance transport and processes and pathways that might participate in intestinal development and cell viability. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the differentially expressed genes are involved in numerous pathways related to amino acid and vitamin metabolism and antioxidant and defensive functions, among others. The relative expression of some genes associated with intestinal barrier function (claudins 2, 15, 19, and 23, tight junction proteins 1, 2, and 3 and mucin 1) was significantly increased in the treatment group ( P < 0.05 or P < 0.01). Moreover, the proportion of Firmicutes was higher in the C. butyricum -treated group, whereas the proportion of Proteobacteria was higher in the control group. At the genus level, the relative abundances of Butyricicoccus and Lactobacillus , among other bacteria, were increased after C. butyricum supplementation. The tissue staining analysis showed that the cecal mucosa of broilers was significantly ameliorated after the addition of C. butyricum ( P < 0.05 or P < 0.01). These results showed that dietary supplementation with C. butyricum can enhance the antioxidant capacity, mucosal barrier function, and stabilize the cecal microbiota, resulting in improving the growth performance.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells

Sun

Wan

et al. 2021

Animal Nutrition

View full text Add to dashboard Cite

show abstract

“…C. difficile is a common pathogen responsible for a large proportion of AAD cases, and in a human study C. butyricum administration was associated with decreased fecal levels of C. difficile as well as the toxin it produces. 79 More recently, Woo and colleagues demonstrated that in vitro co-culture of C. difficile with CBM 588 decreased the pathogen’s toxicity in a dose-dependent manner. 80 Oka et al .…”

Section: Evidence From Murine Models Of Diseasementioning

confidence: 99%

Butyrate-producing human gut symbiont, Clostridium butyricum, and its role in health and disease

Stoeva¹,

Garcia-So²,

Justice³

et al. 2021

Gut Microbes

234

156

View full text Add to dashboard Cite

Clostridium butyricum is a butyrate-producing human gut symbiont that has been safely used as a probiotic for decades. C. butyricum strains have been investigated for potential protective or ameliorative effects in a wide range of human diseases, including gut-acquired infection, intestinal injury, irritable bowel syndrome, inflammatory bowel disease, neurodegenerative disease, metabolic disease, and colorectal cancer. In this review we summarize the studies on C. butyricum supplementation with special attention to proposed mechanisms for the associated health benefits and the supporting experimental evidence. These mechanisms center on molecular signals (especially butyrate) as well as immunological signals in the digestive system that cascade well beyond the gut to the liver, adipose tissue, brain, and more. The safety of probiotic C. butyricum strains appears well-established. We identify areas where additional human randomized controlled trials would provide valuable further data related to the strains' utility as an intervention.

show abstract

“…Shimbo et al (2005) and Imase et al (2008) observed stabilising effects on the intestinal microbiota when CBM 588 was administered as a supplement during the antibiotic eradication of Helicobacter pylori infection. Furthermore, it has been found that CBM 588 is able to suppress the production of Clostridium difficile toxins after antimicrobial therapy in humans and also to inhibit some strains of Vibrio cholerae, Aeromonas hydrophila, and Shigella flexneri in mixed cultures (Kuroiwa et al 1990). CBM 588 was also tested in rats, wherein it was able to mitigate the symptoms of dextran sulphate sodium-induced colitis and also increased the counts of lactobacilli in their gut (Okamoto et al 2000).…”

mentioning

confidence: 99%

Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens

Švejstil¹,

Plachý²,

Joch³

et al. 2019

CZECH J ANIM SCI

View full text Add to dashboard Cite

Clostridium butyricum CBM 588 is used as a probiotic in eastern Asian countries and has been recently approved as an animal feed additive in the European Union. The purpose of this study was to evaluate the effect of C. butyricum MIYAIRI 588 (CBM 588) on abundance of selected genera of caecal and crop bacteria, volatile fatty acids and growth performance of broiler chickens. We studied counts of anaerobic bacteria in caeca and crops of broiler chickens by plate-count method and evaluated their growth performance. CBM 588 significantly reduced E. coli counts in caeca of broiler chickens at days 10 and 42 and also enhanced their growth performance. Additionally, it significantly increased the amount of butyrate in the caeca that provides energy to enterocytes, resulting in increased weight gains. Out of the obtained results we conclude that C. butyricum CBM 588 influences caecal microbiota of broiler chickens and positively affects their growth performance.

show abstract

Preventive Effect of Clostridium butyricum M588 against the Proliferation of Clostridium difficile during Antimicrobial Therapy

Cited by 12 publications

References 0 publications

Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells

Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells

Butyrate-producing human gut symbiont, Clostridium butyricum, and its role in health and disease

Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens

Contact Info

Product

Resources

About