Preventive Effect of Combined Zingiber officinale and Terminalia chebula against DMBA-Induced Breast Cancer Rats via mTOR Inhibition

Mathiyazhagan, Jayasindu; Siva, Ramamoorthy; Jayaraj, Rama; Madhyastha, Harishkumar; Gothandam, Kodiveri Muthukaliannan

doi:10.1080/01635581.2021.1903948

Cited by 7 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The immunohistochemistry analysis was performed to assess the expression of the various proteins following DMBA-induced breast cancer [46,47]. The slides were visualized using light microscope attached with the camera at 40Â for the analysis of protein expression [46,47].…”

Section: The Immunohistochemistry Analysismentioning

confidence: 99%

N‐(benzylidene)‐2‐((2‐hydroxynaphthalen‐1‐yl)diazenyl)benzohydrazides (1‐2) (NCHDH and NTHDH) attenuate DMBA‐induced breast cancer via Nrf2/NF‐κB/apoptosis signaling

Khan

Shal

et al. 2022

Fundamemntal Clinical Pharma

View full text Add to dashboard Cite

The present study investigated the effect of the N-(benzylidene)-2-((2-hydroxynaphthalen-1-yl)diazenyl)benzohydrazides (1-2) (NCHDH and NTHDH) against breast cancer using in vitro and in vivo approaches. The NCHDH and NTHDH significantly inhibited the growth of the MCF-7 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The NCHDH and NTHDH treatment significantly inhibited the tumor size, tumor weight, and tumor volume, while it enhanced the survival and tumor free survival rate following 7,12-Dimethylbenz[a]anthracene (DMBA)induced breast cancer. The NCHDH and NTHDH markedly attenuated the oxidative stress markers and induced the antioxidant level. The enzymelinked immunosorbent assay (ELISA) showed significant reduction in the inflammatory cytokines production compared with the DMBA control. The NCHDH and NTHDH treatment significantly improved the histological features using hematoxylin and eosin (H and E) staining, Masson's trichrome, PAS (periodic acid Schiff), and Toluidine blue staining compared with the DMBA-induced group. The NCHDH and NTHDH treatment improved the hematological and serological parameters following DMBA-induced breast tumor compared with DMBA-induced group. Furthermore, the NCHDH and NTHDH treatment significantly enhanced the antioxidants signaling proteins such as nuclear factor erythroid 2-related factor 2 (Nrf2) and Heme oxygenase 1 (HO-1). The NCHDH and NTHDH enhanced the inhibitor of NF-κB (IκB) level, while it attenuated the NF-κB level. Similarly, the NCHDH and NTHDH showed marked increase in the apoptosis proteins such as Caspase-3, Caspase-9, and Bcl-2 Associated X-protein (Bax), while it inhibited the B-cell lymphoma 2 (Bcl-2) expression. In conclusion, the NCHDH and NTHDH significantly improved the DMBA-induced breast cancer via attenuating oxidative stress and inflammatory cytokines.

show abstract

Section: The Immunohistochemistry Analysismentioning

confidence: 99%

N‐(benzylidene)‐2‐((2‐hydroxynaphthalen‐1‐yl)diazenyl)benzohydrazides (1‐2) (NCHDH and NTHDH) attenuate DMBA‐induced breast cancer via Nrf2/NF‐κB/apoptosis signaling

Khan

Shal

et al. 2022

Fundamemntal Clinical Pharma

View full text Add to dashboard Cite

show abstract

“…Moreover, it has altered liver metabolic enzymes and induced harmful renal disorders ( Molnar et al, 2022 ) . The adverse initiator and promoter properties of DMBA are attributed to its produced metabolites, which can alkylate DNA or other cellular macromolecules ( Mathiyazhagan et al, 2022 ), trigger the formation of reactive oxygen species (ROS) ( Hosny et al, 2021 ) , alter behavior and cellular dysfunction ( Li et al, 2016 ), and initiate carcinogenicity, particularly in animal research and cancer models ( Zhang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Annona Muricata L. extract restores renal function, oxidative stress, immunohistochemical structure, and gene expression of TNF-α, IL-β1, and CYP2E1 in the kidney of DMBA-intoxicated rats

Zeweil,

Khafaga,

Mahmoud

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Introduction: 7,12-dimethylbenz (a) anthracene (DMBA) is a harmful polycyclic aromatic hydrocarbon derivative known for its cytotoxic, carcinogenic, and mutagenic effects in mammals and other species. Annona muricata, L. (Graviola; GRV) is a tropical fruit tree traditionally well-documented for its various medicinal benefits. This investigation is the first report on the potential antioxidant and antinfammatory reno-protective impact of GRV against DMBA-induced nephrotoxicity in rats.Methods: Forty male albino rats were allocated into four equal groups (n = 10). The 1st group served as the control, the 2nd group (GRV) was gastro-gavaged with GRV (200 mg/kg b.wt), the 3rd group (DMBA) was treated with a single dose of DMBA (15 mg/kg body weight), and the 4th group (DMBA + GRV) was gastro-gavaged with a single dose of DMBA, followed by GRV (200 mg/kg b.wt). The GRV administration was continued for 8 weeks.Results and Discussion: Results revealed a significant improvement in renal function, represented by a decrease in urea, creatinine, and uric acid (UA) in the DMBA + GRV group. The antioxidant potential of GRV was confirmed in the DMBA + GRV group by a significant decline in malondialdehyde (MDA) and a significant increase in catalase (CAT), superoxide dismutase (SOD), glutathione S transferase (GST), and reduced glutathione (GSH) compared to DMBA-intoxicated rats; however, it was not identical to the control. Additionally, the antiinflammatory role of GRV was suggested by a significant decline in mRNA expression of cytochrome P450, family 2, subfamily e, polypeptide 1 (CYP2E1), tumor necrosis factor-alpha (TNF-α), and interleukin 1 beta (IL-1β) in the DMBA + GRV group. Moreover, GRV improved the histopathologic and immunohistochemical expression of TNF-α, CYP450, and IL1β in DMBA-intoxicated kidney tissue. Conclusively, GRV is a natural medicinal product that can alleviate the renal injury resulting from environmental exposure to DMBA. The reno-protective effects of GRV may involve its anti-inflammatory and/or antioxidant properties, which are based on the presence of phytochemical compounds such as acetogenins, alkaloids, and flavonoids.

show abstract

Evaluating the in vitro and in vivo effects of carvacrol zinc oxide quantum dots in breast cancer

Srinivasan,

Namasivayam

2024

Journal of Biomaterials Science, Polymer Edition

View full text Add to dashboard Cite

Preventive Effect of Combined Zingiber officinale and Terminalia chebula against DMBA-Induced Breast Cancer Rats via mTOR Inhibition

Cited by 7 publications

References 35 publications

N‐(benzylidene)‐2‐((2‐hydroxynaphthalen‐1‐yl)diazenyl)benzohydrazides (1‐2) (NCHDH and NTHDH) attenuate DMBA‐induced breast cancer via Nrf2/NF‐κB/apoptosis signaling

N‐(benzylidene)‐2‐((2‐hydroxynaphthalen‐1‐yl)diazenyl)benzohydrazides (1‐2) (NCHDH and NTHDH) attenuate DMBA‐induced breast cancer via Nrf2/NF‐κB/apoptosis signaling

Annona Muricata L. extract restores renal function, oxidative stress, immunohistochemical structure, and gene expression of TNF-α, IL-β1, and CYP2E1 in the kidney of DMBA-intoxicated rats

Evaluating the in vitro and in vivo effects of carvacrol zinc oxide quantum dots in breast cancer

Contact Info

Product

Resources

About