Preventive Effect of<i>Streptococcus thermophilus</i>YIT 2001 on Dextran Sulfate Sodium-Induced Colitis in Mice

Ito, Masahiko; Ohishi, Kenji; Yoshida, Yoshinobu; Okumura, Takeshi; Satô, Tadashi; Yokoi, Wakae; Sawada, Haruji

doi:10.1271/bbb.80240

Cited by 12 publications

(9 citation statements)

References 16 publications

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“…Interestingly, different strains of S. thermophilus, a species phylogenetically closely related to S. salivarius, were shown by Kekkonen et al, as well as by some studies from our laboratory (data not shown), to induce high levels of IL-12 and IFN-␥ on PBMCs (34). Nevertheless, a preventive effect on inflammation in mice was observed with different S. thermophilus strains (35,36). Moreover, Ogita et al showed that S. thermophilus ST28 repressed IL-17 production and decreased the number of Th17 cells in stimulated murine splenocytes isolated from inflamed intestines (36).…”

Section: Discussionmentioning

confidence: 64%

Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Kaci

Goudercourt

Dennin

et al. 2014

Appl Environ Microbiol

160

122

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f Streptococcus salivarius is one of the first colonizers of the human oral cavity and gut after birth and therefore may contribute to the establishment of immune homeostasis and regulation of host inflammatory responses. The anti-inflammatory potential of S. salivarius was first evaluated in vitro on human intestinal epithelial cells and human peripheral blood mononuclear cells. We show that live S. salivarius strains inhibited in vitro the activation of the NF-B pathway on intestinal epithelial cells. We also demonstrate that the live S. salivarius JIM8772 strain significantly inhibited inflammation in severe and moderate colitis mouse models. These in vitro and in vivo anti-inflammatory properties were not found with heat-killed S. salivarius, suggesting a protective response exclusively with metabolically active bacteria.

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Section: Discussionmentioning

confidence: 64%

Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Kaci

Goudercourt

Dennin

et al. 2014

Appl Environ Microbiol

160

122

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“…We observed lowered activity of MPO, a protein released into the phagosome of neutrophils during the degranulation process, which when overexpressed can damage normal tissue and contribute to inflammation. Ito et al (2008) tested 2 S. thermophilus strains and found that only one, S. thermophilus YIT 2001, displaying high antioxidative activity, prevented oxidative injury to the colonic mucosa and improved the disease activity index in DSS-induced colitis in mice. We observed accelerated healing in the YC-administered mice, but the DSS-induced injury patterns and changes within the colonic mucosa and muscular compartment contributed to colonic dysregulation, resulting in immunological and microbiological alterations.…”

Section: Discussionmentioning

confidence: 99%

“…So far, despite the recognized nutritional and health benefits, traditional yogurt cultures in IBD were examined sporadically, and studies, although optimistic, have been conducted on single strains. Ito et al (2008) found that S. thermophilus YIT 2001 with antioxidative activity revealed an improving effect on dextran sulfate sodium (DSS)induced colitis. According to Takamura et al (2011), L. bulgaricus OLL1181 could activate the aryl hydrocarbon receptor pathway, which plays an important role in the regulation of the inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis

Wasilewska

Złotkowska

Wróblewska

2019

Journal of Dairy Science

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We investigated the yogurt starter cultures of Lactobacillus bulgaricus 151 and Streptococcus thermophilus MK-10 for their effect on the severity of experimental colitis, lymphocyte profile, and regulatory T-cell response. Colitis was induced in BALB/c mice via the administration of 3.5% dextran sulfate sodium salt (DSS) in drinking water for 6 d. Next, the mice were gavaged intragastrically with an active yogurt cultures (YC) mixture (~5 × 10 9 cfu/mouse per day) or saline (vehicle) for 8 d. Mice receiving DSS or saline alone served as positive and negative controls, respectively. The length of the colon, disease activity index, histological scores, myeloperoxidase activity, epitheliumassociated microbes, short-chain fatty acid profile, total IgA antibody-forming cells, CD3 + CD8 + , CD3 + CD4 + , CD3 + CD4 + CD25 + , CD3 + CD4 + CD25 + Foxp3 + T-cell subsets, and cytokine profiles (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and tumor necrosis factor) were examined after termination of the mice. Feeding mice with YC mixture reduced disease symptoms and modified intestinal microbiota and host inflammatory responsiveness to DSS. We observed limited weight loss and a decreased disease activity index score, lowered myeloperoxidase activity, and somewhat reduced damage of the intestine. The YC mixture upregulated the colon length, increased the amount and diversity of mucosaassociated microbes (enterobacteria, enterococci, and yeast), and decreased the concentration of putrefactive short-chain fatty acids in the cecal contents. It downregulated the input of cytotoxic CD3 + CD8 + T cells and CD3 + CD4 + CD25 + FoxP3 + regulatory T cells in Peyer's patches and enhanced CD3 + CD4 + CD25 + T cells in spleens and CD3 + CD4 + CD25 + FoxP3 + cells in peripheral blood mononuclear cells. Simultaneously, IgA antibody-forming cells were downregulated in mesenteric lymph nodes (MLN) and enhanced in spleens (SPL). The cultures mostly enhanced the production of cytokines tested in MLN and SPL, except for IL-6, which was downregulated in MLN. Interleukin-2 and IL-4 were the most upregulated in MLN, whereas IL-10, IL-4, IL-2, IFN-γ, and tumor necrosis factor were most upregulated in SPL. In serum, the YC mixture downregulated IFN-γ and clearly increased IL-2. Based on these results, we recognize the high anti-inflammatory and immunomodulatory potential of the L. bulgaricus 151 and S. thermophilus MK-10 set. The strains possess the ability to modulate the intestinal mucosal and systemic immune system toward both IgA production and induction of regulatory T cells, shifting Th1/Th2 balance.

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“…In addition to Bacilus subtilis , other probiotic strains such as Streptococcus thermophilus [ 17 , 18 , 19 , 20 ], Lactobacillus casei [ 21 , 22 , 23 , 24 , 25 ], Bifidobacterium breve [ 26 , 27 , 28 ] and Bifidobacterium animalis [ 29 , 30 , 31 ] have proven effective in reducing intestinal inflammation in pre-clinical models of IBDs, suggesting that a combination of Bacillus subtilis with these strains, by combining different metabolic and immune-modulatory effects [ 8 ], would exert an enhanced anti-inflammatory activity in comparison to the single strain formulation. Bacillus subtilis belong to the Bacillaceae family who are spore forming bacteria that are supposed to germinate in the living intestine unlike the Bifidocateria which are available as lyophilized preparations of vegetative cells [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Discovery of a Novel Multi-Strains Probiotic Formulation with Improved Efficacy toward Intestinal Inflammation

et al. 2020

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Dysbiosis is commonly detected in patients with inflammatory bowel disease (IBD), supporting the concept that a dysregulated immune reaction to bacterial antigens has a pathogenic role in the development of intestinal inflammation. In the present study, we have investigated the beneficial effects of a novel probiotic formulation assembled by combining four probiotics (Streptococcus thermophilus, Lactobacillus casei, Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis) with Bacillus subtilis, a Gram-positive bacterium, with extensive bio-applications. Mice rendered colitic by administration of TNBS or DSS were administered with Bacillus subtilis alone, Vivomixx® or the novel Five strains formulation. Vivomixx® attenuated the severity of inflammation and reduced the development of signs and symptoms of colitis in both models. Adding Bacillus subtilis to Vivomixx® improved the beneficial effects of the bacterial therapy. The novel Five strains formulation was as effective as Vivomixx® in reducing the development of signs and symptoms of colitis and reduced the expression of pro-inflammatory mediators including Il-6 and Tnf-α while increased the expression of Il-10 mRNA and the number of Treg. In summary, we have shown that a novel Five strains probiotics formulation exerts beneficial effects on two chemical models of colitis, establishing Bacillus subtilis as a probiotic in rodent models of inflammation.

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Preventive Effect ofStreptococcus thermophilusYIT 2001 on Dextran Sulfate Sodium-Induced Colitis in Mice

Cited by 12 publications

References 16 publications

Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis

Discovery of a Novel Multi-Strains Probiotic Formulation with Improved Efficacy toward Intestinal Inflammation

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