Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice

Yamashita, Maya; Matsumoto, Kurumi; Endo, Toshihiko; Ukibe, Ken; Hosoya, Tomohiro; Matsubara, Yumi; Nakagawa, Hiromi; Sakai, Fumihiko; Miyazaki, Tatsuya

doi:10.3389/fmicb.2017.01159

Cited by 53 publications

(41 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…25 Biogenics are easier to handle than In this study, we focused on LH2171, which has been used in food. The beneficial effects of LH2171, such as attenuation of autoimmune diseases, [26][27][28] have been demonstrated earlier. To evaluate the availability and functionality of LH2171, we investigated whether LH2171 upregulated the expression of antimicrobial peptides and subsequently suppressed the harmful bacteria.…”

Section: Discussionmentioning

confidence: 76%

Lactobacillus helveticus SBT2171 upregulates the expression of β‐defensin and ameliorates periodontal disease caused by Porphyromonas gingivalis

Kobatake

Kobayashi

Kabuki

et al. 2019

Microbiology and Immunology

View full text Add to dashboard Cite

Antimicrobial peptides play important roles in the innate immune system of various organisms, and they may also be considered to prevent the organisms from infections. In particular, β‐defensins, mainly produced in epithelial cells, are recognized as one of the major antimicrobial peptides in mammals, including humans. In this study, we showed that Lactobacillus helveticus SBT2171 (LH2171), one of the several species of lactic acid bacteria, upregulates the production of β‐defensins in oral epithelial cells in vitro. Moreover, LH2171 reduced the increase of proinflammatory cytokine expression, induced by Porphyromonas gingivalis stimulation, in gingival epithelial cells. These data suggested that LH2171 suppresses P. gingivalis‐induced inflammation by upregulating the expression of β‐defensins in gingival epithelial cells. We subsequently investigated the effects of LH2171 in vivo and revealed that β‐defensin expression was increased in the oral cavities of LH2171‐fed mice. Furthermore, LH2171 decreased alveolar bone loss, gingival inflammation, and amounts of P. gingivalis‐specific 16S ribosomal RNA in the gingiva of P. gingivalis‐inoculated mice. Taken together, our results showed that LH2171 upregulates the expression of β‐defensins in oral cavity, thereby decreasing the number of P. gingivalis consequently ameliorating the experimental periodontal disease.

show abstract

Section: Discussionmentioning

confidence: 76%

Lactobacillus helveticus SBT2171 upregulates the expression of β‐defensin and ameliorates periodontal disease caused by Porphyromonas gingivalis

Kobatake

Kobayashi

Kabuki

et al. 2019

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

“…For example, pre‐treatment with probiotics such as Lactobacillus rhamnosus (Gatej et al., ), Lactobacillus brevis CD2 (Maekawa & Hajishengallis, ) and Lactobacillus gasseri SBT2055 (Kobayashi et al., ) reduced bone loss and other catabolic changes of periodontal disease. Probiotics also protected mice form OVX‐induced bone loss (Britton et al., ; Ohlsson et al., ) and collagen‐induced arthritis (Yamashita et al., ). At a cellular level, probiotics increase the ratio of Treg‐Th17 cells and the ratio of anti‐inflammatory versus pro‐inflammatory cytokines (Dar, Pal, et al., ; Kwon et al., ).…”

Section: Preamblementioning

confidence: 99%

Osteoimmunology: Inflammatory osteolysis and regeneration of the alveolar bone

Gruber

2019

J Clinic Periodontology

113

View full text Add to dashboard Cite

Aim Osteoimmunology covers the cellular and molecular mechanisms responsible for inflammatory osteolysis that culminates in the degradation of alveolar bone. Osteoimmunology also focuses on the interplay of immune cells with bone cells during bone remodelling and regeneration. The aim of this review was to provide insights into how osteoimmunology affects alveolar bone health and disease. Method This review is based on a narrative approach to assemble mouse models that provide insights into the cellular and molecular mechanisms causing inflammatory osteolysis and on the impact of immune cells on alveolar bone regeneration. Results Mouse models have revealed the molecular pathways by which microbial and other factors activate immune cells that initiate an inflammatory response. The inflammation‐induced alveolar bone loss occurs with the concomitant suppression of bone formation. Mouse models also showed that immune cells contribute to the resolution of inflammation and bone regeneration, even though studies with a focus on alveolar socket healing are rare. Conclusions Considering that osteoimmunology is evolutionarily conserved, osteolysis removes the cause of inflammation by provoking tooth loss. The impact of immune cells on bone regeneration is presumably a way to reinitiate the developmental mechanisms of intramembranous and endochondral bone formation.

show abstract

“…Our previous study clarified that LH2171 significantly suppressed the proliferation of primary murine immune cells among 41 LAB strains of various species and decreased the production of lipopolysaccharide (LPS)-stimulated inflammatory cytokines from the immune cells in vitro [16]. In addition, LH2171 prevented the development of rheumatoid arthritis [17,18] and multiple sclerosis [19]-major autoimmune diseases-in a murine model in vivo. These reports suggested that LH2171 has beneficial immunoregulatory properties.…”

Section: Introductionmentioning

confidence: 99%

Anti-allergic effect of Lactobacillus helveticus SBT2171 on murine model of pollen allergy

Yamashita

Matsumoto

et al. 2019

FFHD

Self Cite

View full text Add to dashboard Cite

Background: We recently reported the suppressive effects of Lactobacillus helveticus SBT2171 (LH2171) on the proliferation and inflammatory cytokine production of primary murine immune cells in vitro. In this study, we investigated the effect of LH2171 on a murine pollen allergen- induced allergy model.Methods: Male BALB/c mice were fed a diet containing LH2171 (LH2171 group) or a diet not containing LH2171 (control group). Then, pollen allergy was induced by immunization with pollen extract emulsified in alum adjuvant and intranasal administration of pollen extract. To evaluate the anti-allergenic effect of LH2171, the antibody titer in plasma and expression of cytokines and transcription factors in immune tissues were analyzed by ELISA and qRT-PCR.Results: In the LH2171 group, the amounts of pollen-specific antibody and total IgE in plasma were significantly decreased compared with those in the control group. Proliferation and Th2 cytokine production were lower in the submandibular lymph node cells of the LH2171 group than in those of the control group. Moreover, in Peyer’s patches, the gene expression of Il10 and Foxp3 were increased in the LH2171 group.Conclusion: LH2171 has the ability to suppress production of allergy-related antibody and Th2 cytokines, which could be correlated with the induction of anti-inflammatory factors in the intestinal immune system.

show abstract

Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice

Cited by 53 publications

References 46 publications

Lactobacillus helveticus SBT2171 upregulates the expression of β‐defensin and ameliorates periodontal disease caused by Porphyromonas gingivalis

Lactobacillus helveticus SBT2171 upregulates the expression of β‐defensin and ameliorates periodontal disease caused by Porphyromonas gingivalis

Osteoimmunology: Inflammatory osteolysis and regeneration of the alveolar bone

Anti-allergic effect of Lactobacillus helveticus SBT2171 on murine model of pollen allergy

Contact Info

Product

Resources

About