Preventive Effect of Lactobacillus Plantarum CQPC10 on Activated Carbon Induced Constipation in Institute of Cancer Research (ICR) Mice

Abstract

Chinese Paocai is a traditional fermented food containing an abundance of beneficial microorganisms. In this study, the microorganisms in Szechwan Paocai were isolated and identified, and a strain of lactic acid bacteria (Lactobacillus plantarum CQPC10, LP-CQPC10) was found to exert an inhibitory effect on constipation. Microorganisms were isolated and identified via 16S rDNA. Activated carbon was used to induce constipation in a mouse model and the inhibitory effect of LP-CQPC10 on this induced constipation was investigated via both pathological sections and qPCR (quantitative polymerase chain reaction). A strain of Lactobacillus plantarum was identified and named LP-CQPC10. The obtained results showed that, as compared to the control group, LP-CQPC10 significantly inhibited the amount, weight, and water content of faeces. The defecation time of the first tarry stool was significantly shorter in LP-CQPC10 groups than in the control group. The activated carbon progradation rate was significantly higher when compared to the control group and the effectiveness was improved. LP-CQPC10 increased the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), AchE (acetylcholinesterase), SP (substance P), and VIP (vasoactive intestinal peptide), while decreasing the SS (somatostatin) level. Furthermore, it improved the GSH (glutathione) level and decreased the MPO (myeloperoxidase), MDA (malondialdehyde), and NO (nitric oxide) levels. The results of qPCR indicated that LP-CQPC10 significantly up-regulated the mRNA expression levels of c-Kit, SCF (stem cell factor), GDNF (glial cell-derived neurotrophic factor), eNOS (endothelial nitric oxide synthase), nNOS (neuronal nitric oxide synthase), and AQP3 (aquaporin-3), while down-regulating the expression levels of TRPV1 (transient receptor potential cation channel subfamily V member 1), iNOS (inducible nitric oxide synthase), and AQP9 (aquaporin-9). LP-CQPC10 showed a good inhibitory effect on experimentally induced constipation, and the obtained effectiveness is superior to that of Lactobacillus bulgaricus, indicating the better probiotic potential of this strain.

Lactobacillus Plantarum CQPC05 Isolated from Pickled Vegetables Inhibits Constipation in Mice

“…Through this study, it was observed that the bacterium has a good inhibitory effect on constipation in the laboratory, which proves that it is a probiotic microorganism. The results of this study are similar to those of previous studies [6,11,13,23], which suggest that Lactobacillus can inhibit constipation and be used as intestinal microorganisms. At the same time, the results of molecular biology further show that CQPC05 can regulate constipation by regulating the intensity of gene expression in the small intestine.…”

“…It has been proven that Lactobacillus possesses several functions. For example, Lactobacillus LF31 and Lactobacillus coryniformis have antioxidant effects [10], and Lactobacillus plantarum YS4 can prevent constipation in mice [11]. Our team investigated Szechwan-style pickled vegetables, and isolated and identified the microorganisms that they contain.…”

Preventive effect of Lactobacillus plantarum KSFY02 isolated from naturally fermented yogurt from Xinjiang, China, on d-galactose–induced oxidative aging in mice

“…However, under stress conditions, heme, hydrogen peroxide (H 2 O 2 ), endotoxin, heavy metals, UV radiation, and hyperoxia/ hypoxia can initiate HO-1 expression through Nrf2 and show strong antioxidant activity through scavenging free radicals such as hydrogen peroxide. The Nrf2/HO-1 pathway is widely involved in the protection of tissues and organs, including heart, brain, liver, kidney, and nervous system, from oxidative stress-induced damage, making it one of the most important endogenous protection systems in the body (Zhang et al, 2018). Studies have found that anthocyanin upregulates HO-1 through serine/threonine protein kinase (Akt) and extracellular regulation of protein kinase (ERK1/2)/Nrf2 signaling pathways to eliminate ROS, thereby exerting a protective effect on hepatotoxicity.…”

Preventive effect of flavonoids from Wushan Shencha (Malus doumeri leaves) on CCl4‐induced liver injury

“…After closure, the PVDF membrane was cleaned by 1 × TBST, and the first antibody was incubated at 25°C for 2 hr; the second antibody was incubated at 25°C after 5 times of cleaning by 1 × TBST. Finally, Supersignal West Pico PLUS (Thermo Fisher Science) was used to spray PVDF film and placed in iBright FL1000 (Thermo Fisher Science) for observation (Zhang et al, ).…”