Preventive effect of sanguinarine on intestinal injury in mice exposed to whole abdominal irradiation

Gu, Jia; Zhao, Lin; Chen, Yuzhong; Guo, Yaxin; Sun, Yue; Guo, Qing; Duan, Guangxin; Li, Chao; Tang, Zhibing; Zhang, Zixiang; Qin, Li–Qiang; Xu, Jia‐Ying

doi:10.1016/j.biopha.2021.112496

Cited by 18 publications

(12 citation statements)

References 49 publications

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“…The small intestine is the main site of animal digestion and absorption, and HS causes damage to the small intestinal mucosa [ 37 ]. The sanguinarine [ 38 ] and chelerythrine [ 39 , 40 ] in MCE can reduce intestinal inflammation and repair intestinal mucosal damage, which may partially explain the effect of MCE on reducing HS-induced performance damage in yellow-feathered broilers. The unaltered FCR may be due to the environment, among other reasons, thereby resulting in a lower ratio of the increased ADFI to meat product than the normal FCR.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Macleaya cordata Extract on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers Subjected to Chronic Heat Stress

Wang

Zhang

Huang

et al. 2022

Animals

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This study investigated the effect of dietary Macleaya cordata extract (MCE) supplementation on the growth performance, serum parameters, and intestinal microbiota of yellow-feather broilers under heat stress. A total of 216 yellow-feather broilers (28-days-old) were randomly allotted into three groups. A control group (CON) (24 ± 2 °C) and heat stress group (HS) (35 ± 2 °C) received a basal diet, and heat-stressed plus MCE groups (HS-MCE) (35 ± 2 °C) were fed the basal diet with 1000 mg/kg MCE for 14 consecutive days. The results revealed that MCE supplementation improved the final body weight, average daily feed intake, average daily gain, and spleen index when compared with the HS group (p < 0.05). In addition, MCE supplementation decreased (p < 0.05) the activities of aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and creatinine, and increased (p < 0.05) the glucose level and alkaline phosphatase activity in heat-stressed yellow-feathered broilers. Moreover, MCE treatment alleviated heat-stress-induced intestinal flora disturbances, decreased the Bacteroidota and Bacteroides relative abundances, and increased Firmicutes. A linear discriminant analysis effect size analysis found five differentially abundant taxa in the HS-MCE group, including Alistipes, Rikenellaceae, Mogibacterium, Butyrivibrio, and Lachnospira. These results suggest that MCE can alleviate HS-induced decline in growth performance by modulating blood biochemical markers and cecal flora composition in broilers.

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Section: Discussionmentioning

confidence: 99%

Effects of Dietary Macleaya cordata Extract on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers Subjected to Chronic Heat Stress

Wang

Zhang

Huang

et al. 2022

Animals

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“…The small intestine samples were harvested and fixed in 10% buffered formaldehyde– saline solution. Then, 4 μm sections of paraffin-embedded samples were stained with hematoxylin and eosin (H&E, Sigma-Aldrich), periodic acid-Schiff (PAS, Servicebio) and immune-histochemical reagents (IHC) as previously described (Gu et al, 2022). For IHC staining, deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784, Abcam, USA) or Ki67 (ab15580, Abcam, USA), then stained with biotinylated secondary antibodies.…”

Section: Methodsmentioning

confidence: 99%

“…Protein level was analyzed using Western blotting analysis as described previously (Gu et al, 2022). Briefly, total protein lysates were obtained from peritoneal macrophages using RIPA (Beyotime, China) buffer containing protease inhibitors at 4 °C for 30 min and centrifuged at 12, 000 g for 15min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The small intestine samples were harvested and fixed in 10% buffered formaldehyde-11 / 22 saline solution. Then, 4 μm sections of paraffin-embedded samples were stained with hematoxylin and eosin (H&E, Sigma-Aldrich), periodic acid-Schiff (PAS, Servicebio) and immune-histochemical reagents (IHC) as previously described (Gu et al, 2022).…”

Section: Histopathological Studiesmentioning

confidence: 99%

“…DNA extraction and 16S ribosomal DNA (rDNA) gene amplification were performed as described previously (Gu et al, 2022). The 16S rDNA V3-V4 gene was analyzed to evaluate the bacterial diversity using an Illumina MiSeq technology (Lc-Bio Technologies Co., Ltd., China).…”

Section: Bacterial Diversity Analysismentioning

confidence: 99%

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The radio-protective effects of (-)- Epigallocatechin-3-gallate (EGCG): regulating macrophage function in radiation-induced intestinal injury

Tang

et al. 2022

Preprint

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Accidental and medical radiation exposure may cause radiation-induced intestinal injury (RIII), a catastrophic disease requiring efficient therapies. (-)-Epigallocatechin-3-gallate (EGCG), a major constituent of green tea, has been shown to have potent biological activity as well as strong anti-inflammation effects. Here, we demonstrate that EGCG treatment not only protects mice against total body irradiation (TBI)-induced toxicity and weight loss but also alleviates whole abdominal irradiation (WAI)- induced intestinal injury. EGCG promotes proliferation and survival of intestinal stem cells, inhibits radiation-induced apoptosis and inflammation. At the same time, EGCG preserves the composition of the gut microbiota in WAI mice. In vitro, we demonstrate that EGCG regulates the release of radiation-induced inflammatory factors by inhibiting inflammatory pathways such as toll-like receptor signaling pathway in peritoneal macrophages. Mechanistically, the radioprotective effect of EGCG was likely attributable to its preservation on macrophages and the colonized gut microbiota composition, thus relieving the intestinal inflammation. EGCG provides a novel strategy to mitigate RIII and improve the prognosis of patients after radiotherapy.

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Cytotoxic Activity, Apoptosis Induction and Structure–Activity Relationship of 2‐Phenylphthalazin‐2‐ium Salts as Promising Antitumor Agents

et al. 2022

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Inspired by sanguinarine and chelerythrine, various 2‐phenylphthalazin‐2‐ium salts as simple analogues were designed according to the structure of natural products. On the basis of previous research, this study evaluated the inhibition activity of these compounds on tumor cells, apoptosis induction as well as their structure‐activity relationship (SAR). The results indicated that the compounds showed IC50 values of 1.21–14.77 μM against NB4 cells and 4.68–17.44 μM against MKN‐45 cells, respectively, superior to positive control cis‐platinum with IC50 values of 2.39 and 11.36 μM or their model compound chelerythrine with IC50 values of 1.49 and 12.70 μM. Acridine orange/ethidium bromide and ultrastructure observation of cells suggested that these compounds could induce apoptosis of tumor cells. Meanwhile, 2‐(3,4‐Dichlorophenyl)phthalazin‐2‐ium Bromide‐induced apoptosis was dependent on the excessive formation of ROS and amplified by the mitochondrial pathway. The preliminary SAR indicated that substitution pattern on the N‐aromatic ring remarkably influenced the cytotoxicity of the compounds. The trend showed that the presence of electron‐donating groups caused a significant improvement of the cytotoxicity. And in many cases that the 3’ site was the most advantageous substitution position for the enhancement of the cytotoxicity. Hence, the results demonstrated that the title compounds werea class of potential compounds for the development of novel antitumor drugs.

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Preventive effect of sanguinarine on intestinal injury in mice exposed to whole abdominal irradiation

Cited by 18 publications

References 49 publications

Effects of Dietary Macleaya cordata Extract on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers Subjected to Chronic Heat Stress

Effects of Dietary Macleaya cordata Extract on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers Subjected to Chronic Heat Stress

The radio-protective effects of (-)- Epigallocatechin-3-gallate (EGCG): regulating macrophage function in radiation-induced intestinal injury

Cytotoxic Activity, Apoptosis Induction and Structure–Activity Relationship of 2‐Phenylphthalazin‐2‐ium Salts as Promising Antitumor Agents

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