Preventive effects ofSalvia officinalisleaf extract on insulin resistance and inflammation in a model of high fat diet-induced obesity in mice that responds to rosiglitazone

Abstract

BackgroundSalvia officinalis (sage) is a native plant to the Mediterranean region and has been used for a long time in traditional medicine for various diseases. We investigated possible anti-diabetic, anti-inflammatory and anti-obesity effects of sage methanol (MetOH) extract in a nutritional mouse model of obesity, inflammation and insulin resistance, as well as its effects on lipolysis and lipogenesis in 3T3-L1 cells.MethodsDiet-induced obese (DIO) mice were treated for five weeks with sage methanol extract (100 and 400 mg kg−1/day bid), or rosiglitazone (3 mg kg−1/day bid), as a positive control. Energy expenditure, food intake, body weight, fat mass, liver glycogen and lipid content were evaluated. Blood glucose, and plasma levels of insulin, lipids leptin and pro- and anti-inflammatory cytokines were measured throughout the experiment. The effects of sage MetOH extract on lipolysis and lipogenesis were tested in vitro in 3T3-L1 cells.ResultsAfter two weeks of treatment, the lower dose of sage MetOH extract decreased blood glucose and plasma insulin levels during an oral glucose tolerance test (OGTT). An insulin tolerance test (ITT), performed at day 29 confirmed that sage improved insulin sensitivity. Groups treated with low dose sage and rosiglitazone showed very similar effects on OGTT and ITT. Sage also improved HOMA-IR, triglycerides and NEFA. Treatment with the low dose increased the plasma levels of the anti-inflammatory cytokines IL-2, IL-4 and IL-10 and reduced the plasma level of the pro-inflammatory cytokines IL-12, TNF-α, and KC/GRO. The GC analysis revealed the presence of two PPARs agonist in sage MetOH extract. In vitro, the extract reduced in a dose-related manner the accumulation of lipid droplets; however no effect on lipolysis was observed.ConclusionsSage MetOH extract at low dose exhibits similar effects to rosiglitazone. It improves insulin sensitivity, inhibits lipogenesis in adipocytes and reduces inflammation as judged by plasma cytokines. Sage presents an alternative to pharmaceuticals for the treatment of diabetes and associated inflammation.

Moringa oleifera leaf extract ameliorated high-fat diet-induced obesity, oxidative stress and disrupted metabolic hormones

“…ME showed similar effects to SIM and their combination. ME improved tissue insulin resistance, glucose levels and reduced body weight gain in HFD fed rats, which supports previous studies using polyphenol-rich plant extracts [28,29]. Insulin and glucose regulate leptin levels.…”

Anti-nephrotoxic and Antioxidant Efficiency of Rosmarinus Officinalis Extract Against Isoniazid®-Induced Nephropathy in Adult Male Albino Rats.

“…Favorably, administration of rosemary extract resulted in pronounced reduction in serum TNF-α and IL-1β, indicating its anti-inflammatory behaviors. This finding is in agreement with previous studies that reported the ability of rosemary to decrease circulating levels of TNF-α in hyperammonemic rats (Lopez-Nova et al, 2012;Ozbek, 2012;Bekker et al, 2016 andBen Khedher et al, 2018).…”

The protective effects ofSalvia officinalisessential oil compared to simvastatin against hyperlipidemia, liver, and kidney injuries in mice submitted to a high‐fat diet

“…Administration of either SEO or simvastatin to HFD‐treated mice was found to reduce dietary intake, as compared to the control group. Earlier, Ben Khedher et al () have shown that administration of Salvia officinalis leaf extract at a low dose (100 mg/kg body weight) to HFD fed mice provokes a reduced feed intake. According to these authors, this could be due to a decrease in the plasma levels of ghrelin, “the hunger hormone.” Indeed, the latter is a 28 amino acid peptide found in the stomach and the hypothalamus, as reported by Date et al ().…”

“…Administration of either SEO or simvastatin to HFDtreated mice was found to reduce dietary intake, as compared to the control group. Earlier, Ben Khedher et al (2018) have shown that administration of Salvia officinalis leaf extract at a low dose (100 mg/ kg body weight) to HFD fed mice provokes a reduced feed intake.…”