Preventive effects of nucleoprotein supplementation combined with intermittent loading on capillary regression induced by hindlimb unloading in rat soleus muscle

Hirayama, Yusuke; Nakanishi, Ryosuke; Maeshige, Noriaki; Fujino, Hidemi

doi:10.14814/phy2.13134

Cited by 12 publications

(14 citation statements)

References 33 publications

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“…Indeed, satellite cell proliferation which is required during muscle repair and may be important in muscle recovery after disuse (Alway, et al 2017b; Alway, et al 2011; Alway, et al 2014c; Alway, et al 2013; Brooks, et al 2018), is greater in fibers with a high capillarity to fiber ratio (Joanisse, et al 2018; Nederveen, et al 2018). Thus, it is possible that refraction of capillarity and therefore blood flow during unloading (Desplanches, et al 1990; Hirayama, et al 2017) is reversed during reloading, especially in the soleus fibers, which have greater capillarity than the gastrocnemius muscle fibers (Myrhage 1978). The dissimilar responses in type I fibers in the gastrocnemius and soleus muscles might be the result of non-uniform changes in vascularity throughout skeletal muscle arteriolar networks between soleus and gastrocnemius fibers (Laughlin and Roseguini 2008) leading to different potentials for the type I fibers in gastrocnemius and soleus muscles to receive humoral anabolic compounds (e.g., myokines) during reloading.…”

Section: Discussionmentioning

confidence: 99%

Effects of hindlimb suspension and reloading on gastrocnemius and soleus muscle mass and function in geriatric mice

Oliveira

Mohamed

Myers

et al. 2019

Experimental Gerontology

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Reloading of atrophied muscles after hindlimb suspension (HLS) can induce muscle injury and prolong recovery after disuse in old rats, especially in fast contracting muscles. Less is known about the responses in mice and whether fast and slow muscles from geriatric mice will respond in a similar fashion to HLS unloading and recovery (HLS+R). Furthermore, while slow muscles undergo atrophy with disuse, they typically are more resistant to sarcopenia than fast contracting muscles. Geriatric (28 mo. of age) male C57BL/6 mice were randomly placed into 3 groups. These included HLS for 14 days n=9, and HLS followed by 14 days of reloading recovery (HLS+R; n=9), or normal ambulatory cage controls (n=9). Control mice were not exposed to unloading. Electrically evoked maximal muscle function was assessed in vivo in anesthetized mice at baseline, after 14 days of HLS or HLS+R. As expected, HLS significantly reduced body weight, wet weight of gastrocnemius and soleus muscles and in vivo maximal force. There were no differences in vivo fatigability of the plantar flexor muscles and overall fiber size. There were only minor fiber type distribution and frequency distribution of fiber sizes that differ between HLS+R and control gastrocnemius and soleus muscles. Soleus muscle wet weight had recovered to control levels after reloading, but type I/IIA fibers in the soleus muscles were significantly smaller after HLS+R than control muscles. In contrast, gastrocnemius muscle wet weight did not recover to control levels after reloading. Plantar flexion muscle force (primarily influenced by the gastrocnemius muscles) did not recover in HLS+R conditions as compared to HLS conditions and both were lower than control force production Signaling for apoptosis, autophagy and anabolic markers were not different between control and HLS+R gastrocnemius and soleus muscles in geriatric mice. These results suggest that molecular signaling does not explain attenuated ability to regain muscle wet weight, fiber size or muscle force production after HLS in geriatric mice. It is possible that fluid shifts, reduced blood flow, or shortened muscle fibers which failed to regain control lengths contributed to the attenuation of muscle wet weight after HLS and reloading and this affected force production. Further work is needed to determine if altered/loss of neural activity contributed to the inability of geriatric mice to regain gastrocnemius muscle weight and function after HLS and reloading.

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Section: Discussionmentioning

confidence: 99%

Effects of hindlimb suspension and reloading on gastrocnemius and soleus muscle mass and function in geriatric mice

Oliveira

Mohamed

Myers

et al. 2019

Experimental Gerontology

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“…Chronic neuromuscular inactivity induces quantitative and qualitative changes in skeletal muscles. Muscle disuse results in morphological and functional alterations such as muscle atrophy [1], slow-to-fast fiber switching [2,3], capillary regression [4][5][6], and mitochondrial dysfunction [7] within the muscles, particularly in muscles predominantly comprising slow oxidative fibers such as the soleus muscle.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, various strategies such as antioxidant supplementation have been formulated for treating oxidative stress. Emerging studies suggest that providing antioxidant supplementation during HU counteracts qualitative alterations such as fibertype transition and reduced mitochondrial activity [5,14,15]. However, the positive effect of antioxidant treatment on muscle fiber size is still controversial.…”

Section: Introductionmentioning

confidence: 99%

Effects of astaxanthin supplementation and electrical stimulation on muscle atrophy and decreased oxidative capacity in soleus muscle during hindlimb unloading in rats

et al. 2019

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The effects of a combination of the antioxidant astaxanthin (AX) and electrical stimulation (ES) on muscle mass and mitochondrial oxidative capacity were investigated in the soleus muscle of hindlimb unloaded rats. Five groups of male Sprague-Dawley rats were used; control, 1-week hindlimb unloading (HU), HU + AX, HU + ES, and HU + AX + ES. Respective rats in the AX groups received 50-mg/kg AX twice daily during HU. Calf muscles of rats in the ES groups were electrically stimulated for 240 s/day during HU. One-week HU decreased muscle mass along with decreased FoxO3a phosphorylation and increased ubiquitinated proteins expressions, decreased oxidative enzymatic activity accompanied with decline in PGC-1α protein expression, and increased reactive oxygen species production. However, the combination treatment could synergistically attenuate/suppress all HU-related changes, suggesting protective effects on muscle atrophy and decreased muscle oxidative capacity due to chronic neuromuscular inactivity.

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“…VEGF, one of the major pro‐angiogenic factors, is regulated by changes in the blood flow volume 5 . However, the repeated change in the blood flow volume induced VEGF response and caused a gradual reduction in VEGF levels, finally returning to the control level after a week 52,53 . The present study involved two weeks of exercise and 10 days of normal activity after the exercise period.…”

Section: Discussionmentioning

confidence: 95%

Differential effects of pre‐exercise on cancer cachexia‐induced muscle atrophy in fast‐ and slow‐twitch muscles

et al. 2020

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We hypothesized that pre-exercise may effectively prevent cancer cachexia-induced muscle atrophy in both fast-and slow-twitch muscle types. Additionally, the fasttwitch muscle may be more affected by cancer cachexia than slow-twitch muscle. This study aimed to evaluate the effects of pre-exercise on cancer cachexia-induced atrophy and on atrophy in fast-and slow-twitch muscles. Twelve male Wistar rats were randomly divided into sedentary and exercise groups, and another 24 rats were randomly divided into control, pre-exercise, cancer cachexia induced by intraperitoneal injections of ascites hepatoma AH130 cells, and pre-exercise plus cancer cachexia groups. We analyzed changes in muscle mass and in gene and protein expression levels of major regulators and indicators of muscle protein degradation and synthesis pathways, angiogenic factors, and mitochondrial function in both the plantaris and soleus muscles. Pre-exercise inhibited muscle mass loss, rescued protein synthesis, prevented capillary regression, and suppressed hypoxia in the plantaris and soleus muscles. Pre-exercise inhibited mitochondrial dysfunction differently in fastand slow-twitch muscles. These results suggested that pre-exercise has the potential to inhibit cancer-cachexia-induced muscle atrophy in both fast-and slow-twitch muscles. Furthermore, the different progressions of cancer-cachexia-induced muscle atrophy in fast-and slow-twitch muscles are related to differences in mitochondrial function.

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Preventive effects of nucleoprotein supplementation combined with intermittent loading on capillary regression induced by hindlimb unloading in rat soleus muscle

Cited by 12 publications

References 33 publications

Effects of hindlimb suspension and reloading on gastrocnemius and soleus muscle mass and function in geriatric mice

Effects of hindlimb suspension and reloading on gastrocnemius and soleus muscle mass and function in geriatric mice

Effects of astaxanthin supplementation and electrical stimulation on muscle atrophy and decreased oxidative capacity in soleus muscle during hindlimb unloading in rats

Differential effects of pre‐exercise on cancer cachexia‐induced muscle atrophy in fast‐ and slow‐twitch muscles

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