Prevotella hominis sp. nov., isolated from human faeces

Abstract

A strictly anaerobic predominant bacterium, designated as strain gm001T, was isolated from a freshly voided faecal sample collected from a healthy Taiwanese adult. Cells were Gram-stain-negative rods, non-motile and non-spore-forming. Strain gm001T was identified as a member of the genus Prevotella , and a comparison of 16S rRNA and hsp60 gene sequences revealed sequence similarities of 98.5 and 93.3 %, respectively, demonstrating that it was most closely related to the type strain of Prevotella copri . Phylogenomic tree analysis indicated that the gm001T cluster is an independent lineage of P. copri DSM 18205T. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between strain gm001T and P. copri DSM 18205T were 80.9, 28.6 and 83.8 %, respectively, which were clearly lower than the species delineation thresholds. The species-specific genes of this novel species were also identified on the basis of pan-genomic analysis. The predominant menaquinones were MK-11 and MK-12, and the predominant fatty acids were anteiso-C15 : 0, C15 : 0 and iso-C15 : 0. Acetate and succinate were produced from glucose as metabolic end products. Taken together, the results indicate that strain gm001T represents a novel species of the genus Prevotella , for which the name Prevotella hominis sp. nov. is proposed. The type strain is gm001T (=BCRC 81118T=JCM 33280T).

Extension of the Segatella copri complex to 13 species with distinct large extrachromosomal elements and associations with host conditions

“…In the phylogeny built for these 165 SGBs (see STAR Methods ), we identified ScC as the minimal monophyletic subtree that comprised all reference genomes labeled as S. copri in the NCBI GenBank database 30 and resulted to be clearly distinct from other species ( Figure S1 ). The expanded ScC 20 , 21 comprised 9 additional species-level clades in addition to the four clades previously described (clades A–D), 20 revealing a total of 13 distinct species (including the recently described species Segatella hominis , formerly Prevotella hominis , 40 as clade E; Figure 1 B).…”

Statistical study of the feeding factor in samples of Iberian pig microbiome

“…Using the non-parametric Wilcoxon test it is determined that the species that generates the greatest signi cant differences between all levels of feeding is also Prevotella hominis (Liou et al 2020). Being able to obtain this species through the two different methods used re ects robustness in this study.…”

The Evaluation of Bacterial Abundance and Functional Potentials in the Three Major Watersheds, Located in the Hot Spring Zone of the Tatun Volcano Group Basin, Taiwan

“…Prevotella was higher in the HGC, which is a human gut bacterium, and has been previously isolated from human feces [ 56 ]. The HGC is a popular spa area in Taiwan [ 57 ]; thus, anthropogenic interventions in the creek may be the key reason for the abundance of Prevotella . In addition, Prevotella was less tolerant to dissolved oxygen content [ 58 ].…”