Prevotella micans sp. nov., isolated from the human oral cavity

Downes, Julia; Liu, Minchuan; Könönen, Eija; Wade, William

doi:10.1099/ijs.0.002337-0

Cited by 25 publications

(5 citation statements)

References 11 publications

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“…The DNA G+C content of the type strain is 41 mol%. Holdeman et al, 1982); 4, P. dentalis (Haapasalo et al, 1986); 5, P. oris (Holdeman et al, 1982); 6, P. salivae (Sakamoto et al, 2004); 7, P. maculosa (Downes et al, 2007); 8, P. multisaccharivorax (Sakamoto et al, 2005b); 9, P. oralis (Loesche et al, 1964;Watabe et al, 1983); 10, P. baroniae (Downes et al, 2005); 11, P. micans (Downes et al, 2009); 12, P. buccalis (Shah & Collins, 1981); 13, P. loescheii (Holdeman and Johnson, 1982); 14, P. veroralis (Watabe et al, 1983); 15, P. multiformis (Sakamoto et al, 2005a); 16, P. nanceiensis (Alauzet et al, 2007); 17, P. denticola (Shah & Collins, 1982); 18, P. melaninogenica (Holdeman and Johnson, 1982); 19, P. histicola (Downes et al, 2008); 20, P. shahii (Sakamoto et al, 2004); 21, P. oulorum (Shah et al, 1985); 22, P. enoeca (Moore et al, 1994); 23, P. pleuritidis (Sakamoto et al, 2007); 24, P. tannerae (Moore et al, 1994); 25, P. intermedia (Johnson and Holdeman, 1983); 26, P. nigrescens (Shah & Gharbia, 1992); 27, P. marshii (Downes et al, 2005); 28, P. pallens (Könönen et al, 1998). +, Positive; 2, negative; W, weakly positive; V, variable.…”

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confidence: 99%

Prevotella fusca sp. nov. and Prevotella scopos sp. nov., isolated from the human oral cavity

Downes

Wade

2011

International Journal of Systematic and Evolutionary Microbiology

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Two strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to belong to two separate taxa. Phylogenetic analysis of full-length 16S rRNA gene sequences showed that the strains were both related to, but distinct from, the type strain of Prevotella melaninogenica. Two novel species, Prevotella fusca sp. nov. and Prevotella scopos sp. nov., are proposed to accommodate these strains. Both strains were saccharolytic and produced acetic and succinic acids, with lesser amounts of lactic and isovaleric acids, as end products of fermentation, and both were sensitive to 20 % bile. The principal cellular long-chain fatty acids of both strains were ai-C 15 : 0 , 3-OH i-C 17 : 0 , 3-OH C 16 : 0 , i-C 15 : 0 and C 16 : 0 . The DNA G+C contents of the type strains of Prevotella fusca (W1435 T 5DSM 22504 T 5CCUG 57946 T ) and Prevotella scopos (W2052 T 5DSM 22613 T 5CCUG 57945 T ) were 43 and 41 mol%, respectively. The two species could be differentiated by gelatin hydrolysis, cellobiose and ribose fermentation, and production of b-glucosidase.Members of the genus Prevotella are among the species most frequently isolated from the human oral cavity. In this study, two strains provisionally identified as belonging to the genus Prevotella by 16S rRNA gene partial sequence analysis, but distinct from current species, were subjected to a range of phenotypic and genetic tests. Strain W1435T was isolated from subgingival plaque in an individual with periodontitis and strain W2052T was from the exudate associated with a failing dental implant. Prevotella melaninogenica ATCC 25845 T was obtained from the ATCC. Strains were grown at 37 uC on Fastidious Anaerobe Agar (FAA; LabM) supplemented with 5 % horse blood under anaerobic conditions (80 % N 2 , 10 % H 2 , 10 % CO 2 ) in an anaerobic workstation (Don Whitley Scientific). Colonial morphologies were determined by using a dissecting microscope after 4 days of incubation on FAA. Cellular morphology was recorded after Gramstaining of smears prepared from 2-day-old FAA plate cultures. Hanging-drop preparations of 18 h cultures in peptone-yeast extract-glucose (PYG) broth were examined for cellular motility by phase-contrast microscopy. The range and optimum temperatures for growth were determined after 48 h of incubation in pre-reduced PYG broth that had been dispensed into anaerobically sterilized (pre-reduced, anaerobically sterilized; PRAS) tubes in an anaerobic workstation (Don Whitley Scientific). The range and optimum pH for growth were determined in peptoneyeast extract (PY) broth (Holdeman et al., 1977) incubated at 35 u C for 48 h with initial pH values obtained by adding HCl (0.2 M) or Na 2 CO 3 (10 %, w/v) to the PY broth.Biochemical and physiological tests were performed using standard methods (Holdeman et al., 1977; JousimiesSomer et al., 2002). Fermentation tests were performed using PRAS sugars prepared in-house in an anaerobic workstation (Holdeman et al., 1977)...

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confidence: 99%

Prevotella fusca sp. nov. and Prevotella scopos sp. nov., isolated from the human oral cavity

Downes

Wade

2011

International Journal of Systematic and Evolutionary Microbiology

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“…For example, there are a multitude of CL/P-prone microbes associated with caries, including Bifidobacterium dentium , Lactobacillus spp., a Neisseria sp., Rothia dentocariosa , Scardovia wiggsiae , Streptococcus cristatus , Streptococcus mutans , and Streptococcus salivarius ( Kressirer et al., 2017 ; Inquimbert et al., 2019 ; Xiao et al., 2021 ), while Anaeroglobus geminatus and Shuttleworthia satelles have been found to be more prevalent in patients with caries ( Wang et al., 2019 ). Indeed, several CL/P-prone microbes, such as P. marshii , P. micans , S. wiggsiae, Selenomonas artemidis , and S. satelles , produce acidic byproducts from sugar metabolism, contributing to the development of an acidic oral environment, and, potentially, caries ( Moore et al., 1987 ; Downes et al., 2002 ; Downes et al., 2005 ; Downes et al., 2009 ; Kressirer et al., 2017 ). Moreover, Catonella morbi has been implicated in a more serious affliction of the teeth, primary endodontic infections, while Propionibacterium has been implicated in secondary endodontic infections and lesions ( Siqueira and Rôças, 2006 ; Dioguardi et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

Gershater

Liu

Xue

et al. 2023

Front. Cell. Infect. Microbiol.

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Orofacial cleft disorders, including cleft lip and/or palate (CL/P), are one of the most frequently-occurring congenital disorders worldwide. The health issues of patients with CL/P encompass far more than just their anatomic anomaly, as patients with CL/P are prone to having a high incidence of infectious diseases. While it has been previously established that the oral microbiome of patients with CL/P differs from that of unaffected patients, the exact nature of this variance, including the relevant bacterial species, has not been fully elucidated; likewise, examination of anatomic locations besides the cleft site has been neglected. Here, we intended to provide a comprehensive review to highlight the significant microbiota differences between CL/P patients and healthy subjects in various anatomic locations, including the teeth inside and adjacent to the cleft, oral cavity, nasal cavity, pharynx, and ear, as well as bodily fluids, secretions, and excretions. A number of bacterial and fungal species that have been proven to be pathogenic were found to be prevalently and/or specifically detected in CL/P patients, which can benefit the development of CL/P-specific microbiota management strategies.

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“…The genus Prevotella consists of moderately saccharolytic, bile-sensitive, predominantly oral species of formerly classified in the genus Bacteroides [39]. Novel Prevotella species, P. micans and P. nanceiensis, have been isolated not only from the oral cavity, but also from other sites of the body [45,46]. The nonpigmented former Mitsuokella dentalis and Hallella seregens, which are associated with endodontic and other oral infections, have been reclassified as Prevotella dentalis [47].…”

Section: ) Prevotella and Porphyromonasmentioning

confidence: 99%

Update on the Taxonomy of Clinically Important Anaerobic Bacteria

Kim

2022

Korean J Clin Lab Sci

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The taxonomy of bacteria in the field of clinical microbiology is in a state of constant flux. A large-scale revamping of the classification and nomenclature of anaerobic bacteria has taken place over the past few decades, mainly due to advances in molecular techniques such as 16S rRNA and whole genome sequencing (WGS). New genera and species have been added, and existing genera and species have been reclassified or renamed. A major role of the clinical microbiological laboratories (CMLs) is the accurate identification (ID) and appropriate antimicrobial susceptibility testing (AST) for clinically important bacteria, and rapid reporting and communication of the same to the clinician. Taxonomic changes in anaerobic bacteria could potentially affect the choice of appropriate antimicrobial agents and the antimicrobial breakpoints to use. Furthermore, current taxonomy is important to prevent treatment failures of emerging pathogenic anaerobes with antimicrobial resistance. Therefore, CMLs should periodically update themselves on the changes in the taxonomy of anaerobic bacteria and suitably inform clinicians of these changes for optimum patient care. This article presents an update on the taxonomy of clinically important anaerobic bacteria, together with the previous names or synonyms. This taxonomy update can help guide antimicrobial therapy for anaerobic bacterial infections and prevent treatment failure and can be a useful tool for both CMLs and clinicians.

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Prevotella micans sp. nov., isolated from the human oral cavity

Cited by 25 publications

References 11 publications

Prevotella fusca sp. nov. and Prevotella scopos sp. nov., isolated from the human oral cavity

Prevotella fusca sp. nov. and Prevotella scopos sp. nov., isolated from the human oral cavity

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

Update on the Taxonomy of Clinically Important Anaerobic Bacteria

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