Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque

Abstract

Six bacterial strains isolated from the human oral cavity, PPPA16, PPPA20 T , PPPA24, PPPA31, EPPA6 and EPPA7, were characterized by determining phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the isolates represented the same species of the genus Prevotella. The strains were related to Prevotella dentalis with about 89 % similarity. In addition, the isolates were related to Prevotella sp. oral clone IDR-CEC-0032, which is a representative of the numerically dominant cluster VI in carious dentine lesions [Nadkarni et al. (2004). J Clin Microbiol 42, 5238-5244], with about 99 % similarity. The strains were obligately anaerobic, non-pigmenting, non-spore-forming, non-motile, Gram-negative rods. The isolates could be differentiated from other Prevotella species by D-mannitol, D-melezitose, D-sorbitol and D-trehalose fermentation in API 20A tests. The cellular fatty acid composition of strains PPPA16, PPPA20T , PPPA24, PPPA31, EPPA6 and EPPA7 was significantly different from that of other Prevotella species. Compared with other Prevotella species, only these six strains contained dimethyl acetals. The major menaquinones of the clinical isolates were MK-12 and MK-13, whereas the major menaquinones of other Prevotella species were MK-10 and MK-11. On the basis of these data, a novel Prevotella species, Prevotella multisaccharivorax sp. nov., is proposed, with PPPA20 T (=JCM 12954 T =DSM 17128 T ) as the type strain.

Prevotella colorans sp. nov., isolated from a human wound

“…This value lies within the range reported for the species in the genus Prevotella (39-60 mol%) (Avgustin, 1997, Willems & Collins, 1995. Compared with the phylogenetically most closely related species, strain A1336 T can clearly be distinguished by its genomic DNA G+C content from P. bergensis [48 mol%; (Downes et al, 2006)] and P. multisaccharivorax [49.9 mol%; (Sakamoto et al, 2005)]. …”

Genome-Based Taxonomic Classification of Bacteroidetes

“…The description is as given by Sakamoto et al (2005) with the following modification. The genomic G+C content of the type strain is 48.3%.…”

Non-contiguous finished genome sequence of the opportunistic oral pathogen Prevotella multisaccharivorax type strain (PPPA20T)

“…The organism is described as non-motile and only four genes associated with motility were identified in the genome (see below). P. multisaccharivorax grows well at 37°C, is strictly anaerobic, chemoorganotrophic and is able to ferment cellobiose, glucose, glycerol, lactose, maltose, mannose, melezitose, raffinose, rhamnose, sorbitol, sucrose, trehalose and xylose [2]. Acid production from arabinose and salicin is variable.…”

“…Currently, there are about 50 species placed in the genus Prevotella [1]. The species epithet is derived from the Latin adjective multus meaning ‘many/much’, the Latin noun saccharum meaning ‘sugar’ and the Latin adjective vorax meaning ‘liking to eat’ referring to the metabolic properties of the species to digest a variety of carbohydrates [2]. P. multisaccharivorax strain PPPA20 T is considered to be an opportunistic pathogen and was isolated from subgingival plaque from a patient with chronic periodontitis.…”

“…P. multisaccharivorax strain PPPA20 T is considered to be an opportunistic pathogen and was isolated from subgingival plaque from a patient with chronic periodontitis. Additionally, five more strains isolated from the human oral cavity were placed in the species P. multisaccharivorax [2]. Using non-culture techniques on sites affected by endodontic and periodontal diseases, a large number of sequences have been found that belong to Prevotella and Prevotella -like bacteria.…”