20!Hunting mode, the distinct set of behavioural strategies that a predator employs while 21! hunting, can be an important determinant of the prey organism's behavioural response.
22!However, few studies have considered how a predator's hunting mode influences anti-23! predatory behaviours of a prey species. Here we document the influence of active hunters 24! (zebra jumping spiders, Salticus scenicus) and ambush predators (Chinese praying 25! mantids, Tenodera aridifolia sinensis) on the capture deterrence anti-predatory 26! behavioural repertoire of the model organism, Drosophila melanogaster. We 27! hypothesized that D. melanogaster would reduce overall locomotory activity in the 28! presence of ambush predators, and increase activity with active hunters. First we 29! observed and described the behavioural repertoire of D. melanogaster in the presence of 30! the predators. We documented three previously undescribed behaviours-abdominal 31! lifting, stopping and retreat-which were performed at higher frequency by D. 32! melanogaster in the presence of predators, and may aid in capture deterrence. Consistent 33! with our predictions, we observed an increase in the overall activity of D. melanogaster 34! in the presence of jumping spiders (active hunter). However, counter to our prediction, 35! mantids (ambush hunter) had only a modest influence on activity. We also observed 36! considerable intra and inter-individual variation in response to both predator types. Given 37! these new insights into Drosophila behaviour, and with the genetic tools available, 38! dissecting the molecular mechanisms of anti-predator behaviours may now be feasible in 39! this system.