Pri‐miR‐144 rs9279 is associated with type 2 diabetes and regulation of stress response

Yan, Yuxiang; Xiao, Han; Zhang, Jie; Wang, Shuo; Dong, Jing; Wu, Lijuan

doi:10.1002/jcp.29883

Cited by 8 publications

(9 citation statements)

References 32 publications

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“…MirSNP predicts that rs873601-A enhances the binding activity of miR-4715-3p, and rs873601-A decrease the binding activity of miR-4715-3p 29 (Supplementary Figure 3), the polymorphism of target site among in target gene, miRNA or lncRNA could potentially alter stability of binding. 30,31 Together with these, our results confirmed that the rs873601 polymorphism of XPG is associated with susceptibility and biological implications to lung cancer.…”

Section: Discussionsupporting

confidence: 82%

XPG is Modulated by miR-4715-3p and rs873601 Genotypes in Lung Cancer

Yao

Lyu

et al. 2021

CMAR

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Objective: XPG (Xeroderma pigmentosum group G, XPG), a single strand-specific DNA endonuclease in the nucleotide excision repair pathway, has been implicated in lung cancer. Potentially functional rs873601 in XPG is consistently associated with gastrointestinal cancer, and miR-4715-3p, targeting 3UTR of XPG, also influences the process of gastrointestinal carcinogenesis, however, the relationships between XPG and miR-4715-3p and rs873601 in lung cancer have not been elucidated. Methods: A case-control study included 264 lung cancer patients and 264 cancer-free healthy controls and was designed to determine the relationships between rs873601 and lung cancer and the effect of miR-4715-3p on XPG expression in lung cancer. Fifty matched cases and controls were randomly selected from the lung cancer and control groups to assess the relationships between the expression levels of miR-4715-3p and XPG determined by using qRT-PCR. The association of rs873601 with lung cancer was analyzed by mass spectrometry, and function prediciton of rs873601 genotypes explored by web-based bioinformatics. Results: miR-4715-3p in the lung cancer group was significantly increased compared with that in the control group (P = 0.011), upregulation of miR-4715-3p correlated with an increase in XPG mRNA (r = 0.399, P <0.05) in the lung cancer group. The AA genotype was associated with increased risk of lung cancer compared with the AG and GG genotypes of rs873601 (AG vs AA: OR = 0.231, 95% CI: 0.155-0.345, P <0.001 GG vs AA: OR = 0.300, 95% CI: 0.131-0.719, P = 0.003). The genetic association remained significant after adjustment for age, sex, smoking, and drinking, and rs873601-AA was associated with an increase in XPG mRNA in the lung cancer group. The results of web-based bioinformatics analysis indicated rs873601 genotypes might change XPG-RNA stability and bindability between XPG and miR-4715-3p. Conclusion:Our data characterized that miR-4715-3p and rs873601 genotypes modified XPG expression in lung cancer. These findings may help to elucidate the mechanisms governing lung cancer.

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Section: Discussionsupporting

confidence: 82%

XPG is Modulated by miR-4715-3p and rs873601 Genotypes in Lung Cancer

Yao

Lyu

et al. 2021

CMAR

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“…In our previous study, NR3C1 was proved to be a direct target gene of miR-144-3p by luciferase assay ( 24 ). miR-144 has been identified as a stressor-responsive miRNA by several studies.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, let-7b, miR-142, miR-144, and miR-29a have been identified as neuroendocrine stress response-related biomarkers for T2D and IR ( 23 ). NR3C1 was further identified as a target gene of miR-144 ( 24 ). These evidences suggest that stress-related miRNAs may be potential novel targets for treatment of stress-related disorders.…”

Section: Introductionmentioning

confidence: 99%

hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p

Yan

Xiao

et al. 2022

Front. Endocrinol.

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IntroductionChronic stress plays an important role in the development of type 2 diabetes (T2D). Circular RNAs (circRNAs) play significant roles in regulating the pathogenesis of diseases by regulating gene expression. The aim of the present study was to identify the association between hsa_circ_0111707 and stress-related T2D.MethodsThe present study was performed based on a three-part design. The association between hsa_circ_0111707 in peripheral blood mononuclear cells (PBMCs) and T2D and stress-related variables were assessed in a cross-sectional study. The causal relationship of hsa_circ_0111707 on T2D was further investigated in a nested case-control study. miR-144-3p as the miRNA target of hsa_circ_0111707 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.ResultsThe relative expression of hsa_circ_0111707 was significantly lower in the T2D and impaired fasting glucose (IFG) cases in comparison with controls. The hsa_circ_0111707 expression was significantly negatively correlated with miR-144-3p expression and plasma cortisol concentration and positively correlated with NR3C1 expression. In addition, hsa_circ_0111707 expression was negatively correlated with scores of “demands at work” and “insecurity at work” of Copenhagen Psychosocial Questionnaire (COPSOQ). Decreased hsa_circ_0111707 expression was associated with increased risk of T2D development. Functional analysis demonstrated that hsa_circ_0111707 functions as a sponge for miR-144-3p.Conclusionhsa_circ_0111707 is associated with risk of T2D development via sponging miR-144-3p. hsa_circ_0111707 in PBMCs can be considered a potential biomarker of stress-related T2D.

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“…There is now ample evidence in a range of different diseases including pre-eclampsia (Li et al, 2021), type 2 diabetes (Yan et al, 2021), lung fibrosis after lung transplantation (Wang et al, 2020), non-alcoholic fatty liver disease (López-Sánchez et al, 2021), liver cancer (Ghafouri-Fard et al, 2021) and multiple sclerosis (Shareef et al, 2021), that polymorphisms in the relevant miRNA can confer susceptibility and determine the effectiveness of suppression of gene expression. In addition, polymorphisms in the miRNA binding domain of target gene regions play a role in diseases such as colorectal cancer where SNPs in the solute carrier transporter gene predict clinical outcome in colorectal cancer (Bendova et al, 2020) and the KRT81 gene in breast cancer (Sha et al, 2020).…”

Section: Micro-rna Moleculesmentioning

confidence: 99%

The importance of establishing genetic phase in clinical medicine

Tait

2021

Int J Immunogenetics

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Haplotyping or determination of genetic phase has always played a pivotal role in MHC (HLA studies) both in helping to understand inheritance patterns in diseases such as type 1 diabetes (T1D) and in ensuring better matching in transplantation scenarios such as haematopoietic stem cell transplantation (HSCT), using donors genetically related to the patient. In recent years the need to establish genetic phase in a number of clinical scenarios has become apparent. These include:1. Genetic phasing for hematopoietic stem cell transplants using unrelated donors, where the HLA haplotypes are not known but where haplotype-matched recipients fare better clinically than allele matched, but haplotype mismatched patients.2. The use of checkpoint inhibitors is one of the most innovative and exciting developments in cancer treatment in years. An example is the use of the monoclonal ipilimumab to block the CTLA-4 receptor which is known to contain polymorphic sites.Until the phase of these polymorphisms is known it will not be possible to determine how effectively this monoclonal will perform in individual patients.3. The role of miRNA single strand molecules and their effect on gene expression. Thousands of non-coding genes have been identified and have been shown to be polymorphic, as have their target genes. Genetic phasing of polymorphism both in the miRNA source genes and their targets is clearly a fertile area of research 4. In areas such a drug metabolism where the polymorphic family of CYP genes is responsible for the metabolism of the majority of prescription drugs, determining phase of SNPs is critical to understanding drug metabolism and efficacy. 5. In multigenic disease studies combinations of single nucleotide polymorphisms (SNPs) in participating genes require accurate phasing in order to fully appreciate their role in the disease process. In addition, the level of expression of genes (point 3) is also important in understanding disease processes at the functional level. This review outlines the techniques that are currently available for approximating phase and discusses the clinical relevance of establishing genetic phase in areas of clinical medicine outlined in points 1-3.

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Pri‐miR‐144 rs9279 is associated with type 2 diabetes and regulation of stress response

Cited by 8 publications

References 32 publications

XPG is Modulated by miR-4715-3p and rs873601 Genotypes in Lung Cancer

XPG is Modulated by miR-4715-3p and rs873601 Genotypes in Lung Cancer

hsa_circ_0111707 Is Associated With Risk of Stress-Related Type 2 Diabetes via Sponging miR-144-3p

The importance of establishing genetic phase in clinical medicine

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