Consumers perceive the level of organic food prices as the major obstacle to increasing purchases. The main objective of the presented study is to recognize the differences between the prices of organic and non-organic food (so-called price premiums) in the Polish organic food market. Consequently, in order to determine these differences, prices of organic and non-organic food were noted in 45 retail outlets providing both types of food products. The investigation was performed within the Poznań agglomeration for nine months, from October 2022 to June 2023. The research showed that organic food price premiums ranged between 35% and over 270%. The lowest premiums were observed in tomato passata, cereal and dairy products, and eggs and olive oil. The highest occurred in chocolate, tea, juices, rapeseed oil, and chicken. Compared to previous studies, premiums observed in the conducted research, although high, have decreased for most investigated products. Moreover, the differences in price premiums were noted depending on the outlet type—they were lower in supermarket and hypermarket chains than in small retail shops. It should be highlighted that measures to maintain organic market development should be carried out with particular emphasis on the continued participation of retail chains in the distribution of organic food and the increased participation of large companies in processing organic food.