Priestia sp. LWS1 Is a Selenium-Resistant Plant Growth-Promoting Bacterium That Can Enhance Plant Growth and Selenium Accumulation in Oryza sativa L.

Abstract

Selenium (Se) is essential for the basic functions of life, but the low daily intake of Se urges us to find reliable ways to increase food Se content. Plant-growth-promoting bacteria (PGPB) have shown potential in enhancing plant growth and Se accumulation. In this study, the soils collected from a Se tailing were used to isolate Se-tolerant PGPB. The results showed that a total of three strains were identified. Strain LWS1, belonging to Priestia sp., grew well in M9 medium and exhibited typical PGP characteristics by an IAA-production ability of 24.3 ± 1.37 mg·L−1, siderophore-production ability of 0.23 ± 0.04 and phosphate-solubilizing ability of 87.5 ± 0.21 mg·L−1. Moreover, LWS1 strain tolerated selenite (SeIV) up to 90 mM by a LC50 of 270.4 mg·L–1. Further investigations demonstrated that the inoculation of strain LWS1 resulted in up to 19% higher biomass and 75% higher Se concentration in rice (Oryza sativa L.) than uninoculated treatments. Our study has provided evidence that microbial Se biofortification through inoculating with Priestia sp. strain LWS1 is an alternative way to improve Se uptake in crops and maintain human health.

Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

“…The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. In recent studies, researchers have characterized different species of Priestia , including P. megaterium [ 59 ], Priestia sp., and P. endophytica [ 60 ].…”

Plant Growth Promotion and Selenium Accumulation in Zea mays by Rhizobacteria Isolated from Natural Seleniferous Soils

“…as 90 m m , which was isolated from Se contaminated soil. [ 25 ] In the present study, both L. adecarboxylata and C. neteri are capable of tolerating high concentrations of selenite (350 m m ) with IC 50 values of 225.6 and 236.6 m m , respectively.…”

“…There seems to be an association between microbial IAA production and Se tolerance as reported by Lin et al., where the bacterial‐isolate‐tolerated high Se concentrations produced a higher IAA amount. [ 25 ] One of the explanations to this behavior might be due to the associated genes often dispersing on the same plasmid. [ 36 ] Both L. adecarboxylata and C. neteri showed phosphate‐solubilizing ability with a phosphate solubilization index of 1.26 ± 0.09 and 3.29 ± 0.15, respectively.…”

“…These results are similar to other reports, demonstrating that selenite accumulates dominantly in the roots. [ 25,40 ]…”

“…Inoculation of this bacteria increased the biomass and Se content in O. sativa (rice) compared to control. [ 25 ]…”