Highways and their surrounding areas in mountainous and plateau regions are particularly susceptible to environmental changes, which can significantly impact their safety. In the context of global warming, the magnitude of environmental changes around highways has been further amplified. These environmental disturbances pose substantial risks to highway infrastructure in mountainous regions. By using satellite data and remote sensing techniques, this study focused on the environmental variations of the Du-Ku Highway (DKH) in the Tianshan Mountains and the preliminary revealed shifts in surface water, land surface temperature (LST), normalized difference vegetation index (NDVI), and temperature vegetation dryness index (TVDI) since 2000. The quantitative results showed that the water bodies with area between 0.1 and 0.5 ha showing the most significant growth around the DKH. The LST values are primarily distributed between 280 and 285 K, while the NDVI values are mostly below 0.4, and the TVDI is mainly concentrated at the two extremes. In the context of global warming and its amplified impact on mountainous and plateau regions, these findings offer critical insights that can directly support mountainous highway construction and maintenance strategies by identifying environmental indicators, providing a scientific foundation for making data-driven decisions.