Primary cell culture of meningothelial cells—a new model to study the arachnoid in glaucomatous optic neuropathy

Xin, Xiaorong; Fan, Biao; Killer, Hanspeter E.; Neutzner, Albert; Meyer, Peter

doi:10.1007/s00417-010-1372-3

Cited by 11 publications

(8 citation statements)

References 14 publications

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“…To analyse the phagocytic capacity of MECs, we evaluated the potential of these cells to engulf and take up large particles. To this end, human WHO grade I meningioma derived, hTERT immortalised Ben‐Men‐I cells were used as model for MECs as previously established (Puttmann et al, ; Xin et al, ). As shown in Figure A, MECs incubated with increasing amounts of fluorescently labelled carboxylate‐modified latex beads displayed increasing intracellular fluorescence.…”

Section: Resultsmentioning

confidence: 99%

Meningothelial cells as part of the central nervous system host defence

Li¹,

Fang²,

Killer

et al. 2013

Biology of the Cell

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Section: Resultsmentioning

confidence: 99%

Meningothelial cells as part of the central nervous system host defence

Li¹,

Fang²,

Killer

et al. 2013

Biology of the Cell

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“…The increase of CSF pressure leads to proliferation of meningothelial cells [281,282]. These cells participate in immunological processes in the CSF [283].…”

Section: Pvd and Ophthalmic Diseasesmentioning

confidence: 99%

The primary vascular dysregulation syndrome: implications for eye diseases

2013

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Vascular dysregulation refers to the regulation of blood flow that is not adapted to the needs of the respective tissue. We distinguish primary vascular dysregulation (PVD, formerly called vasospastic syndrome) and secondary vascular dysregulation (SVD). Subjects with PVD tend to have cold extremities, low blood pressure, reduced feeling of thirst, altered drug sensitivity, increased pain sensitivity, prolonged sleep onset time, altered gene expression in the lymphocytes, signs of oxidative stress, slightly increased endothelin-1 plasma level, low body mass index and often diffuse and fluctuating visual field defects. Coldness, emotional or mechanical stress and starving can provoke symptoms. Virtually all organs, particularly the eye, can be involved. In subjects with PVD, retinal vessels are stiffer and more irregular, and both neurovascular coupling and autoregulation capacity are reduced while retinal venous pressure is often increased. Subjects with PVD have increased risk for normal-tension glaucoma, optic nerve compartment syndrome, central serous choroidopathy, Susac syndrome, retinal artery and vein occlusions and anterior ischaemic neuropathy without atherosclerosis. Further characteristics are their weaker blood–brain and blood-retinal barriers and the higher prevalence of optic disc haemorrhages and activated astrocytes. Subjects with PVD tend to suffer more often from tinnitus, muscle cramps, migraine with aura and silent myocardial ischaemic and are at greater risk for altitude sickness. While the main cause of vascular dysregulation is vascular endotheliopathy, dysfunction of the autonomic nervous system is also involved. In contrast, SVD occurs in the context of other diseases such as multiple sclerosis, retrobulbar neuritis, rheumatoid arthritis, fibromyalgia and giant cell arteritis. Taking into consideration the high prevalence of PVD in the population and potentially linked pathologies, in the current article, the authors provide recommendations on how to effectively promote the field in order to create innovative diagnostic tools to predict the pathology and develop more efficient treatment approaches tailored to the person.

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“…Primary meningothelial cells derived from porcine optic nerves (PMECs) [16] were incubated with apoptotic bodies derived from monocytic U-937 or neuron-like SH-SY5Y cells and the uptake of apoptotic bodies was analyzed by confocal microscopy (Figure 1) as well as flow cytometry (Additional file 1: Figure S1). Primary MECs were found to actively ingest apoptotic bodies derived from monocytic (Figure 1A) as well as neuronal-like cells (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

“…Primary porcine meningothelial cells (PMECs) were cultured as described previously by us [16]. Monocyte-like U-937 cells (Sigma-Aldrich, 85011440) were cultured in RPMI 1640 supplemented with 10% FCS, 2 mM L-glutamine, and 1 mM sodium pyruvate (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory response following uptake of apoptotic bodies by meningothelial cells

Fang²,

Meyer³

et al. 2014

J Neuroinflammation

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BackgroundMeningothelial cells (MECs) are the cellular components of the meninges. As such, they provide important barrier function for the central nervous system (CNS) building the interface between neuronal tissue and the cerebrospinal fluid (CSF), and are also part of the immune response of the CNS.MethodsHuman, immortalized MECs were analyzed by flow cytometry and confocal microscopy to study the uptake of apoptotic cells. Furthermore, cytokine and chemokine production by MECs was analyzed by cytokine array and ELISA.ResultsWe found that MECs are highly active phagocytes able of ingesting and digesting large amounts of apoptotic cells. Furthermore, the uptake of apoptotic cells by MECs was immune suppressive via inhibiting the secretion of pro-inflammatory and chemoattractant cytokines and chemokines IL-6, IL-8, IL-16, MIF, and CXCL1, while increasing the secretion of anti-inflammatory IL-1 receptor antagonist by MECs.ConclusionMECs respond with the secretion of anti-inflammatory cytokines and chemokines following the uptake of apoptotic cells potentially connecting these cells to processes important for the shut-down of immune responses in the brain.

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Primary cell culture of meningothelial cells—a new model to study the arachnoid in glaucomatous optic neuropathy

Abstract: The technique described in this paper for the primary culture of meningothelial cells from the subarachnoid space of the optic nerve and using magnetic beads for the removal of fibroblasts is effective in obtaining a highly enriched meningothelial cell culture.

Cited by 11 publications

References 14 publications

Meningothelial cells as part of the central nervous system host defence

Meningothelial cells as part of the central nervous system host defence

The primary vascular dysregulation syndrome: implications for eye diseases

Anti-inflammatory response following uptake of apoptotic bodies by meningothelial cells

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