Primary Culture of Adult Neural Progenitors

The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells

“…All chemical reagents were obtained from Sigma-Aldrich (St.-Louis, MO, USA) and all cell culture reagents were obtained from Invitrogen (Burlington, ON, Canada) unless otherwise stated. NPCs were cultured as described in [ 52 ] with some modifications. Briefly, cells were isolated from the hippocampi of postnatal day 0 – 2 (P0–P2) mouse pups.…”