Primary culture of normal pituitary cells of carp (cyprinus carpio) for the study of gonadotropin release

Ribeiro, Luiz P.; Ahne, W.; Lichtenberg, V.

doi:10.1007/bf02617992

Cited by 18 publications

(3 citation statements)

References 12 publications

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“…Recently pituitary fragments in a perifusion system have been described in goldfish (Mackenzie et al, 1984). The use df dispersed pituitary cells of the whole pituitary has been also reported either in short-term incubation (rainbow trout: Fihraeus-van Ree et al, 1982a,b) or in primary culture in a static model (rainbow trout: Fkhraeusvan Ree et al, 1982b; carp: Ribeiro and Ahne, 1982;Ribeiro et al, 1983) and in a perifusion system (goldfish: Chang et al, 1984). Recently a primary culture of purified pituitary gonadotrophs have been set up for African catfish (Leeuw et al, 1984).…”

Section: Dlscusslonmentioning

confidence: 99%

Use of pituitary cells in primary culture to study the regulation of gonadotropin hormone (GtH) secretion in rainbow trout: Setting up and validating the system as assessed by its responsiveness to mammalian and salmon gonadotropin releasing hormone

Weil

Hansen

Hyam

et al. 1986

General and Comparative Endocrinology

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To study the regulation of gonadotropin secretion in rainbow trout in vitro, a method for preparing primary cultures of dispersed pituitary cells is described. Cells were dispersed by collagenase 0.1% in Hank's saline solution for 20 hr at 12 degrees and a high yield of viable cells was obtained. Attempts to improve cell functioning were made by varying culture conditions (density of cells initially plated, age of the culture). Cell functioning was assessed by their ability to respond to increasing doses of mammalian and salmon GnRH. Pituitaries were collected from spermiating males whose pituitaries are known to be sensitive to mammalian GnRH in vivo. Using 96-well plates, optimal conditions for good biological activity, are initial plating with 6.2 X 10(4) cells, incubation with GnRH for 24 hr on the third day after plating. In these conditions mammalian analog and salmon GnRH induced an increase in GtH release for doses ranging from 10(-9) to 10(-6) M. The GtH released during the GnRH incubation period does not decrease the sensitivity of the system since addition of 20 ng of GtH at the beginning of incubation does not modify the response profile.

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Section: Dlscusslonmentioning

confidence: 99%

Use of pituitary cells in primary culture to study the regulation of gonadotropin hormone (GtH) secretion in rainbow trout: Setting up and validating the system as assessed by its responsiveness to mammalian and salmon gonadotropin releasing hormone

Weil

Hansen

Hyam

et al. 1986

General and Comparative Endocrinology

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“…However, the authors do not specify the exact number of cells found and they worked on immature fish, in which it has now been well established that testosterone has a positive action on pituitary (for review, see . The only work performed on adult fish, using cultured pituitary cells treated with steroids, was conducted on pooled male and female carps and the authors did not specify the effect of the steroid treatments on cell number (Ribeiro et al, 1983).…”

Section: Gnrh-inducedmentioning

confidence: 99%

Cultured pituitary cell GtH response to GnRH at different stages of rainbow trout oogenesis and influence of steroid hormones

Weil¹,

Marcuzzi²

1990

General and Comparative Endocrinology

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In rainbow trout, a variable in vivo pituitary sensitivity to GnRH has been previously observed, depending on the stage of oogenesis. The purpose of the present work was to study, in vitro, the role of oestradiol (E2) and 17 alpha-hydroxy,20 beta-dihydroprogesterone (17 alpha 20 beta P), respectively, involved in vitellogenesis and in oocyte maturation, upon this variability. The study was performed using primary cultures of whole pituitary cells from animals at different stages of oogenesis and subjected to increasing doses of salmon GnRH (sGnRH) after a 3-day pretreatment with control medium or medium supplemented with the steroid at levels corresponding to those circulating at the time of particular events of the sexual cycle (maturation and vitellogenesis). In control cultures, pituitary GtH responsiveness to sGnRH was maximal at ovulation, since at this time the gonadotrophs were able to respond to 10(-9) M sGnRH, whereas during vitellogenesis and preovulatory stages, the minimal effective dose of sGnRH ranged between 10(-6) and -8 M. We have demonstrated that 17 alpha 20 beta P has a positive or negative effect by acting directly on pituitary cell responsiveness to sGnRH, depending on the stages at which it is applied; its effect is positive during early vitellogenic and preovulatory stages whereas it is negative at the time of ovulation. E2 also increased pituitary responsiveness to sGnRH when applied during early vitellogenesis at low doses, corresponding to circulating levels at the time of ovulation; higher levels of E2, corresponding to circulating levels found during the last stages of vitellogenesis, did not modify pituitary responsiveness but increased cell GtH content.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…In vitro studies have also shown that GnRH is able to release GtH from perifused pituitary fragments and dispersed pituitary cells of goldfish , and from cultured dispersed pituitary cells of carp (Ribeiro et al, 1983) and rainbow trout (Weil et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Calcium ions as a mediator in GnRH action on gonadotropin release in the common carp (Cyprinus carpio L)

Mikołajczyk¹,

Weil²,

Epler³

et al. 1990

Reprod. Nutr. Dévelop.

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Primary culture of normal pituitary cells of carp (cyprinus carpio) for the study of gonadotropin release

Cited by 18 publications

References 12 publications

Use of pituitary cells in primary culture to study the regulation of gonadotropin hormone (GtH) secretion in rainbow trout: Setting up and validating the system as assessed by its responsiveness to mammalian and salmon gonadotropin releasing hormone

Use of pituitary cells in primary culture to study the regulation of gonadotropin hormone (GtH) secretion in rainbow trout: Setting up and validating the system as assessed by its responsiveness to mammalian and salmon gonadotropin releasing hormone

Cultured pituitary cell GtH response to GnRH at different stages of rainbow trout oogenesis and influence of steroid hormones

Calcium ions as a mediator in GnRH action on gonadotropin release in the common carp (Cyprinus carpio L)

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