Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia

Zhou, Bingsheng; Liu, Chunsheng; Wang, Jingxian; Wu, Renren

doi:10.1016/j.aquatox.2006.07.021

Cited by 46 publications

(30 citation statements)

References 43 publications

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“…The isolation of fresh hepatocytes was carried out in accordance with the method developed by Kim & Takemura (2003) and modified by Zhou et al (2006). Briefly, the fresh liver was carefully excised and transferred onto a glass petri dish, and rinsed twice with phosphate buffered saline (PBS: 136.9 mM NaCl; 5.4 mM KCl; 0.81 mM MgSO 4 ; 0.44 mM KH 2 PO 4 ; 0.33 mM Na 2 HPO 4 ; 5.0 mM NaHCO 3 , pH 7.6) without Ca 2+ .…”

Section: Methodsmentioning

confidence: 99%

Effect of RRR-α-tocopherol succinate on the meat quality and antioxidative status in broilers

Zhang¹,

Wang²,

Zhou³

et al. 2012

SA J. An. Sci.

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________________________________________________________________________________ AbstractThe objective of this study was to compare the effects of two esters of α-tocopherol, all-rac-α-tocopherol acetate (DL-α-TOA) and RRR-α-tocopherol succinate (D-α-TOS), on meat quality and the antioxidative status in chicks. A total of 320 day-old Arbor Acres broiler chicks were randomly allocated to 4 treatments, each consisting of 8 pens of 10 chicks per pen. Birds in the control group received the basal diet supplemented with 30 mg DL-α-TOA/kg diet. In the other treatments the diet was supplemented with D-α-TOS at 15 mg/kg, 30 mg/kg or 60 mg/kg (TOS1, TOS2 and TOS3 treatments), respectively. The trial lasted 42 days. Positive correlations existed between dietary D-α-TOS levels and plasma and hepatic α-tocopherol concentrations, and a negative correlation with malonaldehyde (MDA) concentrations. In comparison with the control group, 30 mg/kg and 60 mg/kg of dietary D-α-TOS supplementation resulted in an increase in glutathione peroxidase (GSH-Px) activity and glutathione (GSH) content of the breast and thigh muscle and total superoxide dismutase (T-SOD) activity in the thigh muscle. Furthermore, the muscle MDA and hepatic reactive oxygen species (ROS) levels were reduced. As for meat quality, 48 h drip loss and shear force of breast and leg muscle were lower in broilers in the TOS1, TOS2 treatments and also the cooking loss in leg muscle. The study suggests that 30 mg/kg to 60 mg D-α-TOS/kg of the diet could enhance the antioxidant capacity of broiler meat, and its water-holding capacity and tenderness, in association with the reduction in lipid peroxidation as measured as a decrease in MDA and ROS concentrations.________________________________________________________________________________

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Section: Methodsmentioning

confidence: 99%

Effect of RRR-α-tocopherol succinate on the meat quality and antioxidative status in broilers

Zhang¹,

Wang²,

Zhou³

et al. 2012

SA J. An. Sci.

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“…Seldom have we observed a significant reduction in TER for DSI exposed to metals resuspended in water in the laboratory (Walker et al, 2008) or to natural waters contaminated with metals (M. Minghetti, S.S., M. A. Chadwick, C.H. and N.R.B., unpublished results) for 24 h. Likewise, Zhou et al (Zhou et al, 2006) observed no change in TER on exposure to aryl hydrocarbon receptor (AhR) agonist toxicants; thus, other end points maybe more appropriate as a measure of a toxic response. …”

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confidence: 79%

“…This can provide a better understanding of the toxicokinetics of compounds as they cross the gill epithelium. Gill cells contain the phase one enzyme cytochrome P4501A, whose activity can be measured as 7-ethoxyresorufin-Odeethylase (EROD), and Zhou et al (Zhou et al, 2006) compared the response of Nile tilapia DSI and primary hepatocyte cultures to 1,3,7,8-tetrachlorodibenzo-p-diozin (TCDD), benzo[a]pyrene (BaP), polychlorinated biphenyl (PCB) mixture (Aroclor 1254) and polybrominated diphenyl ether (PBDE) mixture (DE71). Both hepatocytes and DSI exhibited a good dose-response curve to TCDD, BaP and PCBs, but not to PBDEs, generating 24 h EC 50 values in the 10 −6 to 10 −9 mol l −1 range.…”

Section: Toxicity Testsmentioning

confidence: 99%

“…Abcb1 (Pgp) is capable of exporting non-metabolised xenobiotics, whereas abcb11, abcc1-5, also known as the multidrug resistant associated protein (MRP), and abcg2 export products of phase II metabolism (Xu et al, 2005). In the primary gill cells the cultured branchial epithelium possesses the CYP450A1 enzymes necessary for xenobiotic biotransformation (Carlsson et al, 1999;Zhou et al, 2006;Leguen et al, 2000; Jӧnsson et al, 2006) and in the RT-gill-W1 cell line there is a high expression of abcc1, abcc2, abcc3 and abcc5 (Fischer et al, 2011). However, in another study, only abcc3 was detected in gill tissues (Lončar et al, 2010).…”

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confidence: 99%

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Gill cell culture systems as models for aquatic environmental monitoring

Bury

Schnell

Högstrand

2014

Journal of Experimental Biology

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A vast number of chemicals require environmental safety assessments for market authorisation. To ensure acceptable water quality, effluents and natural waters are monitored for their potential harmful effects. Tests for market authorisation and environmental monitoring usually involve the use of large numbers of organisms and, for ethical, cost and logistic reasons, there is a drive to develop alternative methods that can predict toxicity to fish without the need to expose any animals. There is therefore a great interest in the potential to use cultured fish cells in chemical toxicity testing. This review summarises the advances made in the area and focuses in particular on a system of cultured fish gill cells grown into an epithelium that permits direct treatment with water samples. KEY WORDS: FIGCS, Biomonitoring, Environmental risk assessment, Fish, In vitro, Toxicology IntroductionThe Industrial Revolution caused a rapid rise in the use of raw materials and urbanisation as the populace moved to the cities for employment. Since this time, there has been a continuous increase in living standards that to a large part has been fuelled by innovations within the chemical and pharmaceutical industry. Life expectancy has increased as a result of great advances in medical practices and effective drugs against many fatal diseases. The increase in life expectancy has seen the population of the world grow, reaching 7 billion in 2012, and to feed this population there have been great advances in agricultural productivity partly via the development of pesticides and nitrate/phosphate-based fertilisers. These activities have altered the geochemical cycling of elements, increasing or decreasing concentrations in earth system compartments and increasing global distribution (Doney, 2010). Anthropogenic activities have left a cumulative and lasting impression on the biosphere -so much so that geologists have termed the current epoch the anthropocene (Zalasiewicz et al., 2010).The increase in agricultural and industrial production and consumption of raw materials has resulted in the creation of vast amounts of waste that enters the aquatic ecosystem. An acknowledgement of the decline in environmental quality due to contaminants has led to the development of environmental quality standards (EQS) in many countries, and to assess whether these standards are being adhered to, many jurisdictions also have a programme of waste water effluent testing (WET) and/or biomonitoring. The EQS are derived from toxicity tests that use numerous organisms per compound, and in order to set standards several species are tested. In the USA ~3 million fish are used in WET procedures (see Tanneberger et al., 2013). There is a move towards reducing the number of animals used in research and toxicology studies and there are a number of international initiatives aimed at investigating the 3Rs -reduction, replacement and refinement -in animal research (for example, see http://www.nc3rs.org.uk/). Within the context of the need to determine EQS fo...

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“…Also, it is possible to include mechanistic studies, and to test for toxicity that is specific to humans: sensitivity differences between humans and rodents can affect animals (Kari et al, 2007). Among the in vitro screening systems, primary cell cultures and/or target organ-specific cell lines can be used to measure the general toxicity of a test compound (Zhou et al, 2006). However, the sensitivity of hepatotoxicity using primary human hepatocytes or the HepG2 cell line cannot predict effects in early development and toxicological differences, which depend on the state of differentiation in hepatocytes (Knasmuller et al, 2004;Xu et al, 2004).…”

Section: Limitation Of Traditional Toxicity Screeningmentioning

confidence: 99%

Application of Embryonic Stem Cells as a Novel Tool in Drug Screening

Kim¹

2011

Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applicatio

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Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia

Cited by 46 publications

References 43 publications

Effect of RRR-α-tocopherol succinate on the meat quality and antioxidative status in broilers

Effect of RRR-α-tocopherol succinate on the meat quality and antioxidative status in broilers

Gill cell culture systems as models for aquatic environmental monitoring

Application of Embryonic Stem Cells as a Novel Tool in Drug Screening

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