Primary extranodal diffuse large B-cell lymphomas: Many sites, many entities? Clinico-pathological, immunohistochemical and cytogenetic study of 106 cases

Magnoli, Francesca; Bernasconi, Barbara; Vivian, Lisa F.; Proserpio, Ilaria; Pinotti, Graziella; Campiotti, Leonardo; Mazzucchelli, Luca; Sessa, Fausto; Tibiletti, Maria Grazia; Uccella, Silvia

doi:10.1016/j.cancergen.2018.08.001

Cited by 26 publications

(18 citation statements)

References 36 publications

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“…107,108,112 BCL6, BCL2 and MYC rearrangements are present in approximately 50%, 10% and 6-15%, respectively. 108,111 Immune evasion is involved in pathogenesis as shown by gains of PD-L1 and/or PD-L2 and common loss of the major histocompatibility complex. Most cases of PT-DLBCL are of activated B-cell-like type by gene expression profiling.…”

Section: Cytogenetics and Molecularmentioning

confidence: 99%

Diffuse large B-cell lymphoma variants: an update

et al. 2020

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Section: Cytogenetics and Molecularmentioning

confidence: 99%

Diffuse large B-cell lymphoma variants: an update

et al. 2020

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“…For example, high expression of the B-cell lymphoma (BCL)-6 and BCL-2 genes indicates that the prognosis of DLBCL is poor. [ 23 ] The immunohistochemistry results of patient 2 indicated positivity for BCL-2 and BCL-6, which implied that the prognosis was poor.…”

Section: Discussionmentioning

confidence: 99%

Renal metastases from esophageal cancer and retroperitoneal lymphoma detected via chromosome duplications identified by fluorescence in situ hybridization in urine exfoliated cells

Hu¹,

Ke²,

Yuanqing³

et al. 2021

Medicine

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Rationale: Renal-occupying lesions positive for urine fluorescence in situ hybridization (FISH) are usually considered urothelial carcinomas. Here, we describe 2 cases of renal metastases with chromosome duplications in urine exfoliated cells. Patient symptoms: Patient 1, a 56-year-old male with a history of esophageal cancer, was admitted to our hospital on May 2017 after presenting with right back pain with microscopic hematuria for 1 month. Magnetic resonance imaging (MRI) showed right renal space-occupying lesions (5.4 cm × 4.6 cm) and multiple enlarged lymph nodes in the right renal hilum and retroperitoneum. The cystoscopy results were negative, and FISH analysis of urine exfoliated cells was positive, indicative of chromosome 3, 7, and 17 amplifications. Patient 2 was a 50-year-old male who was admitted to our hospital on May 2019 with no obvious cause of abdominal pain and abdominal distension (lasting for 7 days), with a serum creatinine level of 844 μmol/L. Patient 2 had no hematuria or fever, and MRI showed left renal inferior and medial space-occupying lesions, and multiple mesenteric nodules at the junction of the left adrenal gland, retroperitoneum, abdomen, and pelvis, which were partially fused. The tumor lesions were approximately 3.1 cm × 2.3 cm in size. The urine FISH results were positive, indicating chromosome 3, 7, and 17 amplifications. Diagnoses: Both patients were diagnosed with renal tumors with unknown pathology. Interventions: Patient 1 underwent laparoscopic resection of the kidney and ureter, and sleeve cystectomy. The postoperative pathological diagnosis was metastatic keratinized squamous cell carcinoma, with squamous cell carcinoma in the right hilar lymph node. Histological FISH of the primary esophageal cancer and renal metastases were consistent with the urine FISH test results. Patient 2 underwent a biopsy of the left renal inferior and retroperitoneal areas, and was diagnosed with diffuse large B-cell lymphoma. Outcomes: Patient 1 survived 6 months after urological surgery. After treating patient 2 with the R-CHOP regimen and kinase inhibitors, his renal function recovered significantly and the mass become undetectable. Lessons: Our results imply that FISH-positive renal occupying lesions should be considered as potential renal metastases with chromosome aberrations when making a differential diagnosis.

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“…This simplified analysis and the optimized FISH protocols might be reasons for the lower cut-off values for our probes as compared to those described in the literature (Table 1 A) [ 5 ]. However, the methods used for the assessment of the thresholds were not indicated in all reports [ 6 – 9 ].…”

Section: Discussionmentioning

confidence: 99%

Optimized workflow for digitalized FISH analysis in pathology

Chea¹,

Pleiner²,

Schweizer³

et al. 2021

Diagn Pathol

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Background Effective workflow management in a diagnostic pathology laboratory is critical to achieve rapid turnover while maintaining high quality. Fluorescence in situ hybridization analysis (FISH) is the preferred technique for detecting single chromosomal aberrations in diagnostic surgical pathology. Material and methods FISH analysis applying a rapid hybridization protocol and using an automated whole-slide fluorescence scanning device (3DHISTECH, Sysmex, Switzerland) were implemented in our workflow. By analyzing 42 diagnostic cases, effects of two different scanning profiles on scanning time, and device memory usage were investigated. Manual signal counting (CaseViewer) and software based signal counting (FISHQuant) were compared. Results The two scanning profiles, both including a Z-stack function, differed in their exposure time and digital gain. The “low profile” setting (LP) resulted in a significantly shorter scanning time and lower storage volume compared to the “high profile” (HP) setting, making the LP ideal for routine applications. Both signal counting methods (manual versus software based) provided similar cut-offs on a test-cohort of 13 samples. Conclusion Scanning FISH slides provides good picture quality, reduces the analysis time and allows easy picture archiving and facilitates remote diagnostics, allowing an effective workflow.

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Primary extranodal diffuse large B-cell lymphomas: Many sites, many entities? Clinico-pathological, immunohistochemical and cytogenetic study of 106 cases

Cited by 26 publications

References 36 publications

Diffuse large B-cell lymphoma variants: an update

Diffuse large B-cell lymphoma variants: an update

Renal metastases from esophageal cancer and retroperitoneal lymphoma detected via chromosome duplications identified by fluorescence in situ hybridization in urine exfoliated cells

Optimized workflow for digitalized FISH analysis in pathology

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