2008
DOI: 10.1152/ajpcell.00239.2008
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Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling

Abstract: The actin cytoskeleton regulates exocytosis in all secretory cells. In neutrophils, Rac2 GTPase has been shown to control primary (azurophilic) granule exocytosis. In this report, we propose that Rac2 is required for actin cytoskeletal remodeling to promote primary granule exocytosis. Treatment of neutrophils with low doses (< or = 10 microM) of the actin-depolymerizing drugs latrunculin B (Lat B) or cytochalasin B (CB) enhanced both formyl peptide receptor- and Ca(2+) ionophore-stimulated exocytosis. Higher c… Show more

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Cited by 94 publications
(97 citation statements)
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“…Because both subsets showed significant changes in nutrient transporter expression, we tested whether in vitro neutrophil activation and induction of granule exocytosis could alter surface expression of nutrient transporters. As previously reported (33), efficient secondary and primary granule exocytosis was triggered by the combined treatment with LB and fMLF and evidenced by marked increases in the CD66b and CD63 surface markers (5.66-6.87-fold and 23.74-29.4-fold, respectively, Supplemental Table II). In contrast, upon LB+fMLF treatment, nutrient transporter levels in HC and CF neutrophils remained within a range (0.61-1.67-fold) of those observed in control conditions.…”
Section: Increased Surface Expression Of Nutrient Transporters Is Notsupporting
confidence: 81%
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“…Because both subsets showed significant changes in nutrient transporter expression, we tested whether in vitro neutrophil activation and induction of granule exocytosis could alter surface expression of nutrient transporters. As previously reported (33), efficient secondary and primary granule exocytosis was triggered by the combined treatment with LB and fMLF and evidenced by marked increases in the CD66b and CD63 surface markers (5.66-6.87-fold and 23.74-29.4-fold, respectively, Supplemental Table II). In contrast, upon LB+fMLF treatment, nutrient transporter levels in HC and CF neutrophils remained within a range (0.61-1.67-fold) of those observed in control conditions.…”
Section: Increased Surface Expression Of Nutrient Transporters Is Notsupporting
confidence: 81%
“…Blood was washed and resuspended in medium (serum-free RPMI 1640) at 5 3 10 6 /ml and incubated with LB (1.25 mM) for 5 min at 37˚C, followed by fMLF (5 mM) for 10 min at 37˚C. In these conditions, primary and secondary neutrophil granules (and tertiary granules) are exocytosed to a large extent (33). Incubations were stopped by washing the cells with ice-cold PBS-EDTA and transferring tubes on ice.…”
Section: Exocytosis and Nutrient Transporter Surface Expression Assaysmentioning
confidence: 99%
“…Coactosin-like protein, which belongs to the actin-depolymerizing factor family of actin-binding proteins, interacts with 5-lipoxygenase and filamentous actin; because extensive compositional re-organization is exhibited in PMN during initial stages of phenotypic conversion (Stie & Jesaitis, 2007), upon PMN activation, the assembly of polarized F-actin promotes exocytosis (Mitchell et al, 2008). Actin (de)polymerizetion is also important for the process of transendothelial migration, the means by which leukocytes exit the blood and enter tissues -a key step in inflammatory processes in organs like the lungs.…”
Section: Discussionmentioning
confidence: 99%
“…Cytochalasin b and nocodazole have previously been shown to inhibit the uptake of particles during neutrophil mediated phagocytosis [15]. In order to evaluate the effect of cytochalasin b and nocodazole on the ability of haemocytes to phagocytose pathogens, haemocytes were extracted and exposed to cytochalasin b and/or nocodazole for 30 min prior to co-incubation with conidia of A. flavus.…”
Section: Effect Of Nocodazole and Cytochalasin B On Haemocyte Mediatementioning
confidence: 99%