Primary HCMV infection in pregnancy from classic data towards metabolomics: An exploratory analysis

Fattuoni, Claudia; Palmas, Francesco; Noto, Antonio; Barberini, Luigi; Mussap, Michele; Grapov, Dmitry; Dessì, Angelica; Casu, Mariano; Casanova, Andrea; Furione, Milena; Arossa, Alessia; Spinillo, Arsenio; Baldanti, Fausto; Fanos, Vassilios; Zavattoni, Maurizio

doi:10.1016/j.cca.2016.06.005

Cited by 26 publications

(15 citation statements)

References 39 publications

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“…These investigators demonstrated that levels of serum L -threonine, as well as a vitamin D binding protein, perfectly stratified Ebola patients by outcome, providing better predictive ability than viral load. The amniotic fluid of human cytomegalovirus infected (HCMV) women shows elevated levels of Lthreonine compared to non-HCMV infected women [6]. No significance difference in vertical transmission of HCMV based on elevated L -threonine was observed.…”

Section: Discussionmentioning

confidence: 97%

Elevated L-Threonine is a Biomarker for Lassa Fever and Ebola

Gale

Schieffelin

Branco

et al. 2020

Preprint

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Background: Lassa fever and Ebola are characterized by non-specific initial presentations that can progress to severe multisystem illnesses with high fatality rates. Methods: Liquid Chromatography Mass Spectrometry metabolomics was used to identify and confirm metabolites disrupted in the blood of Lassa fever and Ebola patients. Results: The amino acid L-threonine is elevated during Lassa fever and Ebola. Conclusions: Metabolomics may provide tools to identify pathways that are differentially affected during viral hemorrhagic fevers and guide development of diagnostics to monitor and predict outcome.

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Section: Discussionmentioning

confidence: 97%

Elevated L-Threonine is a Biomarker for Lassa Fever and Ebola

Gale

Schieffelin

Branco

et al. 2020

Preprint

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“…Previous experimental studies have shown that HCMV infection induces changes in the host metabolism by redirecting the host's physiological functions to provide the virus with energy and precursor molecules for its own replication, thus affecting normal cellular metabolic balance and establishing a specific metabolic signature for the virus [8,[39][40][41]. Hence, detection of HCMV infection by studying the urinary metabolic profile makes sense.…”

Section: Discussionmentioning

confidence: 99%

1H-NMR Urinary Metabolic Profile, A Promising Tool for the Management of Infants with Human Cytomegalovirus-Infection

Frick¹,

Barba

Fenoy-Alejandre³

et al. 2019

Metabolites

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Congenital human cytomegalovirus (HCMV) infection is the most common mother-to-child transmitted infection in the developed world. Certain aspects of its management remain a challenge. Urinary metabolic profiling is a promising tool for use in pediatric conditions. The aim of this study was to investigate the urinary metabolic profile in HCMV-infected infants and controls during acute care hospitalization. Urine samples were collected from 53 patients at five hospitals participating in the Spanish congenital HCMV registry. Thirty-one cases of HCMV infection and 22 uninfected controls were included. Proton nuclear magnetic resonance (1H-NMR) spectra were obtained using NOESYPR1D pulse sequence. The dataset underwent orthogonal projection on latent structures discriminant analysis to identify candidate variables affecting the urinary metabolome: HCMV infection, type of infection, sex, chronological age, gestational age, type of delivery, twins, and diet. Statistically significant discriminative models were obtained only for HCMV infection (p = 0.03) and chronological age (p < 0.01). No significant differences in the metabolomic profile were found between congenital and postnatal HCMV infection. When the HCMV-infected group was analyzed according to chronological age, a statistically significant model was obtained only in the neonatal group (p = 0.01), with the differentiating metabolites being betaine, glycine, alanine, and dimethylamine. Despite the considerable variation in urinary metabolic profiles in a real-life setting, clinical application of metabolomics to the study of HCMV infection seems feasible.

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“…In conclusion, metabolomics could describe maternal and fetal status in congenital HCMV infection, allowing an early diagnosis and an accurate management [34], and, in our opinion, it could be also applied in the evaluation of breast milk acquired CMV infection [35].…”

Section: Metabolomics In Obstetricsmentioning

confidence: 92%

“…As evidenced, through GC-MS analysis of AF, different profiles characterize pregnant women that transmitted HMCV infection to their fetuses (n = 20), instead of mothers who acquired but not transmitted the virus (n = 20) and healthy controls (n = 23). Moreover, AF samples from mothers whose neonates resulted symptomatic at birth (n = 9) were clearly separated from AF belonging to neonates who acquired HCMV infection but did not show clinical signs, especially regarding metabolites related to fatty acids biosynthesis [34].…”

Section: Metabolomics In Obstetricsmentioning

confidence: 98%

How could metabolomics change pediatric health?

Bardanzellu

Fanos

2020

Ital J Pediatr

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In the last years, 'omics' technologies, and especially metabolomics, emerged as expanding scientific disciplines and promising technologies in the characterization of several pathophysiological processes. In detail, metabolomics, able to detect in a dynamic way the whole set of molecules of low molecular weight in cells, tissues, organs, and biological fluids, can provide a detailed phenotypic portray, representing a metabolic "snapshot." Thanks to its numerous strength points, metabolomics could become a fundamental tool in human health, allowing the exact evaluation of individual metabolic responses to pathophysiological stimuli including drugs, environmental changes, lifestyle, a great number of diseases and other epigenetics factors. Moreover, if current metabolomics data will be confirmed on larger samples, such technology could become useful in the early diagnosis of diseases, maybe even before the clinical onset, allowing a clinical monitoring of disease progression and helping in performing the best therapeutic approach, potentially predicting the therapy response and avoiding overtreatments. Moreover, the application of metabolomics in nutrition could provide significant information on the best nutrition regimen, optimal infantile growth and even in the characterization and improvement of commercial products' composition. These are only some of the fields in which metabolomics was applied, in the perspective of a precision-based, personalized care of human health. In this review, we discuss the available literature on such topic and provide some evidence regarding clinical application of metabolomics in heart diseases, auditory disturbance, nephrouropathies, adult and pediatric cancer, obstetrics, perinatal conditions like asphyxia, neonatal nutrition, neonatal sepsis and even some neuropsychiatric disorders, including autism. Our research group has been interested in metabolomics since several years, performing a wide spectrum of experimental and clinical studies, including the first metabolomics analysis of human breast milk. In the future, it is reasonable to predict that the current knowledge could be applied in daily clinical practice, and that sensible metabolomics biomarkers could be easily detected through cheap and accurate sticks, evaluating biofluids at the patient's bed, improving diagnosis, management and prognosis of sick patients and allowing a personalized medicine. A dream? May be I am a dreamer, but I am not the only one.

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Primary HCMV infection in pregnancy from classic data towards metabolomics: An exploratory analysis

Cited by 26 publications

References 39 publications

Elevated L-Threonine is a Biomarker for Lassa Fever and Ebola

Elevated L-Threonine is a Biomarker for Lassa Fever and Ebola

1H-NMR Urinary Metabolic Profile, A Promising Tool for the Management of Infants with Human Cytomegalovirus-Infection

How could metabolomics change pediatric health?

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