Primary Human Colonic Mucosal Barrier Crosstalk with Super Oxygen-Sensitive Faecalibacterium prausnitzii in Continuous Culture

Zhang, Jianbo; Huang, Yu-Ja; Yoon, Jun Young; Kemmitt, John; Wright, C. Wayne; Schneider, Kirsten; Sphabmixay, Pierre; Hernandez-Gordillo, Victor; Holcomb, Steven J.; Bhushan, Brij M.; Rohatgi, Gar; Benton, Kyle; Carpenter, David; Kester, Jemila C.; Eng, George; Breault, David T.; Yılmaz, Ömer H.; Taketani, Mao; Voigt, Christopher A.; Trumper, David L.; Griffith, Linda G.

doi:10.1016/j.medj.2020.07.001

Cited by 85 publications

(93 citation statements)

References 118 publications

(139 reference statements)

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“…Through a radial oxygen gradient generated by the system, intestinal cells were oxygenated whereas anaerobic conditions allowed microbiota growth, as assessed by real-time monitoring via integrated noninvasive oxygen sensors [19,36]. Similar physiological hypoxia conditions were achieved by Zhang and coworkers who cocultured oxygen super-sensitive bacterial species using a differently designed MPS, the GuMi (Figure 3C) [37]. This platform induced a steep oxygen gradient through the addition of a long-term continuous flow of anoxic apical medium and aerobic basal media.…”

Section: Trends In Biotechnologymentioning

confidence: 70%

“…(C) Schematic representation of the GuMi physiome platform. Intestinal epithelial cells are cocultured in standardized Transwell® with anaerobic bacteria by using apical anoxic media and basolateral aerobic media applied to the system with individual flow control (image adapted, with permission, from [37]). (D) A Transwell® format MPS configuration allows the integration of up to 10 organ tissues (image reproduced, with permission, from [50]).…”

Section: En Route To Replicating the Gut-kidney Axis Using Mpssmentioning

confidence: 99%

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Microphysiological Systems to Recapitulate the Gut–Kidney Axis

Giordano

Mihăilă

Amirabadi

et al. 2021

Trends in Biotechnology

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Chronic kidney disease (CKD) typically appears alongside other comorbidities, highlighting an underlying complex pathophysiology that is thought to be vastly modulated by the bidirectional gut-kidney crosstalk. By combining advances in tissue engineering, biofabrication, microfluidics, and biosensors, microphysiological systems (MPSs) have emerged as promising approaches for emulating the in vitro interconnection of multiple organs, while addressing the limitations of animal models. Mimicking the (patho)physiological states of the gut-kidney axis in vitro requires an MPS that can simulate not only this direct bidirectional crosstalk but also the contributions of other physiological participants such as the liver and the immune system. We discuss recent developments in the field that could potentially lead to in vitro modeling of the gut-kidney axis in CKD. Chronic Kidney Disease: A Metabolic Disorder with Disrupted Inter-Organ and Inter-Organismal SignalingChronic kidney disease (CKD) is the most widespread kidney disease and is characterized by the gradual loss of organ function over time, which impairs the ability to filter metabolic waste products from the blood (Box 1). The kidneys have many highly specialized functions, such as blood filtration and active secretion for the removal of metabolic waste, reabsorption of essential nutrients, maintenance of blood volume and electrolyte homeostasis, and metabolic and endocrine activity [1]. HighlightsGut microbiota-derived metabolites are key molecular mediators of the microbiota-host axis.The excretory capacity of the kidney is an essential part of human gut microbial symbiosis.Chronic kidney disease (CKD) is a metabolic disease in which gut microbiotaderived metabolites accumulate in the blood and adversely affect host physiological functions.Intestine-on-a-chip models have been developed that recapitulate the 3D epithelial barrier, the gut-microbiome interaction, and intercellular crosstalk with remote organs.Components of the immune system are pivotal in remote communication between the gut and kidney.The combination of engineered microphysiological systems with highthroughput multiomic analysis will provide novel insights into organ intercommunication in CKD.

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Section: Trends In Biotechnologymentioning

confidence: 70%

Section: En Route To Replicating the Gut-kidney Axis Using Mpssmentioning

confidence: 99%

Microphysiological Systems to Recapitulate the Gut–Kidney Axis

Giordano

Mihăilă

Amirabadi

et al. 2021

Trends in Biotechnology

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“…In another study, Zhang et al developed a Gut-Microbiome physiome platform featuring primary human colon epithelial cells and oxygen-sensitive commensal anaerobe ( Faecalibacterium prausnizii ) to investigate mucosal barrier-bacterial interactions. Under flow condition, it has been demonstrated that F. parausnizzi exerted anti-inflammatory effects through the production of butyrate and subsequently downregulated toll-like receptor (TLR)-3 and TLR-4, responsible for activation of nuclear factor-light-chain-enhancer of activated B cells (NF-KB) signaling and inflammation [ 226 ]. In another organ-on-a-chip model featuring liver (human hepatocytes and Kupfer cells) and gut (ulcerative colitis epithelium, dendritic cells, and macrophages), Trapecar et al showed the involvement of effector CD4 T cells in modulating cellular metabolism and inflammatory response [ 227 ].…”

Section: Organ-on-a-chip Platforms For Assessment Of Nanoparticle Toxmentioning

confidence: 99%

Organ-on-a-chip platforms for evaluation of environmental nanoparticle toxicity

Radišić

2021

Bioactive Materials

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Despite showing a great promise in the field of nanomedicine, nanoparticles have gained a significant attention from regulatory agencies regarding their possible adverse health effects upon environmental exposure. Whether those nanoparticles are generated through intentional or unintentional means, the constant exposure to nanomaterials can inevitably lead to unintended consequences based on epidemiological data, yet the current understanding of nanotoxicity is insufficient relative to the rate of their emission in the environment and the lack of predictive platforms that mimic the human physiology. This calls for a development of more physiologically relevant models, which permit the comprehensive and systematic examination of toxic properties of nanoparticles. With the advancement in microfabrication techniques, scientists have shifted their focus on the development of an engineered system that acts as an intermediate between a well-plate system and animal models, known as organ-on-a-chips. The ability of organ-on-a-chip models to recapitulate in vivo like microenvironment and responses offers a new avenue for nanotoxicological research. In this review, we aim to provide overview of assessing potential risks of nanoparticle exposure using organ-on-a-chip systems and their potential to delineate biological mechanisms of epidemiological findings.

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“…For example, the native intestine is more complex than the simple epithelium. Incorporation of components of the normal host microenvironment such as endothelial, immune, and neuronal cells and the microbiome, which can result in enhanced or reduced replication of different infecting microbes, will validate whether these cultures can fully recapitulate human mucosal tissues where most infections begin [62,[95][96][97][98]. While it is unclear whether genetically matched tissue (mesenchyme, immune, and neuronal cells and the HIO) will be required to establish such cultures, human organoid cocultures offer the first controllable system to understand the mechanisms of immune or neuronal cells or microbial interactions and competition leading to enhanced or inhibitory outcomes.…”

Section: Summary and Current Questionsmentioning

confidence: 99%

Organoids to Dissect Gastrointestinal Virus–Host Interactions: What Have We Learned?

Crawford

Ramani

Blutt

et al. 2021

Viruses

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Historically, knowledge of human host–enteric pathogen interactions has been elucidated from studies using cancer cells, animal models, clinical data, and occasionally, controlled human infection models. Although much has been learned from these studies, an understanding of the complex interactions between human viruses and the human intestinal epithelium was initially limited by the lack of nontransformed culture systems, which recapitulate the relevant heterogenous cell types that comprise the intestinal villus epithelium. New investigations using multicellular, physiologically active, organotypic cultures produced from intestinal stem cells isolated from biopsies or surgical specimens provide an exciting new avenue for understanding human specific pathogens and revealing previously unknown host–microbe interactions that affect replication and outcomes of human infections. Here, we summarize recent biologic discoveries using human intestinal organoids and human enteric viral pathogens.

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Primary Human Colonic Mucosal Barrier Crosstalk with Super Oxygen-Sensitive Faecalibacterium prausnitzii in Continuous Culture

Cited by 85 publications

References 118 publications

Microphysiological Systems to Recapitulate the Gut–Kidney Axis

Microphysiological Systems to Recapitulate the Gut–Kidney Axis

Organ-on-a-chip platforms for evaluation of environmental nanoparticle toxicity

Organoids to Dissect Gastrointestinal Virus–Host Interactions: What Have We Learned?

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