Primary metabolism in an Amaranthus palmeri population with multiple resistance to glyphosate and pyrithiobac herbicides

Barco-Antoñanzas, María; Gil‐Monreal, Miriam; Eceiza, Mikel Vicente; Royuela, M.; Zabalza, Ana

doi:10.1016/j.plantsci.2022.111212

Cited by 9 publications

(12 citation statements)

References 65 publications

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“…The mutant allele is dominant, and is not supposed to alter ALS functionality significantly (Yu et al, 2010). Moreover, according to previous studies, Trp-574 mutation does not endow the plants with additional physiological traits, as mutated and unmutated plants show similar growth and total amino acid, soluble sugar, and starch contents (Barco-Antoñanzas et al, 2022). This means that the mutation confers an important cross-resistance to ALS inhibitors with apparently negligible fitness costs (Tranel and Wright, 2002).…”

Section: Discussionmentioning

confidence: 94%

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Role of oxidative stress in the physiology of sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting acetolactate synthase

et al. 2023

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The aim of the present study was to elucidate the role of oxidative stress in the mode of action of acetolactate synthase (ALS) inhibiting herbicides. Two populations of Amaranthus palmeri S. Watson from Spain (sensitive and resistant to nicosulfuron, due to mutated ALS) were grown hydroponically and treated with different rates of the ALS inhibitor nicosulfuron (one time and three times the field recommended rate). Seven days later, various oxidative stress markers were measured in the leaves: H2O2, MDA, ascorbate and glutathione contents, antioxidant enzyme activities and gene expression levels. Under control conditions, most of the analysed parameters were very similar between sensitive and resistant plants, meaning that resistance is not accompanied by a different basal oxidative metabolism. Nicosulfuron-treated sensitive plants died after a few weeks, while the resistant ones survived, independently of the rate. Seven days after herbicide application, the sensitive plants that had received the highest nicosulfuron rate showed an increase in H2O2 content, lipid peroxidation and antioxidant enzymatic activities, while resistant plants did not show these responses, meaning that oxidative stress is linked to ALS inhibition. A supralethal nicosulfuron rate was needed to induce a significant oxidative stress response in the sensitive population, providing evidence that the lethality elicited by ALS inhibitors is not entirely dependent on oxidative stress.

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Section: Discussionmentioning

confidence: 94%

“…The dCAPS analyses were conducted to determine the possible mutant ALS alleles, as described before (Barco-Antoñanzas et al, 2022).…”

Section: Analytical Determinationsmentioning

confidence: 99%

Role of oxidative stress in the physiology of sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting acetolactate synthase

et al. 2023

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“…Glyphosate (commercial formula, FORTIN Green, Key, Lleida, Spain) was applied at the recommended field rate (0.84 kg ha −1 ) (Culpepper et al, 2006). The ALS inhibitor pyrithiobac, which belongs to the pyrimidinyl‐benzoate chemical family (analytical grade, Dr Ehrenstorfer LGC, Augsburg, Germany), was also applied at recommended field rate (89 g ha −1 ) (Wise et al, 2009) with 5.4% sodium lauryl sulphate adjuvant (commercial formula Biopower 27.65% [p/v]) (Bayer Crop Science, Madrid, Spain) (Barco‐Antoñanzas et al, 2022). Plants of the combined treatment were sprayed first with glyphosate and then with pyrithiobac.…”

Section: Methodsmentioning

confidence: 99%

“…The mechanism of the GR population was EPSPS gene amplification, as GR individuals had a mean 47.5‐fold increase in the number of copies of the EPSPS gene (Chandi et al, 2012; Fernández‐Escalada et al, 2016). Multiple resistant plants were glyphosate‐resistant due to EPSPS gene amplification (48‐fold increase), while resistance to ALS inhibitors was related to point mutations at five positions in the ALS gene sequence (Barco‐Antoñanzas et al, 2022). No other phenotypic differences have been detected between populations (Barco‐Antoñanzas et al, 2022; Fernández‐Escalada et al, 2016, 2017, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…The resistance mechanism of the resistant populations is usually well characterised, but the possible physiological implications and the physiological effects triggered by the exposure to glyphosate or an ALS‐inhibiting herbicide in these resistant weeds are not known. The availability of a glyphosate‐resistant population overexpressing EPSPS (Fernández‐Escalada et al, 2016) and of another population with multiple resistance to glyphosate and ALS inhibitors (due to EPSPS gene amplification and ALS point mutations) (Barco‐Antoñanzas et al, 2022), offers the opportunity to study the physiological responses of target‐site resistant biotypes to glyphosate and ALS inhibitors, and specifically the role of proteolytic activities in the mode of action and the resistance to these herbicides.…”

Section: Introductionmentioning

confidence: 99%

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Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis

Barco‐Antoñanzas,

Font‐Farre,

Eceiza

et al. 2023

Physiologia Plantarum

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The herbicides glyphosate and pyrithiobac inhibit the enzyme 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) in the aromatic amino acid biosynthetic pathway and acetolactate synthase (ALS) in the branched‐chain amino acid biosynthetic pathway, respectively. Here we characterise the protease activity profiles of a sensitive (S), a glyphosate‐resistant (GR) and a multiple‐resistant (MR) population of Amaranthus palmeri in response to glyphosate and pyrithiobac. Amino acid accumulation and cysteine protease activities were induced with both herbicides in the S population and with pyrithiobac in the GR population, suggesting that the increase in cysteine proteases is responsible for the increased degradation of the available proteins and the observed increase in free amino acids. Herbicides did not induce any changes in the proteolytic activities in the populations with target‐site resistance, indicating that this effect was only induced in sensitive plants.

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Estimating the role of maize Y-EPSPS gene in glyphosate resistance in Arabidopsis transgenic lines

Wang,

Zhang,

Wang

et al. 2024

Plant Growth Regul

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Primary metabolism in an Amaranthus palmeri population with multiple resistance to glyphosate and pyrithiobac herbicides

Cited by 9 publications

References 65 publications

Role of oxidative stress in the physiology of sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting acetolactate synthase

Role of oxidative stress in the physiology of sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting acetolactate synthase

Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis

Estimating the role of maize Y-EPSPS gene in glyphosate resistance in Arabidopsis transgenic lines

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