Primary Metabolism Is Distinctly Modulated by Plant Resistance Inducers in Coffea arabica Leaves Infected by Hemileia vastatrix

Pôssa, Kátia Ferreira; Silva, Joyce Alves Goulart; Resende, Mário Lúcio Vilela de; Tenente, Rita; Pinheiro, Carla; Chaves, Inês; Planchon, Sébastien; Monteiro, Ana Cristina Andrade; Renaut, Jenny; Carvalho, Milene Alves Figueiredo; Ricardo, Cândido Pinto; Guerra‐Guimarães, Leonor

doi:10.3389/fpls.2020.00309

Cited by 11 publications

(11 citation statements)

References 77 publications

(104 reference statements)

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“…In coffee plants, protection against coffee leaf rust was achieved by treatments with different PRIs such as yeast extract [193], Bacillus thuringiensis [194,195], exopolysaccharides from bacterial cells of Xanthomonas campestris [196], acibenzolar-S-methyl (ASM-salicylic acid functional analog belonging to the benzothiadiazole (BTH) family) [153,197], potassium silicate [198], phosphites [199] and formulations based on natural products [200][201][202]. In field trials some of these PRIs used alone, combined, or in association with fungicides can significantly reduce CLR incidence [199][200][201].…”

Section: Systemic-acquired Resistance (Sar) In Coffeementioning

confidence: 99%

“…Recently, a physiological (leaf gas-exchange), biochemical (enzymatic) and proteomic study was undertaken. Coffee leaves were treated with Greenforce CuCa (formulation prepared with coffee industry by-products supplemented with calcium and copper salts), ASM and inoculated with H. vastatrix [202]. Both PRIs prepared the plant to resist CLR, but they induced different mechanisms upon pathogen infection.…”

Section: Systemic-acquired Resistance (Sar) In Coffeementioning

confidence: 99%

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An Overview of the Mechanisms Involved in Coffee-Hemileia vastatrix Interactions: Plant and Pathogen Perspectives

et al. 2022

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Coffee is one of the most consumed beverages in the world. It is also one of the most globally traded commodities. Coffee leaf rust (CLR), caused by the biotrophic fungus Hemileia vastatrix, is the most important disease affecting Arabica coffee growing worldwide, leading to significant yield losses if no control measures are applied. A deep understanding of the complex mechanisms involved in coffee-H. vastatrix interactions, such as the pathogen variability and the mechanisms governing plant resistance and susceptibility, is required to breed efficiently for durable resistance and design new approaches for crop protection. Here we summarize our current understanding across multiple areas related to pathogen infection, variability and candidate effectors, breeding for disease resistance, and the various components of the coffee immune system, by reviewing a comprehensive body of research on CLR and the advances recently made. We also update information about the defense responses activated by the application of plant resistance inducers, a promising alternative to fungicides in the control of CLR. Moreover, we identify and discuss future directions for further research.

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Section: Systemic-acquired Resistance (Sar) In Coffeementioning

confidence: 99%

Section: Systemic-acquired Resistance (Sar) In Coffeementioning

confidence: 99%

An Overview of the Mechanisms Involved in Coffee-Hemileia vastatrix Interactions: Plant and Pathogen Perspectives

et al. 2022

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“…It was reported that in susceptible coffee plants, the H. vastatrix genes involved in sugar transport and metabolism were upregulated 15 . On the other hand, coffee plants treated with resistance inducers and challenged by H. vastatrix showed a reduced incidence of CLR disease which was related to primary metabolic adjustments, namely the up-regulation of proteins from the photosynthesis-related pathways and redox-related enzyme activities 16 . Coffee resistance to H. vastatrix has been associated with restricted fungal growth in the early stages of the infection process due to hypersensitive cell death (HR), accumulation of reactive oxygen species (ROS), haustoria encasement, and cell wall lignification 12 and references therein.…”

Section: Introductionmentioning

confidence: 99%

“… Do nuclear-encoded chloroplast proteins reflect S H phenotypes? For this we performed an in-silico analysis of selected nuclear-encoded protein families acting on chloroplasts, focusing on gene families previously highlighted as being involved in H. vastatrix resistance 16 , 20 , 21 . …”

Section: Introductionmentioning

confidence: 99%

The chloroplast protein HCF164 is predicted to be associated with Coffea SH9 resistance factor against Hemileia vastatrix

Guerra-Guimarães,

Pinheiro,

Oliveira

et al. 2023

Sci Rep

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To explore the connection between chloroplast and coffee resistance factors, designated as SH1 to SH9, whole genomic DNA of 42 coffee genotypes was sequenced, and entire chloroplast genomes were de novo assembled. The chloroplast phylogenetic haplotype network clustered individuals per species instead of SH factors. However, for the first time, it allowed the molecular validation of Coffea arabica as the maternal parent of the spontaneous hybrid “Híbrido de Timor”. Individual reads were also aligned on the C. arabica reference genome to relate SH factors with chloroplast metabolism, and an in-silico analysis of selected nuclear-encoded chloroplast proteins (132 proteins) was performed. The nuclear-encoded thioredoxin-like membrane protein HCF164 enabled the discrimination of individuals with and without the SH9 factor, due to specific DNA variants linked to chromosome 7c (from C. canephora-derived sub-genome). The absence of both the thioredoxin domain and redox-active disulphide center in the HCF164 protein, observed in SH9 individuals, raises the possibility of potential implications on redox regulation. For the first time, the identification of specific DNA variants of chloroplast proteins allows discriminating individuals according to the SH profile. This study introduces an unexplored strategy for identifying protein/genes associated with SH factors and candidate targets of H. vastatrix effectors, thereby creating new perspectives for coffee breeding programs.

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“…In addition to the gene expression tests, we assessed enzymatic activities of PAL, CAD, and POX, which are commonly determined in experiments on C. arabica responses to inoculation with Cercospora coffeicola and H. vastatrix [54,55]. For biochemical analyses, sampled leaf tissue was macerated, and 1% polyvinylpyrrolidone was added.…”

Section: Determination Of Enzyme Activitymentioning

confidence: 99%

Constitutive Defense Strategy of Coffee Under Field Conditions: A Comparative Assessment of Resistant and Susceptible Cultivars to Rust

Reichel

Resende

Monteiro³

et al. 2021

Mol Biotechnol

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Coffea arabica is the most economically important coffee species worldwide. However, its production is severely limited by diseases such as rust. The mechanisms underlying constitutive defense responses in coffee are still poorly understood, compared with induced defense mechanisms. We aimed to characterize constitutive defense responses of thirteen cultivars of C. arabica. Cultivars were classified under field conditions according to the level of resistance to rust: resistant (R), moderately resistant (MR), and susceptible (S). Based on this classification, the stability of eight reference genes (RGs) was evaluated. The most stable RGs were EF1α, APT1, and 24S. We also evaluated the expression of CaWRKY1, CaPAL1, CaCAD1, and CaPOX1, and activities of PAL, CAD, and POX, which are involved in lignin biosynthesis, and leaf content of total phenolic compounds and lignin. Gene expression and enzymatic activity were not correlated with defense metabolites in the R cultivar group but showed a negative correlation with phenolic compounds in MR cultivars. Cultivar S showed positive correlations of gene expression and enzyme activity with phenolic compounds. These results may assist coffee breeding programs regarding selection of genotypes and in optimization of rust resistance.

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Primary Metabolism Is Distinctly Modulated by Plant Resistance Inducers in Coffea arabica Leaves Infected by Hemileia vastatrix

Cited by 11 publications

References 77 publications

An Overview of the Mechanisms Involved in Coffee-Hemileia vastatrix Interactions: Plant and Pathogen Perspectives

An Overview of the Mechanisms Involved in Coffee-Hemileia vastatrix Interactions: Plant and Pathogen Perspectives

The chloroplast protein HCF164 is predicted to be associated with Coffea SH9 resistance factor against Hemileia vastatrix

Constitutive Defense Strategy of Coffee Under Field Conditions: A Comparative Assessment of Resistant and Susceptible Cultivars to Rust

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